Tags

Type your tag names separated by a space and hit enter

[Effect of Acupuncture Stimulation of Different Acupoint Combinations on Sleep and Expression of Circadian Clock and Bmal 1 Genes in Hypothalamus of Insomnia Rats].
Zhen Ci Yan Jiu. 2017 Oct 25; 42(5):429-33.ZC

Abstract

OBJECTIVE

To observe the effect of manual acupuncture stimulation (MAS) of "Baihui"(GV 20)-"Shenmen"(HT 7)or GV 20-"Sanyinjiao"(SP 6) on sleep and expression of circadian rhythm genes (Clock and Bmal 1) in the hypothalamus in insomnia rats, so as to select a better acupoint group for insomnia.

METHODS

A total of 53 SD rats were randomly divided into normal control (n=12),insomnia model (n=8), GV 20-HT 7(n=12), GV 20-SP 6(n=11),and GV 20-non-acupoint (n=10) groups. The insomnia model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA,500 mg/kg,100 mg/mL) once daily for 2 days. The MAS (uniform reinforcing-reducing needling) was applied to GV 20-HT 7, GV 20-SP 6 or GV 20-non-acupoint for 30 min,once daily for 7 days. The sleep onset latency and sleep duration were gauged after intraperitoneal injection of pentobarbital sodium (35 mg/kg). The expression levels of Clock mRNA and Bmal 1 mRNA in the hypothalamic tissues containing ventrolateral preoptic area (VLPO) and suprachiasmatic nucleus (SCN) region were detected by fluorescence quantitative real-time PCR.

RESULTS

Following administration of pentobarbital sodium,the sleep latency was significantly prolonged and the sleep duration was considerably shortened in rats of the model group(P<0.05). After the treatment, the increased sleep latencies in the GV 20-HT 7, GV 20-SP 6 and GV 20-non-acupoints were all significantly down-regulated (P<0.05), and the decreased sleep duration was significantly increased only in the GV 20-HT 7 group relevant to the model group (P<0.05), but not in the GV 20-SP 6 and GV 20-non-acupoint groups (P<0.05). There were no significant differences in the sleep latency among the 3 treatment groups (P<0.05). The sleep duration was obviously prolonged in the GV 20-HT 7 group than in the GV 20-SP 6 and GV 20-non-acupoint groups (P<0.05). After modeling, the expression levels of Clock mRNA and Bmal 1 mRNA in hypothalamic VLPO and SCN regions were significantly down-regulated relevant to the normal control group (P<0.01). Following the treatment, the expression levels of Clock mRNA in the VLPO and SCN regions of the GV 20-SP 6 and GV 20-HT 7 groups, and those of Bmal 1 mRNA in the VLPO and SCN regions of the 3 treatment groups were considerably increased relevant to the model group (P<0.05, P<0.01). The effects of GV 20-HT 7 were significantly superior to those of GV 20-SP 6 and GV 20-non-acupoint (and also the action of GV 20-SP 6 was evidently superior to that of GV 20-non-acupoint) in up-regulating the expression of Clock mRNA and Bmal 1 mRNA in both VLPO and SCN regions (P<0.05, P<0.01).

CONCLUSIONS

Manual acupuncture stimulation of GV 20-HT 7 can improve the sleep latency and duration in insomnia rats,which may be associated with its effects in up-regulating the expression levels of circadian Clock mRNA and Bmal 1 mRNA in hypothalamic VLPO and SCN regions, and the efficacy of GV 20-HT 7 is obviously better than that of GV 20-SP 6 and GV 20-non-acupoint.

Authors+Show Affiliations

The First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. 624755064@qq.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29105472

Citation

Wei, Xin-Ran, et al. "[Effect of Acupuncture Stimulation of Different Acupoint Combinations On Sleep and Expression of Circadian Clock and Bmal 1 Genes in Hypothalamus of Insomnia Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 42, no. 5, 2017, pp. 429-33.
Wei XR, Wei GW, Zheng XN, et al. [Effect of Acupuncture Stimulation of Different Acupoint Combinations on Sleep and Expression of Circadian Clock and Bmal 1 Genes in Hypothalamus of Insomnia Rats]. Zhen Ci Yan Jiu. 2017;42(5):429-33.
Wei, X. R., Wei, G. W., Zheng, X. N., Wu, X. F., Chen, X. L., Liu, L., Wen, Q. Q., Xie, L. N., Xie, Z. Q., & Yue, Z. H. (2017). [Effect of Acupuncture Stimulation of Different Acupoint Combinations on Sleep and Expression of Circadian Clock and Bmal 1 Genes in Hypothalamus of Insomnia Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 42(5), 429-33.
Wei XR, et al. [Effect of Acupuncture Stimulation of Different Acupoint Combinations On Sleep and Expression of Circadian Clock and Bmal 1 Genes in Hypothalamus of Insomnia Rats]. Zhen Ci Yan Jiu. 2017 Oct 25;42(5):429-33. PubMed PMID: 29105472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Acupuncture Stimulation of Different Acupoint Combinations on Sleep and Expression of Circadian Clock and Bmal 1 Genes in Hypothalamus of Insomnia Rats]. AU - Wei,Xin-Ran, AU - Wei,Gao-Wen, AU - Zheng,Xue-Na, AU - Wu,Xue-Fen, AU - Chen,Xiao-Li, AU - Liu,Li, AU - Wen,Qi-Qi, AU - Xie,Li-Na, AU - Xie,Zhi-Qiang, AU - Yue,Zeng-Hui, PY - 2017/11/7/entrez PY - 2017/11/7/pubmed PY - 2019/4/4/medline SP - 429 EP - 33 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 42 IS - 5 N2 - OBJECTIVE: To observe the effect of manual acupuncture stimulation (MAS) of "Baihui"(GV 20)-"Shenmen"(HT 7)or GV 20-"Sanyinjiao"(SP 6) on sleep and expression of circadian rhythm genes (Clock and Bmal 1) in the hypothalamus in insomnia rats, so as to select a better acupoint group for insomnia. METHODS: A total of 53 SD rats were randomly divided into normal control (n=12),insomnia model (n=8), GV 20-HT 7(n=12), GV 20-SP 6(n=11),and GV 20-non-acupoint (n=10) groups. The insomnia model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA,500 mg/kg,100 mg/mL) once daily for 2 days. The MAS (uniform reinforcing-reducing needling) was applied to GV 20-HT 7, GV 20-SP 6 or GV 20-non-acupoint for 30 min,once daily for 7 days. The sleep onset latency and sleep duration were gauged after intraperitoneal injection of pentobarbital sodium (35 mg/kg). The expression levels of Clock mRNA and Bmal 1 mRNA in the hypothalamic tissues containing ventrolateral preoptic area (VLPO) and suprachiasmatic nucleus (SCN) region were detected by fluorescence quantitative real-time PCR. RESULTS: Following administration of pentobarbital sodium,the sleep latency was significantly prolonged and the sleep duration was considerably shortened in rats of the model group(P<0.05). After the treatment, the increased sleep latencies in the GV 20-HT 7, GV 20-SP 6 and GV 20-non-acupoints were all significantly down-regulated (P<0.05), and the decreased sleep duration was significantly increased only in the GV 20-HT 7 group relevant to the model group (P<0.05), but not in the GV 20-SP 6 and GV 20-non-acupoint groups (P<0.05). There were no significant differences in the sleep latency among the 3 treatment groups (P<0.05). The sleep duration was obviously prolonged in the GV 20-HT 7 group than in the GV 20-SP 6 and GV 20-non-acupoint groups (P<0.05). After modeling, the expression levels of Clock mRNA and Bmal 1 mRNA in hypothalamic VLPO and SCN regions were significantly down-regulated relevant to the normal control group (P<0.01). Following the treatment, the expression levels of Clock mRNA in the VLPO and SCN regions of the GV 20-SP 6 and GV 20-HT 7 groups, and those of Bmal 1 mRNA in the VLPO and SCN regions of the 3 treatment groups were considerably increased relevant to the model group (P<0.05, P<0.01). The effects of GV 20-HT 7 were significantly superior to those of GV 20-SP 6 and GV 20-non-acupoint (and also the action of GV 20-SP 6 was evidently superior to that of GV 20-non-acupoint) in up-regulating the expression of Clock mRNA and Bmal 1 mRNA in both VLPO and SCN regions (P<0.05, P<0.01). CONCLUSIONS: Manual acupuncture stimulation of GV 20-HT 7 can improve the sleep latency and duration in insomnia rats,which may be associated with its effects in up-regulating the expression levels of circadian Clock mRNA and Bmal 1 mRNA in hypothalamic VLPO and SCN regions, and the efficacy of GV 20-HT 7 is obviously better than that of GV 20-SP 6 and GV 20-non-acupoint. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29105472/[Effect_of_Acupuncture_Stimulation_of_Different_Acupoint_Combinations_on_Sleep_and_Expression_of_Circadian_Clock_and_Bmal_1_Genes_in_Hypothalamus_of_Insomnia_Rats]_ L2 - https://medlineplus.gov/insomnia.html DB - PRIME DP - Unbound Medicine ER -