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Spectroscopic determination of succinylcholine in dosage forms using eosin Y.
Luminescence. 2018 Mar; 33(2):376-381.L

Abstract

Two simple and sensitive analytical assay methods using spectrophotometry and spectrofluorimetry techniques were developed for the estimation of succinylcholine chloride (SUC) in pharmaceutical preparations. The suggested methods are based on the formation of an ion pair complex formed between the drug and eosin Y spectrophotometrically (Method I), or the suppressive effect of succinylcholine on the native fluorescence property of eosin Y (Method II). The spectrophotometric method (Method I) involves measuring the absorbance of the complex between succinylcholine and eosin Y at 550 nm in Britton Robinson buffer of pH 3. However, the spectrofluorimetric method (Method II) involves measuring the quenching effect of the studied drug on the native fluorescence property of eosin Y at the same pH at 550 nm after excitation at 480 nm. The absorbance versus concentration of the drug is rectilinear over the range of 0.5 to 15 μg/ml. The formation constant was 3.5 × 104 and the Gibb's free energy change was -2.5 × 104 J/mol. In Method II, the relative fluorescence intensity was directly proportional to SUC concentration over the range of 0.05 to 1 μg/ml. The proposed methods allowed a successful application to the estimation of succinylcholine ampoules. An explanation of the reaction pathway was postulated.

Authors+Show Affiliations

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

29115039

Citation

Ayad, Magda M., et al. "Spectroscopic Determination of Succinylcholine in Dosage Forms Using Eosin Y." Luminescence : the Journal of Biological and Chemical Luminescence, vol. 33, no. 2, 2018, pp. 376-381.
Ayad MM, Belal F, Hosney MM, et al. Spectroscopic determination of succinylcholine in dosage forms using eosin Y. Luminescence. 2018;33(2):376-381.
Ayad, M. M., Belal, F., Hosney, M. M., Abo El Abass, S., & Elsayed, N. (2018). Spectroscopic determination of succinylcholine in dosage forms using eosin Y. Luminescence : the Journal of Biological and Chemical Luminescence, 33(2), 376-381. https://doi.org/10.1002/bio.3424
Ayad MM, et al. Spectroscopic Determination of Succinylcholine in Dosage Forms Using Eosin Y. Luminescence. 2018;33(2):376-381. PubMed PMID: 29115039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spectroscopic determination of succinylcholine in dosage forms using eosin Y. AU - Ayad,Magda M, AU - Belal,Fathalla, AU - Hosney,Mervet M, AU - Abo El Abass,Samah, AU - Elsayed,Nora, Y1 - 2017/11/08/ PY - 2017/07/22/received PY - 2017/09/13/revised PY - 2017/09/29/accepted PY - 2017/11/9/pubmed PY - 2018/9/5/medline PY - 2017/11/9/entrez KW - eosin Y KW - spectrofluorimetry KW - spectrophotometry KW - succinylcholine SP - 376 EP - 381 JF - Luminescence : the journal of biological and chemical luminescence JO - Luminescence VL - 33 IS - 2 N2 - Two simple and sensitive analytical assay methods using spectrophotometry and spectrofluorimetry techniques were developed for the estimation of succinylcholine chloride (SUC) in pharmaceutical preparations. The suggested methods are based on the formation of an ion pair complex formed between the drug and eosin Y spectrophotometrically (Method I), or the suppressive effect of succinylcholine on the native fluorescence property of eosin Y (Method II). The spectrophotometric method (Method I) involves measuring the absorbance of the complex between succinylcholine and eosin Y at 550 nm in Britton Robinson buffer of pH 3. However, the spectrofluorimetric method (Method II) involves measuring the quenching effect of the studied drug on the native fluorescence property of eosin Y at the same pH at 550 nm after excitation at 480 nm. The absorbance versus concentration of the drug is rectilinear over the range of 0.5 to 15 μg/ml. The formation constant was 3.5 × 104 and the Gibb's free energy change was -2.5 × 104 J/mol. In Method II, the relative fluorescence intensity was directly proportional to SUC concentration over the range of 0.05 to 1 μg/ml. The proposed methods allowed a successful application to the estimation of succinylcholine ampoules. An explanation of the reaction pathway was postulated. SN - 1522-7243 UR - https://www.unboundmedicine.com/medline/citation/29115039/Spectroscopic_determination_of_succinylcholine_in_dosage_forms_using_eosin_Y_ L2 - https://doi.org/10.1002/bio.3424 DB - PRIME DP - Unbound Medicine ER -