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Maximal Exercise Alters the Inflammatory Phenotype and Response of Mononuclear Cells.
Med Sci Sports Exerc. 2018 04; 50(4):675-683.MS

Abstract

PURPOSE

Monocytes express the CD14 receptor that facilitates lipopolysaccharide (LPS) ligation to toll-like receptor 4 (TLR4) to elicit production of interleukin (IL)-6, IL-10, and tumor necrosis factor alpha (TNF-α). However, proinflammatory conditions, such as strenuous exercise, increase the percentage of monocytes expressing CD16, a receptor that enhances LPS stimulated TNF-α production. Therefore, we examined whether maximal treadmill exercise would alter the inflammatory phenotype of classical (CD14/CD16) and proinflammatory monocytes (intermediate [CD14/CD16] and nonclassical [CD14/CD16]), evidenced by changes in TLR4, CD14, and CD16 receptor expression, and their inflammatory response to ex vivo LPS stimulation.

METHODS

Human mononuclear cells from 25 male participants (age, 24.2 ± 4.0 yr) were isolated before and after exercise to assess TLR4, CD14, and CD16 expression by flow cytometry and ex vivo production of LPS-stimulated inflammatory cytokines (IL-6, IL-10, and TNF-α).

RESULTS

Exercise reduced the percentage of classical monocytes and increased the percentage of intermediate and nonclassical monocytes. In addition, TLR4 expression decreased on classical and intermediate monocytes, but not the nonclassical monocyte subset. Furthermore, although CD14 expression decreased on all monocyte subsets, CD16 expression increased on intermediate monocytes only. In parallel with these phenotypic changes, the inflammatory milieu shifted toward a proinflammatory response after LPS stimulation (decreased IL-6 and IL-10 and increased IL-6 to IL-10 ratio and TNF-α production).

CONCLUSIONS

These findings demonstrate that acute maximal exercise elicits a proinflammatory phenotype of isolated monocytes exposed to LPS and highlight potential mechanisms that will help elucidate the role of acute and chronic exercise on the innate immune response of circulating monocytes.

Authors+Show Affiliations

Department of Kinesiology and Health Sciences, Virginia Commonwealth University, Richmond, VA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29112629

Citation

Slusher, Aaron L., et al. "Maximal Exercise Alters the Inflammatory Phenotype and Response of Mononuclear Cells." Medicine and Science in Sports and Exercise, vol. 50, no. 4, 2018, pp. 675-683.
Slusher AL, Zúñiga TM, Acevedo EO. Maximal Exercise Alters the Inflammatory Phenotype and Response of Mononuclear Cells. Med Sci Sports Exerc. 2018;50(4):675-683.
Slusher, A. L., Zúñiga, T. M., & Acevedo, E. O. (2018). Maximal Exercise Alters the Inflammatory Phenotype and Response of Mononuclear Cells. Medicine and Science in Sports and Exercise, 50(4), 675-683. https://doi.org/10.1249/MSS.0000000000001480
Slusher AL, Zúñiga TM, Acevedo EO. Maximal Exercise Alters the Inflammatory Phenotype and Response of Mononuclear Cells. Med Sci Sports Exerc. 2018;50(4):675-683. PubMed PMID: 29112629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maximal Exercise Alters the Inflammatory Phenotype and Response of Mononuclear Cells. AU - Slusher,Aaron L, AU - Zúñiga,Tiffany M, AU - Acevedo,Edmund O, PY - 2017/11/8/pubmed PY - 2019/1/16/medline PY - 2017/11/8/entrez SP - 675 EP - 683 JF - Medicine and science in sports and exercise JO - Med Sci Sports Exerc VL - 50 IS - 4 N2 - PURPOSE: Monocytes express the CD14 receptor that facilitates lipopolysaccharide (LPS) ligation to toll-like receptor 4 (TLR4) to elicit production of interleukin (IL)-6, IL-10, and tumor necrosis factor alpha (TNF-α). However, proinflammatory conditions, such as strenuous exercise, increase the percentage of monocytes expressing CD16, a receptor that enhances LPS stimulated TNF-α production. Therefore, we examined whether maximal treadmill exercise would alter the inflammatory phenotype of classical (CD14/CD16) and proinflammatory monocytes (intermediate [CD14/CD16] and nonclassical [CD14/CD16]), evidenced by changes in TLR4, CD14, and CD16 receptor expression, and their inflammatory response to ex vivo LPS stimulation. METHODS: Human mononuclear cells from 25 male participants (age, 24.2 ± 4.0 yr) were isolated before and after exercise to assess TLR4, CD14, and CD16 expression by flow cytometry and ex vivo production of LPS-stimulated inflammatory cytokines (IL-6, IL-10, and TNF-α). RESULTS: Exercise reduced the percentage of classical monocytes and increased the percentage of intermediate and nonclassical monocytes. In addition, TLR4 expression decreased on classical and intermediate monocytes, but not the nonclassical monocyte subset. Furthermore, although CD14 expression decreased on all monocyte subsets, CD16 expression increased on intermediate monocytes only. In parallel with these phenotypic changes, the inflammatory milieu shifted toward a proinflammatory response after LPS stimulation (decreased IL-6 and IL-10 and increased IL-6 to IL-10 ratio and TNF-α production). CONCLUSIONS: These findings demonstrate that acute maximal exercise elicits a proinflammatory phenotype of isolated monocytes exposed to LPS and highlight potential mechanisms that will help elucidate the role of acute and chronic exercise on the innate immune response of circulating monocytes. SN - 1530-0315 UR - https://www.unboundmedicine.com/medline/citation/29112629/Maximal_Exercise_Alters_the_Inflammatory_Phenotype_and_Response_of_Mononuclear_Cells_ L2 - http://dx.doi.org/10.1249/MSS.0000000000001480 DB - PRIME DP - Unbound Medicine ER -