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Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2).
Parasite. 2017; 24:41.P

Abstract

Toxoplasma gondii, an intracellular zoonotic protozoan parasite, is possibly the most widespread parasite of warm-blooded animals and can cause serious public health problems and economic losses worldwide. TgCDPK2, a member of the T. gondii calcium-dependent protein kinase family, was recently identified as an essential regulator for viable cyst development in T. gondii. In the present study, we evaluated the protective immunity induced by DNA vaccination based on a recombinant eukaryotic plasmid, pVAX-TgCDPK2, against acute toxoplasmosis in mice. BALB/c mice were intramuscularly immunized with pVAX-TgCDPK2 plasmid and then challenged by infection with the highly virulent RH strain of T. gondii. The specific immune responses and protective efficacy against T. gondii were analyzed by cytokine and serum antibody measurements, lymphocyte proliferation assays, flow cytometric on lymphocytes and the survival time of mice after challenge. Our results showed that mice immunized with pVAX-TgCDPK2 could elicit special humoral and cellular responses, with higher levels of IgG antibody, and increased levels of Th1-type cytokines IFN-γ, IL-12(p70), and CD3 + CD4 + CD8 - and CD3 + CD8 + CD4 - T cells, and had a prolonged survival time (14.0 ± 2.32 days) compared to control mice. These results demonstrate that pVAX-TgCDPK2 is a potential vaccine candidate against acute toxoplasmosis.

Authors+Show Affiliations

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China - College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin, Heilongjiang Province 150030, China.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China - Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29119944

Citation

Chen, Kai, et al. "Immune Responses and Protection After DNA Vaccination Against Toxoplasma Gondii Calcium-dependent Protein Kinase 2 (TgCDPK2)." Parasite (Paris, France), vol. 24, 2017, p. 41.
Chen K, Wang JL, Huang SY, et al. Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2). Parasite. 2017;24:41.
Chen, K., Wang, J. L., Huang, S. Y., Yang, W. B., Zhu, W. N., & Zhu, X. Q. (2017). Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2). Parasite (Paris, France), 24, 41. https://doi.org/10.1051/parasite/2017045
Chen K, et al. Immune Responses and Protection After DNA Vaccination Against Toxoplasma Gondii Calcium-dependent Protein Kinase 2 (TgCDPK2). Parasite. 2017;24:41. PubMed PMID: 29119944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2). AU - Chen,Kai, AU - Wang,Jin-Lei, AU - Huang,Si-Yang, AU - Yang,Wen-Bin, AU - Zhu,Wei-Ning, AU - Zhu,Xing-Quan, Y1 - 2017/11/09/ PY - 2017/05/10/received PY - 2017/09/04/accepted PY - 2017/11/10/entrez PY - 2017/11/10/pubmed PY - 2018/7/11/medline SP - 41 EP - 41 JF - Parasite (Paris, France) JO - Parasite VL - 24 N2 - Toxoplasma gondii, an intracellular zoonotic protozoan parasite, is possibly the most widespread parasite of warm-blooded animals and can cause serious public health problems and economic losses worldwide. TgCDPK2, a member of the T. gondii calcium-dependent protein kinase family, was recently identified as an essential regulator for viable cyst development in T. gondii. In the present study, we evaluated the protective immunity induced by DNA vaccination based on a recombinant eukaryotic plasmid, pVAX-TgCDPK2, against acute toxoplasmosis in mice. BALB/c mice were intramuscularly immunized with pVAX-TgCDPK2 plasmid and then challenged by infection with the highly virulent RH strain of T. gondii. The specific immune responses and protective efficacy against T. gondii were analyzed by cytokine and serum antibody measurements, lymphocyte proliferation assays, flow cytometric on lymphocytes and the survival time of mice after challenge. Our results showed that mice immunized with pVAX-TgCDPK2 could elicit special humoral and cellular responses, with higher levels of IgG antibody, and increased levels of Th1-type cytokines IFN-γ, IL-12(p70), and CD3 + CD4 + CD8 - and CD3 + CD8 + CD4 - T cells, and had a prolonged survival time (14.0 ± 2.32 days) compared to control mice. These results demonstrate that pVAX-TgCDPK2 is a potential vaccine candidate against acute toxoplasmosis. SN - 1776-1042 UR - https://www.unboundmedicine.com/medline/citation/29119944/Immune_responses_and_protection_after_DNA_vaccination_against_Toxoplasma_gondii_calcium_dependent_protein_kinase_2__TgCDPK2__ L2 - http://publications.edpsciences.org/10.1051/parasite/2017045 DB - PRIME DP - Unbound Medicine ER -