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A comparison of native and non-urate Total Antioxidant Capacity of fasting plasma and saliva among middle-aged and older subjects.
Redox Rep. 2018 Dec; 23(1):57-62.RR

Abstract

OBJECTIVES

As plasma and salivary total antioxidant capacity (TAC) is mainly contributed by uric acid (UA), the present study measures non-urate TAC (Nu-TAC). The aim of the study was to correlate plasma native TAC, Nu-TAC and UA with their salivary analogues, and compare the UA contribution in both body fluids using two different methods.

METHODS

The study involved 55 middle-aged and older subjects (66.7 ± 4.5 years). TAC was determined simultaneously with two methods (ferric reducing ability of plasma - FRAP, 2.2-diphenyl-1-picryl-hydrazyl - DPPH and countertypes for saliva - FRAS and DPPHS test), with and without UA (native TAC and Nu-TAC, respectively). Plasma UA and salivary UA (SUA) were assessed.

RESULTS

Subjects with increased FRAP, DPPH and UA had higher FRAS, DPPHS and SUA, respectively (P < 0.05). Plasma Nu-TAC indices did not correlate with salivary Nu-TAC. The contribution of UA to the plasma and salivary DPPH tests was similar: 75.7 ± 10.3% and 75.2 ± 14.0%, respectively. However, the contribution of UA to FRAS was higher than that for FRAP (71.6 ± 13.9% vs. 64.0 ± 8.1%; P < 0.001).

DISCUSSION

Our findings suggest that saliva is a good predictor for native plasma TAC but not for Nu-TAC. UA level is comparably dominant in saliva and in plasma according to DPPH, but lower in plasma according to FRAP.

Authors+Show Affiliations

a Department of Hygiene and Health Promotion , Medical University of Lodz , Lodz , Poland.b Department of General Physiology , Medical University of Lodz , Lodz , Poland.c Faculty of Medical Laboratory , Medical University of Lodz , Lodz , Poland.d Department of Geriatrics , Medical University of Lodz , Lodz , Poland.d Department of Geriatrics , Medical University of Lodz , Lodz , Poland.e Department of Clinical Physiology , Medical University of Lodz , Lodz , Poland.a Department of Hygiene and Health Promotion , Medical University of Lodz , Lodz , Poland.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

29088986

Citation

Gawron-Skarbek, Anna, et al. "A Comparison of Native and Non-urate Total Antioxidant Capacity of Fasting Plasma and Saliva Among Middle-aged and Older Subjects." Redox Report : Communications in Free Radical Research, vol. 23, no. 1, 2018, pp. 57-62.
Gawron-Skarbek A, Prymont-Przymińska A, Sobczak A, et al. A comparison of native and non-urate Total Antioxidant Capacity of fasting plasma and saliva among middle-aged and older subjects. Redox Rep. 2018;23(1):57-62.
Gawron-Skarbek, A., Prymont-Przymińska, A., Sobczak, A., Guligowska, A., Kostka, T., Nowak, D., & Szatko, F. (2018). A comparison of native and non-urate Total Antioxidant Capacity of fasting plasma and saliva among middle-aged and older subjects. Redox Report : Communications in Free Radical Research, 23(1), 57-62. https://doi.org/10.1080/13510002.2017.1392714
Gawron-Skarbek A, et al. A Comparison of Native and Non-urate Total Antioxidant Capacity of Fasting Plasma and Saliva Among Middle-aged and Older Subjects. Redox Rep. 2018;23(1):57-62. PubMed PMID: 29088986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparison of native and non-urate Total Antioxidant Capacity of fasting plasma and saliva among middle-aged and older subjects. AU - Gawron-Skarbek,Anna, AU - Prymont-Przymińska,Anna, AU - Sobczak,Agnieszka, AU - Guligowska,Agnieszka, AU - Kostka,Tomasz, AU - Nowak,Dariusz, AU - Szatko,Franciszek, Y1 - 2017/10/31/ PY - 2017/11/2/pubmed PY - 2018/8/7/medline PY - 2017/11/2/entrez KW - DPPH KW - FRAP KW - FRAS KW - Total antioxidant capacity KW - non-urate total antioxidant capacity KW - plasma KW - saliva KW - uric acid SP - 57 EP - 62 JF - Redox report : communications in free radical research JO - Redox Rep VL - 23 IS - 1 N2 - OBJECTIVES: As plasma and salivary total antioxidant capacity (TAC) is mainly contributed by uric acid (UA), the present study measures non-urate TAC (Nu-TAC). The aim of the study was to correlate plasma native TAC, Nu-TAC and UA with their salivary analogues, and compare the UA contribution in both body fluids using two different methods. METHODS: The study involved 55 middle-aged and older subjects (66.7 ± 4.5 years). TAC was determined simultaneously with two methods (ferric reducing ability of plasma - FRAP, 2.2-diphenyl-1-picryl-hydrazyl - DPPH and countertypes for saliva - FRAS and DPPHS test), with and without UA (native TAC and Nu-TAC, respectively). Plasma UA and salivary UA (SUA) were assessed. RESULTS: Subjects with increased FRAP, DPPH and UA had higher FRAS, DPPHS and SUA, respectively (P < 0.05). Plasma Nu-TAC indices did not correlate with salivary Nu-TAC. The contribution of UA to the plasma and salivary DPPH tests was similar: 75.7 ± 10.3% and 75.2 ± 14.0%, respectively. However, the contribution of UA to FRAS was higher than that for FRAP (71.6 ± 13.9% vs. 64.0 ± 8.1%; P < 0.001). DISCUSSION: Our findings suggest that saliva is a good predictor for native plasma TAC but not for Nu-TAC. UA level is comparably dominant in saliva and in plasma according to DPPH, but lower in plasma according to FRAP. SN - 1743-2928 UR - https://www.unboundmedicine.com/medline/citation/29088986/A_comparison_of_native_and_non_urate_Total_Antioxidant_Capacity_of_fasting_plasma_and_saliva_among_middle_aged_and_older_subjects_ DB - PRIME DP - Unbound Medicine ER -