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Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice.
Nutr Neurosci 2019; 22(6):418-424NN

Abstract

OBJECTIVES

Increases in astrocytes and one of their markers, glial fibrillary acidic protein (GFAP) have been reported in the brains of patients with Alzheimer's disease (AD). N-3 polyunsaturated fatty acids (PUFA) modulate neuroinflammation in animal models; however, their effect on astrocytes is unclear.

METHODS

Fat-1 mice and their wildtype littermates were fed either a fish oil diet or a safflower oil diet deprived of n-3 PUFA. At 12 weeks, mice underwent intracerebroventricular infusion of amyloid-β 1-40. Astrocyte phenotype in the hippocampus was assessed at baseline and 10 days post-surgery using immunohistochemistry with various microscopy and image analysis techniques.

RESULTS

GFAP increased in all groups in response to amyloid-β, with a greater increase in fish oil-fed mice than either fat-1 or wildtype safflower oil-fed mice. Astrocytes in this group were also more hypertrophic, suggesting increased activation. Both fat-1- and fish oil-fed mice had greater increases in branch number and length in response to amyloid-β infusion than wildtype safflower animals.

CONCLUSION

Fish oil feeding, and to a lesser extent the fat-1 transgene, enhances the astrocyte activation phenotype in response to amyloid-β 1-40. Astrocytes in mice fed fish oil were more activated in response to amyloid-β than in fat-1 mice despite similar levels of hippocampal n-3 PUFA, which suggests that other fatty acids or dietary factors contribute to this effect.

Authors+Show Affiliations

a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.b Department of Pharmacology and Toxicology , University of Toronto , Toronto , Canada.a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.a Department of Nutritional Sciences , University of Toronto , Toronto , Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29113539

Citation

Hopperton, Kathryn E., et al. "Dietary Fish Oil, and to a Lesser Extent the Fat-1 Transgene, Increases Astrocyte Activation in Response to Intracerebroventricular Amyloid-β 1-40 in Mice." Nutritional Neuroscience, vol. 22, no. 6, 2019, pp. 418-424.
Hopperton KE, James NCE, Mohammad D, et al. Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice. Nutr Neurosci. 2019;22(6):418-424.
Hopperton, K. E., James, N. C. E., Mohammad, D., Irfan, M., & Bazinet, R. P. (2019). Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice. Nutritional Neuroscience, 22(6), pp. 418-424. doi:10.1080/1028415X.2017.1396068.
Hopperton KE, et al. Dietary Fish Oil, and to a Lesser Extent the Fat-1 Transgene, Increases Astrocyte Activation in Response to Intracerebroventricular Amyloid-β 1-40 in Mice. Nutr Neurosci. 2019;22(6):418-424. PubMed PMID: 29113539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary fish oil, and to a lesser extent the fat-1 transgene, increases astrocyte activation in response to intracerebroventricular amyloid-β 1-40 in mice. AU - Hopperton,Kathryn E, AU - James,Nicholas C E, AU - Mohammad,Dana, AU - Irfan,Maha, AU - Bazinet,Richard P, Y1 - 2017/11/07/ PY - 2017/11/9/pubmed PY - 2019/5/24/medline PY - 2017/11/9/entrez KW - Alzheimer’s disease KW - Amyloid-β KW - Astrocyte KW - Docosahexaenoic acid KW - Fish KW - Neuroinflammation KW - n-3 Polyunsaturated fatty acids SP - 418 EP - 424 JF - Nutritional neuroscience JO - Nutr Neurosci VL - 22 IS - 6 N2 - OBJECTIVES: Increases in astrocytes and one of their markers, glial fibrillary acidic protein (GFAP) have been reported in the brains of patients with Alzheimer's disease (AD). N-3 polyunsaturated fatty acids (PUFA) modulate neuroinflammation in animal models; however, their effect on astrocytes is unclear. METHODS: Fat-1 mice and their wildtype littermates were fed either a fish oil diet or a safflower oil diet deprived of n-3 PUFA. At 12 weeks, mice underwent intracerebroventricular infusion of amyloid-β 1-40. Astrocyte phenotype in the hippocampus was assessed at baseline and 10 days post-surgery using immunohistochemistry with various microscopy and image analysis techniques. RESULTS: GFAP increased in all groups in response to amyloid-β, with a greater increase in fish oil-fed mice than either fat-1 or wildtype safflower oil-fed mice. Astrocytes in this group were also more hypertrophic, suggesting increased activation. Both fat-1- and fish oil-fed mice had greater increases in branch number and length in response to amyloid-β infusion than wildtype safflower animals. CONCLUSION: Fish oil feeding, and to a lesser extent the fat-1 transgene, enhances the astrocyte activation phenotype in response to amyloid-β 1-40. Astrocytes in mice fed fish oil were more activated in response to amyloid-β than in fat-1 mice despite similar levels of hippocampal n-3 PUFA, which suggests that other fatty acids or dietary factors contribute to this effect. SN - 1476-8305 UR - https://www.unboundmedicine.com/medline/citation/29113539/Dietary_fish_oil_and_to_a_lesser_extent_the_fat_1_transgene_increases_astrocyte_activation_in_response_to_intracerebroventricular_amyloid_β_1_40_in_mice_ L2 - http://www.tandfonline.com/doi/full/10.1080/1028415X.2017.1396068 DB - PRIME DP - Unbound Medicine ER -