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Genome-wide identification and expression analysis of glutathione S-transferase gene family in tomato: Gaining an insight to their physiological and stress-specific roles.
PLoS One. 2017; 12(11):e0187504.Plos

Abstract

Glutathione S-transferase (GST) refers to one of the major detoxifying enzymes that plays an important role in different abiotic and biotic stress modulation pathways of plant. The present study aimed to a comprehensive genome-wide functional characterization of GST genes and proteins in tomato (Solanum lycopersicum L.). The whole genome sequence analysis revealed the presence of 90 GST genes in tomato, the largest GST gene family reported till date. Eight segmental duplicated gene pairs might contribute significantly to the expansion of SlGST gene family. Based on phylogenetic analysis of tomato, rice, and Arabidopsis GST proteins, GST family members could be further divided into ten classes. Members of each orthologous class showed high conservancy among themselves. Tau and lambda are the major classes of tomato; while tau and phi are the major classes for rice and Arabidopsis. Chromosomal localization revealed highly uneven distribution of SlGST genes in 13 different chromosomes, where chromosome 9 possessed the highest number of genes. Based on publicly available microarray data, expression analysis of 30 available SlGST genes exhibited a differential pattern in all the analyzed tissues and developmental stages. Moreover, most of the members showed highly induced expression in response to multiple biotic and abiotic stress inducers that could be harmonized with the increase in total GST enzyme activity under several stress conditions. Activity of tomato GST could be enhanced further by using some positive modulators (safeners) that have been predicted through molecular docking of SlGSTU5 and ligands. Moreover, tomato GST proteins are predicted to interact with a lot of other glutathione synthesizing and utilizing enzymes such as glutathione peroxidase, glutathione reductase, glutathione synthetase and γ-glutamyltransferase. This comprehensive genome-wide analysis and expression profiling would provide a rational platform and possibility to explore the versatile role of GST genes in crop engineering.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka, Bangladesh.Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka, Bangladesh.Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29095889

Citation

Islam, Shiful, et al. "Genome-wide Identification and Expression Analysis of Glutathione S-transferase Gene Family in Tomato: Gaining an Insight to Their Physiological and Stress-specific Roles." PloS One, vol. 12, no. 11, 2017, pp. e0187504.
Islam S, Rahman IA, Islam T, et al. Genome-wide identification and expression analysis of glutathione S-transferase gene family in tomato: Gaining an insight to their physiological and stress-specific roles. PLoS One. 2017;12(11):e0187504.
Islam, S., Rahman, I. A., Islam, T., & Ghosh, A. (2017). Genome-wide identification and expression analysis of glutathione S-transferase gene family in tomato: Gaining an insight to their physiological and stress-specific roles. PloS One, 12(11), e0187504. https://doi.org/10.1371/journal.pone.0187504
Islam S, et al. Genome-wide Identification and Expression Analysis of Glutathione S-transferase Gene Family in Tomato: Gaining an Insight to Their Physiological and Stress-specific Roles. PLoS One. 2017;12(11):e0187504. PubMed PMID: 29095889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide identification and expression analysis of glutathione S-transferase gene family in tomato: Gaining an insight to their physiological and stress-specific roles. AU - Islam,Shiful, AU - Rahman,Iffat Ara, AU - Islam,Tahmina, AU - Ghosh,Ajit, Y1 - 2017/11/02/ PY - 2017/03/31/received PY - 2017/10/21/accepted PY - 2017/11/3/entrez PY - 2017/11/3/pubmed PY - 2017/11/29/medline SP - e0187504 EP - e0187504 JF - PloS one JO - PLoS One VL - 12 IS - 11 N2 - Glutathione S-transferase (GST) refers to one of the major detoxifying enzymes that plays an important role in different abiotic and biotic stress modulation pathways of plant. The present study aimed to a comprehensive genome-wide functional characterization of GST genes and proteins in tomato (Solanum lycopersicum L.). The whole genome sequence analysis revealed the presence of 90 GST genes in tomato, the largest GST gene family reported till date. Eight segmental duplicated gene pairs might contribute significantly to the expansion of SlGST gene family. Based on phylogenetic analysis of tomato, rice, and Arabidopsis GST proteins, GST family members could be further divided into ten classes. Members of each orthologous class showed high conservancy among themselves. Tau and lambda are the major classes of tomato; while tau and phi are the major classes for rice and Arabidopsis. Chromosomal localization revealed highly uneven distribution of SlGST genes in 13 different chromosomes, where chromosome 9 possessed the highest number of genes. Based on publicly available microarray data, expression analysis of 30 available SlGST genes exhibited a differential pattern in all the analyzed tissues and developmental stages. Moreover, most of the members showed highly induced expression in response to multiple biotic and abiotic stress inducers that could be harmonized with the increase in total GST enzyme activity under several stress conditions. Activity of tomato GST could be enhanced further by using some positive modulators (safeners) that have been predicted through molecular docking of SlGSTU5 and ligands. Moreover, tomato GST proteins are predicted to interact with a lot of other glutathione synthesizing and utilizing enzymes such as glutathione peroxidase, glutathione reductase, glutathione synthetase and γ-glutamyltransferase. This comprehensive genome-wide analysis and expression profiling would provide a rational platform and possibility to explore the versatile role of GST genes in crop engineering. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/29095889/Genome_wide_identification_and_expression_analysis_of_glutathione_S_transferase_gene_family_in_tomato:_Gaining_an_insight_to_their_physiological_and_stress_specific_roles_ L2 - https://dx.plos.org/10.1371/journal.pone.0187504 DB - PRIME DP - Unbound Medicine ER -