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Chemical characterization and cytotoxic, genotoxic, and mutagenic properties of Baccharis trinervis (Lam, Persoon) from Colombia and Brazil.
J Ethnopharmacol. 2018 Mar 01; 213:210-220.JE

Abstract

PHARMACOLOGY RELEVANCE

Baccharis trinervis (Lam, Persoon) leaves are used in the traditional medicine for the treatment of high fevers, edema, inflammation, sores and muscle cramps, snakebites and as antiseptic.

AIM OF THE STUDY

To investigate the cytotoxic, genotoxic, and mutagenic effects of extracts and fractions of B. trinervis from Brazil and Colombia in Chinese Hamster Ovary (CHO) cells, and to examine the mutagenic activity in Salmonella typhimurium.

MATERIAL AND METHODS

Aqueous extracts (AE) of aerial parts of B. trinervis from Brazil (B) and Colombia (C) were fractioned in ethyl acetate fraction (EAF), butanol extract (BF), and aqueous residue fraction (ARF). Qualitative chemical screening and determination of total flavonoid content were made. Identification of chemical constituents was performed by High Performance Liquid Chromatography (HPLC) and High Resolution Mass Spectrometry (HRMS). For the in vitro tests, CHO cells were treated for 3h with extracts and fractions. The cytotoxic activity was evaluated by clonal survival and 3-(4.5-dimethylthiazole-2-yl)-2.5-biphenyl tetrazolium bromide reduction assay (MTT). Genotoxic and mutagenic effects were evaluated by the alkaline comet assay and Cytokinesis-blockage micronucleus test (CBMN), respectively. Additionally, Salmonella/microsome assay was carried out to determinate the mutagenic effects in EAF from Brazil and Colombia.

RESULTS

Phytochemical analyses indicated the presence of saponins and flavonoids. AE and EAF were the samples with the highest quantity of total flavonoids. HPLC showed the presence of luteolin only in AEC, and caffeic acid, ellagic acid, rosmarinic acid, and rutin were identified in AEB and AEC (AEC>AEB). The HRMS in positive mode of EAFB and EAFC showed presence of two carboxylic acids, coumarin, and two terpenoids. In addition, were identified one terpenoid and two carboxylic acids in AE, BF and ARF of B. trinervis from both countries in negative mode. Dose-dependent cytotoxic effects were observed in CHO cells treated with B. trinervis extracts and fractions by using clonal survival and MTT at concentrations higher than 0.05mg/mL. All the extracts and fractions induced DNA strand breaks in CHO cells with dose-dependent response, mostly EAFB and EAFC. The EAF from Brazil and Colombia showed mutagenic effect at 0.5mg/mL, while the other fractions did not show a significant difference in relation to the control. No mutagenic effects were found in EAF from both countries by the Salmonella/microsome assay.

CONCLUSIONS

Cytotoxic and genotoxic effects were demonstrated in all extracts and fractions used, although only EAF showed mutagenic effects by CBMN, but not by Salmonella/microsome assay. Our results suggest that flavonoids, phenylpropanoids, coumarins, and diterpenes may be responsible for the cytotoxic, genotoxic and mutagenic effects observed.

Authors+Show Affiliations

Departamento de Biofísica/Centro de Biotecnologia-UFRGS, Porto Alegre- RS-Brasil.; Programa de Pós Graduação em Biologia Celular e Molecular (PPGBCM), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.Unidad de Investigación, Desarrollo e Innovación en Genética y Biología Molecular, Universidad Simón Bolívar, Barranquilla, Colombia.Programa de Pós Graduação em Biologia Celular e Molecular Aplicada à Saúde - ULBRA, Canoas, RS, Brazil.Laboratório de Cardiologia Celular e Molecular, PPG em Ciências da Saúde: Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Porto Alegre, Brazil.InnVitro Research and Development, Av. Osvaldo Aranha, 1022 sl 1415, 90035-190 Porto Alegre, RS, Brazil.Laboratorio de Epigenética e Inestabilidad Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.Programa de Pós Graduação em Biologia Celular e Molecular Aplicada à Saúde - ULBRA, Canoas, RS, Brazil.Programa de Pós Graduação em Biologia Celular e Molecular Aplicada à Saúde - ULBRA, Canoas, RS, Brazil.Centro de Ciências Exatas e de Tecnologia, Instituto de Biotecnologia, Universidade de Caxias do Sul - UCS Caxias do Sul - RS, Brazil.Unidad de Investigación, Desarrollo e Innovación en Genética y Biología Molecular, Universidad Simón Bolívar, Barranquilla, Colombia.Programa de Pós Graduação em Biologia Celular e Molecular Aplicada à Saúde - ULBRA, Canoas, RS, Brazil.Departamento de Biofísica/Centro de Biotecnologia-UFRGS, Porto Alegre- RS-Brasil.; Programa de Pós Graduação em Biologia Celular e Molecular (PPGBCM), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Centro de Ciências Exatas e de Tecnologia, Instituto de Biotecnologia, Universidade de Caxias do Sul - UCS Caxias do Sul - RS, Brazil. Electronic address: pegas.henriques@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29100934

Citation

Jaramillo-García, Victoria, et al. "Chemical Characterization and Cytotoxic, Genotoxic, and Mutagenic Properties of Baccharis Trinervis (Lam, Persoon) From Colombia and Brazil." Journal of Ethnopharmacology, vol. 213, 2018, pp. 210-220.
Jaramillo-García V, Trindade C, Lima E, et al. Chemical characterization and cytotoxic, genotoxic, and mutagenic properties of Baccharis trinervis (Lam, Persoon) from Colombia and Brazil. J Ethnopharmacol. 2018;213:210-220.
Jaramillo-García, V., Trindade, C., Lima, E., Guecheva, T. N., Villela, I., Martinez-Lopez, W., Corrêa, D. S., Ferraz, A. B. F., Moura, S., Sosa, M. Q., Da Silva, J., & Henriques, J. A. P. (2018). Chemical characterization and cytotoxic, genotoxic, and mutagenic properties of Baccharis trinervis (Lam, Persoon) from Colombia and Brazil. Journal of Ethnopharmacology, 213, 210-220. https://doi.org/10.1016/j.jep.2017.10.027
Jaramillo-García V, et al. Chemical Characterization and Cytotoxic, Genotoxic, and Mutagenic Properties of Baccharis Trinervis (Lam, Persoon) From Colombia and Brazil. J Ethnopharmacol. 2018 Mar 1;213:210-220. PubMed PMID: 29100934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical characterization and cytotoxic, genotoxic, and mutagenic properties of Baccharis trinervis (Lam, Persoon) from Colombia and Brazil. AU - Jaramillo-García,Victoria, AU - Trindade,Cristiano, AU - Lima,Elisiane, AU - Guecheva,Temenouga N, AU - Villela,Izabel, AU - Martinez-Lopez,Wilner, AU - Corrêa,Dione S, AU - Ferraz,Alexandre de B F, AU - Moura,Sidnei, AU - Sosa,Milton Quintana, AU - Da Silva,Juliana, AU - Henriques,João Antônio Pegas, Y1 - 2017/11/01/ PY - 2017/07/31/received PY - 2017/10/25/revised PY - 2017/10/29/accepted PY - 2017/11/5/pubmed PY - 2018/8/14/medline PY - 2017/11/5/entrez KW - Baccharis trinervis KW - Chemical composition KW - Cytotoxicity KW - Genotoxicity KW - Mutagenicity SP - 210 EP - 220 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 213 N2 - PHARMACOLOGY RELEVANCE: Baccharis trinervis (Lam, Persoon) leaves are used in the traditional medicine for the treatment of high fevers, edema, inflammation, sores and muscle cramps, snakebites and as antiseptic. AIM OF THE STUDY: To investigate the cytotoxic, genotoxic, and mutagenic effects of extracts and fractions of B. trinervis from Brazil and Colombia in Chinese Hamster Ovary (CHO) cells, and to examine the mutagenic activity in Salmonella typhimurium. MATERIAL AND METHODS: Aqueous extracts (AE) of aerial parts of B. trinervis from Brazil (B) and Colombia (C) were fractioned in ethyl acetate fraction (EAF), butanol extract (BF), and aqueous residue fraction (ARF). Qualitative chemical screening and determination of total flavonoid content were made. Identification of chemical constituents was performed by High Performance Liquid Chromatography (HPLC) and High Resolution Mass Spectrometry (HRMS). For the in vitro tests, CHO cells were treated for 3h with extracts and fractions. The cytotoxic activity was evaluated by clonal survival and 3-(4.5-dimethylthiazole-2-yl)-2.5-biphenyl tetrazolium bromide reduction assay (MTT). Genotoxic and mutagenic effects were evaluated by the alkaline comet assay and Cytokinesis-blockage micronucleus test (CBMN), respectively. Additionally, Salmonella/microsome assay was carried out to determinate the mutagenic effects in EAF from Brazil and Colombia. RESULTS: Phytochemical analyses indicated the presence of saponins and flavonoids. AE and EAF were the samples with the highest quantity of total flavonoids. HPLC showed the presence of luteolin only in AEC, and caffeic acid, ellagic acid, rosmarinic acid, and rutin were identified in AEB and AEC (AEC>AEB). The HRMS in positive mode of EAFB and EAFC showed presence of two carboxylic acids, coumarin, and two terpenoids. In addition, were identified one terpenoid and two carboxylic acids in AE, BF and ARF of B. trinervis from both countries in negative mode. Dose-dependent cytotoxic effects were observed in CHO cells treated with B. trinervis extracts and fractions by using clonal survival and MTT at concentrations higher than 0.05mg/mL. All the extracts and fractions induced DNA strand breaks in CHO cells with dose-dependent response, mostly EAFB and EAFC. The EAF from Brazil and Colombia showed mutagenic effect at 0.5mg/mL, while the other fractions did not show a significant difference in relation to the control. No mutagenic effects were found in EAF from both countries by the Salmonella/microsome assay. CONCLUSIONS: Cytotoxic and genotoxic effects were demonstrated in all extracts and fractions used, although only EAF showed mutagenic effects by CBMN, but not by Salmonella/microsome assay. Our results suggest that flavonoids, phenylpropanoids, coumarins, and diterpenes may be responsible for the cytotoxic, genotoxic and mutagenic effects observed. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/29100934/Chemical_characterization_and_cytotoxic_genotoxic_and_mutagenic_properties_of_Baccharis_trinervis__Lam_Persoon__from_Colombia_and_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(17)32873-8 DB - PRIME DP - Unbound Medicine ER -