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Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat.
Theor Appl Genet. 2018 Feb; 131(2):389-406.TA

Abstract

KEY MESSAGE

Genome-wide introgressions of Thinopyrum bessarabicum into wheat resulted in 12 recombinant lines. Cytological and molecular techniques allowed mapping of 1150 SNP markers across all seven chromosomes of the J genome. Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important source for new genetic variation for wheat improvement due to its salinity tolerance and disease resistance. Its practical utilisation in wheat improvement can be facilitated through development of genome-wide introgressions leading to a variety of different wheat-Th . bessarabicum translocation lines. In this study, we report the generation of 12 such wheat-Th . bessarabicum recombinant lines, through two different crossing strategies, which were characterized using sequential single colour and multi-colour genomic in situ hybridization (sc-GISH and mc-GISH), multi-colour fluorescent in situ hybridization (mc-FISH) and single nucleotide polymorphic (SNP) DNA markers. We also detected 13 lines containing different Th. bessarabicum chromosome aberrations through sc-GISH. Through a combination of molecular and cytological analysis of all the 25 lines containing Th. bessarabicum recombinants and chromosome aberrations we were able to physically map 1150 SNP markers onto seven Th. bessarabicum J chromosomes which were divided into 36 segmental blocks. Comparative analysis of the physical map of Th. bessarabicum and the wheat genome showed that synteny between the two species is highly conserved at the macro-level and confirmed that Th. bessarabicum contains the 4/5 translocation also present in the A genome of wheat. These wheat-Th . bessarabicum recombinant lines and SNP markers provide a useful genetic resource for wheat improvement with the latter having a wider impact as a tool for detection of introgressions from other Thinopyrum species containing the J or a closely-related genome such as Thinopyrum intermedium (JrJrJvsJvsStSt) and Thinopyrum elongatum (EeEe), respectively.

Links

Authors+Show Affiliations

Nottingham/BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK. surbhi.grewal@nottingham.ac.uk.

Nottingham/BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.

Nottingham/BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.

Nottingham/BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.

Nottingham/BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.

Life Sciences, University of Bristol, Bristol, BS8 1TQ, UK.

Nottingham/BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.

Nottingham/BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.

MeSH

Chromosome MappingChromosomes, PlantGenetic MarkersHybridization, GeneticIn Situ Hybridization, FluorescencePoaceaePolymorphism, Single NucleotideSyntenyTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29101420

Citation

Grewal, Surbhi, et al. "Characterisation of Thinopyrum Bessarabicum Chromosomes Through Genome-wide Introgressions Into Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 2, 2018, pp. 389-406.

Grewal S, Yang C, Edwards SH, et al. Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat. Theor Appl Genet. 2018;131(2):389-406.

Grewal, S., Yang, C., Edwards, S. H., Scholefield, D., Ashling, S., Burridge, A. J., King, I. P., & King, J. (2018). Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(2), 389-406. https://doi.org/10.1007/s00122-017-3009-y

Grewal S, et al. Characterisation of Thinopyrum Bessarabicum Chromosomes Through Genome-wide Introgressions Into Wheat. Theor Appl Genet. 2018;131(2):389-406. PubMed PMID: 29101420.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat. AU - Grewal,Surbhi, AU - Yang,Caiyun, AU - Edwards,Stella Hubbart, AU - Scholefield,Duncan, AU - Ashling,Stephen, AU - Burridge,Amanda J, AU - King,Ian P, AU - King,Julie, Y1 - 2017/11/03/ PY - 2017/08/18/received PY - 2017/10/26/accepted PY - 2017/11/5/pubmed PY - 2018/4/20/medline PY - 2017/11/5/entrez SP - 389 EP - 406 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 2 N2 - KEY MESSAGE: Genome-wide introgressions of Thinopyrum bessarabicum into wheat resulted in 12 recombinant lines. Cytological and molecular techniques allowed mapping of 1150 SNP markers across all seven chromosomes of the J genome. Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important source for new genetic variation for wheat improvement due to its salinity tolerance and disease resistance. Its practical utilisation in wheat improvement can be facilitated through development of genome-wide introgressions leading to a variety of different wheat-Th . bessarabicum translocation lines. In this study, we report the generation of 12 such wheat-Th . bessarabicum recombinant lines, through two different crossing strategies, which were characterized using sequential single colour and multi-colour genomic in situ hybridization (sc-GISH and mc-GISH), multi-colour fluorescent in situ hybridization (mc-FISH) and single nucleotide polymorphic (SNP) DNA markers. We also detected 13 lines containing different Th. bessarabicum chromosome aberrations through sc-GISH. Through a combination of molecular and cytological analysis of all the 25 lines containing Th. bessarabicum recombinants and chromosome aberrations we were able to physically map 1150 SNP markers onto seven Th. bessarabicum J chromosomes which were divided into 36 segmental blocks. Comparative analysis of the physical map of Th. bessarabicum and the wheat genome showed that synteny between the two species is highly conserved at the macro-level and confirmed that Th. bessarabicum contains the 4/5 translocation also present in the A genome of wheat. These wheat-Th . bessarabicum recombinant lines and SNP markers provide a useful genetic resource for wheat improvement with the latter having a wider impact as a tool for detection of introgressions from other Thinopyrum species containing the J or a closely-related genome such as Thinopyrum intermedium (JrJrJvsJvsStSt) and Thinopyrum elongatum (EeEe), respectively. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29101420/Characterisation_of_Thinopyrum_bessarabicum_chromosomes_through_genome_wide_introgressions_into_wheat_ DB - PRIME DP - Unbound Medicine ER -