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Modification of the oxygen affinity and intracellular hemoglobin concentration of normal and sickle cells by means of an osmotic pulse.
J Lab Clin Med. 1989 Jan; 113(1):58-66.JL

Abstract

Important properties of red blood cells (RBCs), including oxygen affinity and intracellular hemoglobin concentration, may be modified by means of an osmotic pulse. The pulse is developed by rapid dilution of a suspension of RBCs to which dimethylsulfoxide has been added. The nature and degree of RBC modification is dependent on the composition of the diluent solution. The purpose of this investigation was to explore the dependence of these changes on diluent composition and to determine the stability of altered cellular properties with in vitro incubation. For both normal control RBCs (A cells) and RBCs from patients with sickle cell anemia (S cells), cells with increased volume and markedly decreased intracellular hemoglobin concentration (65% to 70% of control) or with normal volume and moderately decreased hemoglobin concentration (80% to 85% of control) were prepared. These modifications were accomplished with hemoglobin yields ranging from 60% to 90%, depending on the diluent used, the cell type, and the intensity of treatment. For the same degree of inositol hexaphosphate (IHP) incorporation into A cells, as shown by the shift in oxygen half-saturation pressure (P50), diluent formulations with constant total osmolality but varying IHP concentration gave the same degree of hemoglobin loss per cell. Posttreatment cell size, however, ranged from slightly smaller than control to approximately 20% larger. On incubation, the larger cells returned toward normal size, whereas those with normal posttreatment size remained constant. S cells showed the same general changes with treatment and incubation but with greater variation. The treated S cells exhibited markedly reduced morphologic sickling on deoxygenation.

Authors+Show Affiliations

Department of Internal Medicine, University of Cincinnati College of Medicine, OH.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2909651

Citation

Franco, R S., and R L. Barker. "Modification of the Oxygen Affinity and Intracellular Hemoglobin Concentration of Normal and Sickle Cells By Means of an Osmotic Pulse." The Journal of Laboratory and Clinical Medicine, vol. 113, no. 1, 1989, pp. 58-66.
Franco RS, Barker RL. Modification of the oxygen affinity and intracellular hemoglobin concentration of normal and sickle cells by means of an osmotic pulse. J Lab Clin Med. 1989;113(1):58-66.
Franco, R. S., & Barker, R. L. (1989). Modification of the oxygen affinity and intracellular hemoglobin concentration of normal and sickle cells by means of an osmotic pulse. The Journal of Laboratory and Clinical Medicine, 113(1), 58-66.
Franco RS, Barker RL. Modification of the Oxygen Affinity and Intracellular Hemoglobin Concentration of Normal and Sickle Cells By Means of an Osmotic Pulse. J Lab Clin Med. 1989;113(1):58-66. PubMed PMID: 2909651.
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TY - JOUR T1 - Modification of the oxygen affinity and intracellular hemoglobin concentration of normal and sickle cells by means of an osmotic pulse. AU - Franco,R S, AU - Barker,R L, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 58 EP - 66 JF - The Journal of laboratory and clinical medicine JO - J Lab Clin Med VL - 113 IS - 1 N2 - Important properties of red blood cells (RBCs), including oxygen affinity and intracellular hemoglobin concentration, may be modified by means of an osmotic pulse. The pulse is developed by rapid dilution of a suspension of RBCs to which dimethylsulfoxide has been added. The nature and degree of RBC modification is dependent on the composition of the diluent solution. The purpose of this investigation was to explore the dependence of these changes on diluent composition and to determine the stability of altered cellular properties with in vitro incubation. For both normal control RBCs (A cells) and RBCs from patients with sickle cell anemia (S cells), cells with increased volume and markedly decreased intracellular hemoglobin concentration (65% to 70% of control) or with normal volume and moderately decreased hemoglobin concentration (80% to 85% of control) were prepared. These modifications were accomplished with hemoglobin yields ranging from 60% to 90%, depending on the diluent used, the cell type, and the intensity of treatment. For the same degree of inositol hexaphosphate (IHP) incorporation into A cells, as shown by the shift in oxygen half-saturation pressure (P50), diluent formulations with constant total osmolality but varying IHP concentration gave the same degree of hemoglobin loss per cell. Posttreatment cell size, however, ranged from slightly smaller than control to approximately 20% larger. On incubation, the larger cells returned toward normal size, whereas those with normal posttreatment size remained constant. S cells showed the same general changes with treatment and incubation but with greater variation. The treated S cells exhibited markedly reduced morphologic sickling on deoxygenation. SN - 0022-2143 UR - https://www.unboundmedicine.com/medline/citation/2909651/Modification_of_the_oxygen_affinity_and_intracellular_hemoglobin_concentration_of_normal_and_sickle_cells_by_means_of_an_osmotic_pulse_ L2 - https://antibodies.cancer.gov/detail/CPTC-CD40-1 DB - PRIME DP - Unbound Medicine ER -