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Antibacterial activities of the phytochemicals-characterized extracts of Callistemon viminalis, Eucalyptus camaldulensis and Conyza dioscoridis against the growth of some phytopathogenic bacteria.
Microb Pathog 2017; 113:348-356MP

Abstract

Three bacterial isolates were isolated from infected potato tubers showing soft and brown rots like symptoms as well as one isolate from infected peach tree showing crown gall symptom. The morphological, biochemical and molecular assays proved that bacterial isolates belonging to Pectobacterium carotovorum subsp. carotovorum, Ralstonia solanacearum, Dickeya spp. and Agrobacterium tumefaciens. The acetone (AcE) and n-butanol (ButE) extracts of Callistemon viminalis flowers and essential oil from aerial parts of Conyza dioscoridis as well as ButE of Eucalyptus camaldulensis bark are evaluated at different concentrations against the growth of the isolated bacteria. The diameter of inhibition zone (IZ) and the minimum inhibitory concentrations (MICs) are compared. Results indicated that the highest IZ values were 20.0 mm and 18.3 mm for E. camaldulensis bark ButE and C. viminalis flower ButE, respectively, against P. carotovorum; 16.3 mm and 16.0 mm for E. camaldulensis bark ButE and C. viminalis flower ButE, respectively, against R. solanacearum; 18.5 mm for C. viminalis flower AcE and C. dioscoridis aerial parts EO against Dickeya spp.; and 15.0 mm for C. viminalis flower AcE against A. tumefaciens. MICs ranged from <16 μg/mL for D. solani to >4000 μg/mL for A. tumefaciens. It was proved that C. viminalis flowers AcE contains mainly 5-hydroxymethylfurfural (20.6%), palmitic acid (18.5%), and pyrogallol (16.4%); while C. viminalis flower ButE contains palmitic acid (36.3%), 2-hydroxymyristic acid (9.4%), 5-hydroxymethylfurfural (7.2%), and shikimic acid (6.6%); whereas E. camaldulensis bark ButE contains 8-nonynoic acid methyl ester (45.6), camphor (30.9%), menthol (8.8%), and 1,8-cineole (eucalyptol) (8.2%), whilst the EO of C. dioscoridis aerial parts comprises Z-(13,14-epoxy)tetradec-11-en-1-ol acetate (11.6%), γ-elemene (10.2%), tau.-muurolol (7.1%), and cadina-3,9-diene (4.7%). It can be concluded that phytochemical extracts of C. viminalis, E. camaldulensis and C. dioscoridis demonstrated strong to moderate antibacterial effects against the studied plant bacterial pathogens.

Authors+Show Affiliations

Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt.Department of Plant Pathology, Faculty of Agriculture (ELShatby), Alexandria University, Alexandria, Egypt.Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, Egypt.Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Estado de México, Mexico. Electronic address: asalem70@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29126952

Citation

El-Hefny, Mervat, et al. "Antibacterial Activities of the Phytochemicals-characterized Extracts of Callistemon Viminalis, Eucalyptus Camaldulensis and Conyza Dioscoridis Against the Growth of some Phytopathogenic Bacteria." Microbial Pathogenesis, vol. 113, 2017, pp. 348-356.
El-Hefny M, Ashmawy NA, Salem MZM, et al. Antibacterial activities of the phytochemicals-characterized extracts of Callistemon viminalis, Eucalyptus camaldulensis and Conyza dioscoridis against the growth of some phytopathogenic bacteria. Microb Pathog. 2017;113:348-356.
El-Hefny, M., Ashmawy, N. A., Salem, M. Z. M., & Salem, A. Z. M. (2017). Antibacterial activities of the phytochemicals-characterized extracts of Callistemon viminalis, Eucalyptus camaldulensis and Conyza dioscoridis against the growth of some phytopathogenic bacteria. Microbial Pathogenesis, 113, pp. 348-356. doi:10.1016/j.micpath.2017.11.004.
El-Hefny M, et al. Antibacterial Activities of the Phytochemicals-characterized Extracts of Callistemon Viminalis, Eucalyptus Camaldulensis and Conyza Dioscoridis Against the Growth of some Phytopathogenic Bacteria. Microb Pathog. 2017;113:348-356. PubMed PMID: 29126952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial activities of the phytochemicals-characterized extracts of Callistemon viminalis, Eucalyptus camaldulensis and Conyza dioscoridis against the growth of some phytopathogenic bacteria. AU - El-Hefny,Mervat, AU - Ashmawy,Nader A, AU - Salem,Mohamed Z M, AU - Salem,Abdelfattah Z M, Y1 - 2017/11/07/ PY - 2017/10/15/received PY - 2017/10/31/revised PY - 2017/11/02/accepted PY - 2017/11/12/pubmed PY - 2018/7/22/medline PY - 2017/11/12/entrez KW - Callistemon viminalis KW - Conyza dioscoridis KW - Eucalyptus camaldulensis KW - Phytochemicals KW - Phytopathogenic bacteria SP - 348 EP - 356 JF - Microbial pathogenesis JO - Microb. Pathog. VL - 113 N2 - Three bacterial isolates were isolated from infected potato tubers showing soft and brown rots like symptoms as well as one isolate from infected peach tree showing crown gall symptom. The morphological, biochemical and molecular assays proved that bacterial isolates belonging to Pectobacterium carotovorum subsp. carotovorum, Ralstonia solanacearum, Dickeya spp. and Agrobacterium tumefaciens. The acetone (AcE) and n-butanol (ButE) extracts of Callistemon viminalis flowers and essential oil from aerial parts of Conyza dioscoridis as well as ButE of Eucalyptus camaldulensis bark are evaluated at different concentrations against the growth of the isolated bacteria. The diameter of inhibition zone (IZ) and the minimum inhibitory concentrations (MICs) are compared. Results indicated that the highest IZ values were 20.0 mm and 18.3 mm for E. camaldulensis bark ButE and C. viminalis flower ButE, respectively, against P. carotovorum; 16.3 mm and 16.0 mm for E. camaldulensis bark ButE and C. viminalis flower ButE, respectively, against R. solanacearum; 18.5 mm for C. viminalis flower AcE and C. dioscoridis aerial parts EO against Dickeya spp.; and 15.0 mm for C. viminalis flower AcE against A. tumefaciens. MICs ranged from <16 μg/mL for D. solani to >4000 μg/mL for A. tumefaciens. It was proved that C. viminalis flowers AcE contains mainly 5-hydroxymethylfurfural (20.6%), palmitic acid (18.5%), and pyrogallol (16.4%); while C. viminalis flower ButE contains palmitic acid (36.3%), 2-hydroxymyristic acid (9.4%), 5-hydroxymethylfurfural (7.2%), and shikimic acid (6.6%); whereas E. camaldulensis bark ButE contains 8-nonynoic acid methyl ester (45.6), camphor (30.9%), menthol (8.8%), and 1,8-cineole (eucalyptol) (8.2%), whilst the EO of C. dioscoridis aerial parts comprises Z-(13,14-epoxy)tetradec-11-en-1-ol acetate (11.6%), γ-elemene (10.2%), tau.-muurolol (7.1%), and cadina-3,9-diene (4.7%). It can be concluded that phytochemical extracts of C. viminalis, E. camaldulensis and C. dioscoridis demonstrated strong to moderate antibacterial effects against the studied plant bacterial pathogens. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/29126952/Antibacterial_activities_of_the_phytochemicals_characterized_extracts_of_Callistemon_viminalis_Eucalyptus_camaldulensis_and_Conyza_dioscoridis_against_the_growth_of_some_phytopathogenic_bacteria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(17)31322-0 DB - PRIME DP - Unbound Medicine ER -