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Simultaneous quantification of five biflavonoids in rat plasma by LC-ESI-MS/MS and its application to a comparatively pharmacokinetic study of Selaginella doederleinii Hieron extract in rats.
J Pharm Biomed Anal 2018; 149:80-88JP

Abstract

Selaginella doederleinii Hieron is a widely used as folk Chinese medicine for treatment of different cancers. Our previous investigations have confirmed that the total biflavonoids in ethyl acetate extract from S. doederleinii (SDEA) have favorable anticancer potentials. However, the in vivo process of its bioactive ingredients remains unknown. In this paper, a sensitive and reliable method was developed for simultaneous quantification of main five biflavonoids, including amentoflavone, robustaflavone, 2″,3″-dihydro-3',3″-biapigenin, 3',3″-binaringenin and delicaflavone in the ethyl acetate extract of S. doederleinii (SDEA extract) in rat plasma by high-performance liquid chromatography with electrospray ionization-mass spectrometry (HPLC-ESI-MS/MS). Chromatographic separation was performed using an Ultimate® XB-C18 (100×2.1mm, 3.5μm) with gradient elution of water (0.5% acetic acid) and acetonitrile at 0.2mL/min. All analytes with internal standard (chrysin) were detected using selective reaction monitoring (SRM) in negative ionization mode. The method showed a good linearity over a wide concentration range (r2>0.99). The limits of quantification for the biflavonoids were less than 10ng/mL. The developed method was applied to the comparatively pharmacokinetic study of the five biflavonoids after oral or intravenous administration of SDEA extract in rats. In addition, in silico assessments of permeability and solubility of these biflavonoids were also performed to understand their poor bioavailability. It is the first time to report the in vivo process profiles of the biflavonoids of SDEA extract in rats.

Authors+Show Affiliations

Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.Institute of Immunotherapy, Fujian Medical University, Fuzhou 350122, China.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.Department of Traditional Chinese Medicine Resource and Bee Products, College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. Electronic address: xhlin1963@sina.com.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. Electronic address: yauhung@126.com.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

29101819

Citation

Chen, Bing, et al. "Simultaneous Quantification of Five Biflavonoids in Rat Plasma By LC-ESI-MS/MS and Its Application to a Comparatively Pharmacokinetic Study of Selaginella Doederleinii Hieron Extract in Rats." Journal of Pharmaceutical and Biomedical Analysis, vol. 149, 2018, pp. 80-88.
Chen B, Wang X, Zou Y, et al. Simultaneous quantification of five biflavonoids in rat plasma by LC-ESI-MS/MS and its application to a comparatively pharmacokinetic study of Selaginella doederleinii Hieron extract in rats. J Pharm Biomed Anal. 2018;149:80-88.
Chen, B., Wang, X., Zou, Y., Chen, W., Wang, G., Yao, W., ... Yao, H. (2018). Simultaneous quantification of five biflavonoids in rat plasma by LC-ESI-MS/MS and its application to a comparatively pharmacokinetic study of Selaginella doederleinii Hieron extract in rats. Journal of Pharmaceutical and Biomedical Analysis, 149, pp. 80-88. doi:10.1016/j.jpba.2017.10.028.
Chen B, et al. Simultaneous Quantification of Five Biflavonoids in Rat Plasma By LC-ESI-MS/MS and Its Application to a Comparatively Pharmacokinetic Study of Selaginella Doederleinii Hieron Extract in Rats. J Pharm Biomed Anal. 2018 Feb 5;149:80-88. PubMed PMID: 29101819.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of five biflavonoids in rat plasma by LC-ESI-MS/MS and its application to a comparatively pharmacokinetic study of Selaginella doederleinii Hieron extract in rats. AU - Chen,Bing, AU - Wang,Xuewen, AU - Zou,Yulian, AU - Chen,Weiying, AU - Wang,Gang, AU - Yao,Wensong, AU - Shi,Peiying, AU - Li,Shaoguang, AU - Lin,Shilan, AU - Lin,Xinhua, AU - Yao,Hong, Y1 - 2017/10/24/ PY - 2017/08/01/received PY - 2017/10/04/revised PY - 2017/10/23/accepted PY - 2017/11/5/pubmed PY - 2018/7/28/medline PY - 2017/11/5/entrez KW - Biflavonoids KW - Bioavailability KW - HPLC–MS/MS KW - Pharmacokinetics KW - Selaginella doederleinii hieron SP - 80 EP - 88 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 149 N2 - Selaginella doederleinii Hieron is a widely used as folk Chinese medicine for treatment of different cancers. Our previous investigations have confirmed that the total biflavonoids in ethyl acetate extract from S. doederleinii (SDEA) have favorable anticancer potentials. However, the in vivo process of its bioactive ingredients remains unknown. In this paper, a sensitive and reliable method was developed for simultaneous quantification of main five biflavonoids, including amentoflavone, robustaflavone, 2″,3″-dihydro-3',3″-biapigenin, 3',3″-binaringenin and delicaflavone in the ethyl acetate extract of S. doederleinii (SDEA extract) in rat plasma by high-performance liquid chromatography with electrospray ionization-mass spectrometry (HPLC-ESI-MS/MS). Chromatographic separation was performed using an Ultimate® XB-C18 (100×2.1mm, 3.5μm) with gradient elution of water (0.5% acetic acid) and acetonitrile at 0.2mL/min. All analytes with internal standard (chrysin) were detected using selective reaction monitoring (SRM) in negative ionization mode. The method showed a good linearity over a wide concentration range (r2>0.99). The limits of quantification for the biflavonoids were less than 10ng/mL. The developed method was applied to the comparatively pharmacokinetic study of the five biflavonoids after oral or intravenous administration of SDEA extract in rats. In addition, in silico assessments of permeability and solubility of these biflavonoids were also performed to understand their poor bioavailability. It is the first time to report the in vivo process profiles of the biflavonoids of SDEA extract in rats. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/29101819/Simultaneous_quantification_of_five_biflavonoids_in_rat_plasma_by_LC_ESI_MS/MS_and_its_application_to_a_comparatively_pharmacokinetic_study_of_Selaginella_doederleinii_Hieron_extract_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(17)31941-6 DB - PRIME DP - Unbound Medicine ER -