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Genetic expansion of chaperonin-containing TCP-1 (CCT/TRiC) complex subunits yields testis-specific isoforms required for spermatogenesis in planarian flatworms.
Mol Reprod Dev. 2017 12; 84(12):1271-1284.MR

Abstract

Chaperonin-containing Tail-less complex polypeptide 1 (CCT) is a highly conserved, hetero-oligomeric complex that ensures proper folding of actin, tubulin, and regulators of mitosis. Eight subunits (CCT1-8) make up this complex, and every subunit has a homolog expressed in the testes and somatic tissue of the planarian flatworm Schmidtea mediterranea. Gene duplications of four subunits in the genomes of S. mediterranea and other planarian flatworms created paralogs to CCT1, CCT3, CCT4, and CCT8 that are expressed exclusively in the testes. Functional analyses revealed that each CCT subunit expressed in the S. mediterranea soma is essential for homeostatic integrity and survival, whereas sperm elongation defects were observed upon knockdown of each individual testis-specific paralog (Smed-cct1B; Smed-cct3B; Smed-cct4A; and Smed-cct8B), regardless of potential redundancy with paralogs expressed in both testes and soma (Smed-cct1A; Smed-cct3A; Smed-cct4B; and Smed-cct8A). Yet, no detriment was observed in the number of adult somatic stem cells (neoblasts) that maintain differentiated tissue in planarians. Thus, expression of all eight CCT subunits is required to execute the essential functions of the CCT complex. Furthermore, expression of the somatic paralogs in planarian testes is not sufficient to complete spermatogenesis when testis-specific paralogs are knocked down, suggesting that the evolution of chaperonin subunits may drive changes in the development of sperm structure and that correct CCT subunit stoichiometry is crucial for spermiogenesis.

Authors+Show Affiliations

Department of Biological Sciences, Wright State University, Dayton, Ohio.Department of Biological Sciences, Wright State University, Dayton, Ohio.Department of Biological Sciences, Wright State University, Dayton, Ohio.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

29095551

Citation

Counts, Jenna T., et al. "Genetic Expansion of Chaperonin-containing TCP-1 (CCT/TRiC) Complex Subunits Yields Testis-specific Isoforms Required for Spermatogenesis in Planarian Flatworms." Molecular Reproduction and Development, vol. 84, no. 12, 2017, pp. 1271-1284.
Counts JT, Hester TM, Rouhana L. Genetic expansion of chaperonin-containing TCP-1 (CCT/TRiC) complex subunits yields testis-specific isoforms required for spermatogenesis in planarian flatworms. Mol Reprod Dev. 2017;84(12):1271-1284.
Counts, J. T., Hester, T. M., & Rouhana, L. (2017). Genetic expansion of chaperonin-containing TCP-1 (CCT/TRiC) complex subunits yields testis-specific isoforms required for spermatogenesis in planarian flatworms. Molecular Reproduction and Development, 84(12), 1271-1284. https://doi.org/10.1002/mrd.22925
Counts JT, Hester TM, Rouhana L. Genetic Expansion of Chaperonin-containing TCP-1 (CCT/TRiC) Complex Subunits Yields Testis-specific Isoforms Required for Spermatogenesis in Planarian Flatworms. Mol Reprod Dev. 2017;84(12):1271-1284. PubMed PMID: 29095551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic expansion of chaperonin-containing TCP-1 (CCT/TRiC) complex subunits yields testis-specific isoforms required for spermatogenesis in planarian flatworms. AU - Counts,Jenna T, AU - Hester,Tasha M, AU - Rouhana,Labib, Y1 - 2017/11/10/ PY - 2017/08/01/received PY - 2017/10/16/accepted PY - 2017/11/3/pubmed PY - 2019/4/12/medline PY - 2017/11/3/entrez KW - TCP-1 ring complex (TRiC) KW - chaperonin-containing TCP-1 (CCT) complex KW - platyhelminthes KW - spermatogenesis KW - spermiogenesis SP - 1271 EP - 1284 JF - Molecular reproduction and development JO - Mol. Reprod. Dev. VL - 84 IS - 12 N2 - Chaperonin-containing Tail-less complex polypeptide 1 (CCT) is a highly conserved, hetero-oligomeric complex that ensures proper folding of actin, tubulin, and regulators of mitosis. Eight subunits (CCT1-8) make up this complex, and every subunit has a homolog expressed in the testes and somatic tissue of the planarian flatworm Schmidtea mediterranea. Gene duplications of four subunits in the genomes of S. mediterranea and other planarian flatworms created paralogs to CCT1, CCT3, CCT4, and CCT8 that are expressed exclusively in the testes. Functional analyses revealed that each CCT subunit expressed in the S. mediterranea soma is essential for homeostatic integrity and survival, whereas sperm elongation defects were observed upon knockdown of each individual testis-specific paralog (Smed-cct1B; Smed-cct3B; Smed-cct4A; and Smed-cct8B), regardless of potential redundancy with paralogs expressed in both testes and soma (Smed-cct1A; Smed-cct3A; Smed-cct4B; and Smed-cct8A). Yet, no detriment was observed in the number of adult somatic stem cells (neoblasts) that maintain differentiated tissue in planarians. Thus, expression of all eight CCT subunits is required to execute the essential functions of the CCT complex. Furthermore, expression of the somatic paralogs in planarian testes is not sufficient to complete spermatogenesis when testis-specific paralogs are knocked down, suggesting that the evolution of chaperonin subunits may drive changes in the development of sperm structure and that correct CCT subunit stoichiometry is crucial for spermiogenesis. SN - 1098-2795 UR - https://www.unboundmedicine.com/medline/citation/29095551/Genetic_expansion_of_chaperonin_containing_TCP_1__CCT/TRiC__complex_subunits_yields_testis_specific_isoforms_required_for_spermatogenesis_in_planarian_flatworms_ L2 - https://doi.org/10.1002/mrd.22925 DB - PRIME DP - Unbound Medicine ER -