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The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide.
Asian-Australas J Anim Sci. 2018 Apr; 31(4):548-555.AJ

Abstract

OBJECTIVE

This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in weaning pigs when challenged with lipopolysaccharide.

METHODS

Forty-eight weaned pigs (Duroc×Large White×Landrace, 8.12±0.56 kg) were assigned to four treatments: i) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; ii) lipopolysaccharide challenged control (LPSCC), piglets received the same control diet and injected with Escherichia coli LPS; iii) lipopolysaccharide (LPS)+0.5% Asn, piglets received a 0.5% Asn diet and injected with LPS; and iv) LPS+1.0% Asn, piglets received a 1.0% Asn diet and injected with LPS. All piglets were fed the experimental diets for 19 d. On d 20, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weights or the same volume of 0.9% NaCl solution based on the assigned treatments. Then the pigs were slaughtered at 4 h and 24 h after LPS or saline injection, and the liver samples were collected.

RESULTS

At 24 h after LPS challenge, dietary supplementation with 0.5% Asn increased ATP concentration (quadratic, p<0.05), and had a tendency to increase adenylate energy charges and reduce AMP/ATP ratio (quadratic, p<0.1) in liver. In addition, Asn increased the liver mRNA expression of pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase β (linear, p<0.05; quadratic, p<0.05), and had a tendency to increase the mRNA expression of hexokinase 2 (linear, p<0.1). Moreover, Asn increased liver phosphorylated AMP-activated protein kinase (pAMPK)/total AMP-activated protein kinase (tAMPK) ratio (linear, p<0.05; quadratic, p<0.05). However, at 4 h after LPS challenge, Asn supplementation had no effect on these parameters.

CONCLUSION

The present study indicated that Asn could improve the energy metabolism in injured liver at the late stage of LPS challenge.

Authors+Show Affiliations

Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29103285

Citation

Kang, Ping, et al. "The Effect of Dietary Asparagine Supplementation On Energy Metabolism in Liver of Weaning Pigs when Challenged With Lipopolysaccharide." Asian-Australasian Journal of Animal Sciences, vol. 31, no. 4, 2018, pp. 548-555.
Kang P, Liu Y, Zhu H, et al. The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide. Asian-Australasian journal of animal sciences. 2018;31(4):548-555.
Kang, P., Liu, Y., Zhu, H., Zhang, J., Shi, H., Li, S., Pi, D., Leng, W., Wang, X., Wu, H., & Hou, Y. (2018). The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide. Asian-Australasian Journal of Animal Sciences, 31(4), 548-555. https://doi.org/10.5713/ajas.17.0426
Kang P, et al. The Effect of Dietary Asparagine Supplementation On Energy Metabolism in Liver of Weaning Pigs when Challenged With Lipopolysaccharide. Asian-Australasian journal of animal sciences. 2018;31(4):548-555. PubMed PMID: 29103285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide. AU - Kang,Ping, AU - Liu,Yulan, AU - Zhu,Huiling, AU - Zhang,Jing, AU - Shi,Haifeng, AU - Li,Shuang, AU - Pi,Dinan, AU - Leng,Weibo, AU - Wang,Xiuying, AU - Wu,Huanting, AU - Hou,Yongqing, Y1 - 2017/11/03/ PY - 2017/06/03/received PY - 2017/09/08/revised PY - 2017/10/22/accepted PY - 2017/11/7/pubmed PY - 2017/11/7/medline PY - 2017/11/7/entrez KW - Asparagine KW - Energy Metabolism KW - Lipopolysaccharide KW - Piglets SP - 548 EP - 555 JF - Asian-Australasian journal of animal sciences VL - 31 IS - 4 N2 - OBJECTIVE: This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in weaning pigs when challenged with lipopolysaccharide. METHODS: Forty-eight weaned pigs (Duroc×Large White×Landrace, 8.12±0.56 kg) were assigned to four treatments: i) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; ii) lipopolysaccharide challenged control (LPSCC), piglets received the same control diet and injected with Escherichia coli LPS; iii) lipopolysaccharide (LPS)+0.5% Asn, piglets received a 0.5% Asn diet and injected with LPS; and iv) LPS+1.0% Asn, piglets received a 1.0% Asn diet and injected with LPS. All piglets were fed the experimental diets for 19 d. On d 20, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weights or the same volume of 0.9% NaCl solution based on the assigned treatments. Then the pigs were slaughtered at 4 h and 24 h after LPS or saline injection, and the liver samples were collected. RESULTS: At 24 h after LPS challenge, dietary supplementation with 0.5% Asn increased ATP concentration (quadratic, p<0.05), and had a tendency to increase adenylate energy charges and reduce AMP/ATP ratio (quadratic, p<0.1) in liver. In addition, Asn increased the liver mRNA expression of pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase β (linear, p<0.05; quadratic, p<0.05), and had a tendency to increase the mRNA expression of hexokinase 2 (linear, p<0.1). Moreover, Asn increased liver phosphorylated AMP-activated protein kinase (pAMPK)/total AMP-activated protein kinase (tAMPK) ratio (linear, p<0.05; quadratic, p<0.05). However, at 4 h after LPS challenge, Asn supplementation had no effect on these parameters. CONCLUSION: The present study indicated that Asn could improve the energy metabolism in injured liver at the late stage of LPS challenge. SN - 1011-2367 UR - https://www.unboundmedicine.com/medline/citation/29103285/The_effect_of_dietary_asparagine_supplementation_on_energy_metabolism_in_liver_of_weaning_pigs_when_challenged_with_lipopolysaccharide_ L2 - https://dx.doi.org/10.5713/ajas.17.0426 DB - PRIME DP - Unbound Medicine ER -
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