TY - JOUR T1 - Validated thin-layer chromatographic method for the identification and monitoring of the effect of the extraction method on the yield and phytochemical constituents of Egyptian Withania somnifera leaves. AU - Mahrous,Rahma S R, AU - Fathy,Hoda M, AU - Abu El-Khair,Rasha M, AU - Omar,Abdallah A, Y1 - 2017/11/30/ PY - 2017/07/11/received PY - 2017/09/19/revised PY - 2017/10/27/accepted PY - 2017/11/9/pubmed PY - 2018/10/23/medline PY - 2017/11/9/entrez KW - Withania somnifera KW - chemotype III KW - ultrasound-assisted extraction KW - withanolide S SP - 518 EP - 524 JF - Journal of separation science JO - J Sep Sci VL - 41 IS - 2 N2 - A sensitive, reliable, simple and rapid thin-layer chromatographic method has been developed for routine analysis of withanolide S content for the purpose of quality control assessment of chemotype III of Withania somnifera. The new method was used first to compare the accumulation of withanolide S in different parts of the plant, which was found to be the highest in the leaves extract (0.21% w/w). Second, to investigate different extraction parameters that improve the extraction efficiency of withanolides from the leaves using conventional and ultrasound-assisted extraction methods. The extraction efficiency was expressed via total withanolide content and withanolide S content. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/29115713/Validated_thin_layer_chromatographic_method_for_the_identification_and_monitoring_of_the_effect_of_the_extraction_method_on_the_yield_and_phytochemical_constituents_of_Egyptian_Withania_somnifera_leaves_ DB - PRIME DP - Unbound Medicine ER -