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Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice.

Abstract

OBJECTIVES

Increases in astrocytes and one of their markers, glial fibrillary acidic protein (GFAP) have been reported in the brains of patients with Alzheimer's disease (AD). N-3 polyunsaturated fatty acids (PUFA) modulate neuroinflammation in animal models; however, their effect on astrocytes is unclear.

METHODS

Fat-1 mice and their wildtype littermates were fed either a fish oil diet or a safflower oil diet deprived of n-3 PUFA. At 12 weeks, mice underwent intracerebroventricular infusion of amyloid-β 1-40. Astrocyte phenotype in the hippocampus was assessed at baseline and 10 days post-surgery using immunohistochemistry with various microscopy and image analysis techniques.

RESULTS

GFAP increased in all groups in response to amyloid-β, with a greater increase in fish oil-fed mice than either fat-1 or wildtype safflower oil-fed mice. Astrocytes in this group were also more hypertrophic, suggesting increased activation. Both fat-1- and fish oil-fed mice had greater increases in branch number and length in response to amyloid-β infusion than wildtype safflower animals.

CONCLUSION

Fish oil feeding, and to a lesser extent the fat-1 transgene, enhances the astrocyte activation phenotype in response to amyloid-β 1-40. Astrocytes in mice fed fish oil were more activated in response to amyloid-β than in fat-1 mice despite similar levels of hippocampal n-3 PUFA, which suggests that other fatty acids or dietary factors contribute to this effect.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.

    ,

    a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.

    ,

    b Department of Pharmacology and Toxicology , University of Toronto , Toronto , Canada.

    ,

    a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.

    a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.

    Source

    Nutritional neuroscience 22:6 2019 Jun pg 418-424

    MeSH

    Amyloid beta-Peptides
    Animals
    Astrocytes
    Caenorhabditis elegans Proteins
    Encephalitis
    Fatty Acid Desaturases
    Fatty Acids, Omega-3
    Glial Fibrillary Acidic Protein
    Hippocampus
    Infusions, Intraventricular
    Male
    Mice, Inbred C57BL
    Mice, Transgenic
    Peptide Fragments
    Safflower Oil
    Transgenes

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29113539

    Citation

    Hopperton, Kathryn E., et al. "Dietary Fish Oil, and to a Lesser Extent the Fat-1 Transgene, Increases Astrocyte Activation in Response to Intracerebroventricular Amyloid-β 1-40 in Mice." Nutritional Neuroscience, vol. 22, no. 6, 2019, pp. 418-424.
    Hopperton KE, James NCE, Mohammad D, et al. Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice. Nutr Neurosci. 2019;22(6):418-424.
    Hopperton, K. E., James, N. C. E., Mohammad, D., Irfan, M., & Bazinet, R. P. (2019). Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice. Nutritional Neuroscience, 22(6), pp. 418-424. doi:10.1080/1028415X.2017.1396068.
    Hopperton KE, et al. Dietary Fish Oil, and to a Lesser Extent the Fat-1 Transgene, Increases Astrocyte Activation in Response to Intracerebroventricular Amyloid-β 1-40 in Mice. Nutr Neurosci. 2019;22(6):418-424. PubMed PMID: 29113539.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice. AU - Hopperton,Kathryn E, AU - James,Nicholas C E, AU - Mohammad,Dana, AU - Irfan,Maha, AU - Bazinet,Richard P, Y1 - 2017/11/07/ PY - 2017/11/9/pubmed PY - 2019/5/24/medline PY - 2017/11/9/entrez KW - Alzheimer’s disease KW - Amyloid-β KW - Astrocyte KW - Docosahexaenoic acid KW - Fish KW - Neuroinflammation KW - n-3 Polyunsaturated fatty acids SP - 418 EP - 424 JF - Nutritional neuroscience JO - Nutr Neurosci VL - 22 IS - 6 N2 - OBJECTIVES: Increases in astrocytes and one of their markers, glial fibrillary acidic protein (GFAP) have been reported in the brains of patients with Alzheimer's disease (AD). N-3 polyunsaturated fatty acids (PUFA) modulate neuroinflammation in animal models; however, their effect on astrocytes is unclear. METHODS: Fat-1 mice and their wildtype littermates were fed either a fish oil diet or a safflower oil diet deprived of n-3 PUFA. At 12 weeks, mice underwent intracerebroventricular infusion of amyloid-β 1-40. Astrocyte phenotype in the hippocampus was assessed at baseline and 10 days post-surgery using immunohistochemistry with various microscopy and image analysis techniques. RESULTS: GFAP increased in all groups in response to amyloid-β, with a greater increase in fish oil-fed mice than either fat-1 or wildtype safflower oil-fed mice. Astrocytes in this group were also more hypertrophic, suggesting increased activation. Both fat-1- and fish oil-fed mice had greater increases in branch number and length in response to amyloid-β infusion than wildtype safflower animals. CONCLUSION: Fish oil feeding, and to a lesser extent the fat-1 transgene, enhances the astrocyte activation phenotype in response to amyloid-β 1-40. Astrocytes in mice fed fish oil were more activated in response to amyloid-β than in fat-1 mice despite similar levels of hippocampal n-3 PUFA, which suggests that other fatty acids or dietary factors contribute to this effect. SN - 1476-8305 UR - https://www.unboundmedicine.com/medline/citation/29113539/Dietary_fish_oil_and_to_a_lesser_extent_the_fat_1_transgene_increases_astrocyte_activation_in_response_to_intracerebroventricular_amyloid_β_1_40_in_mice_ L2 - http://www.tandfonline.com/doi/full/10.1080/1028415X.2017.1396068 DB - PRIME DP - Unbound Medicine ER -