Tags

Type your tag names separated by a space and hit enter

Egg Case Silk Gene Sequences from Argiope Spiders: Evidence for Multiple Loci and a Loss of Function Between Paralogs.
G3 (Bethesda). 2018 01 04; 8(1):231-238.G

Abstract

Spiders swath their eggs with silk to protect developing embryos and hatchlings. Egg case silks, like other fibrous spider silks, are primarily composed of proteins called spidroins (spidroin = spider-fibroin). Silks, and thus spidroins, are important throughout the lives of spiders, yet the evolution of spidroin genes has been relatively understudied. Spidroin genes are notoriously difficult to sequence because they are typically very long (≥ 10 kb of coding sequence) and highly repetitive. Here, we investigate the evolution of spider silk genes through long-read sequencing of Bacterial Artificial Chromosome (BAC) clones. We demonstrate that the silver garden spider Argiope argentata has multiple egg case spidroin loci with a loss of function at one locus. We also use degenerate PCR primers to search the genomic DNA of congeneric species and find evidence for multiple egg case spidroin loci in other Argiope spiders. Comparative analyses show that these multiple loci are more similar at the nucleotide level within a species than between species. This pattern is consistent with concerted evolution homogenizing gene copies within a genome. More complicated explanations include convergent evolution or recent independent gene duplications within each species.

Authors+Show Affiliations

Department of Biology, University of California, Riverside, California 92521 chaw@ohsu.edu.Department of Biology, University of California, Riverside, California 92521.Department of Biology, University of California, Riverside, California 92521.Department of Biology, University of California, Riverside, California 92521.Department of Biology, University of California, Riverside, California 92521. Division of Invertebrate Zoology, American Museum of Natural History, New York, New York 10024. Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, New York 10024.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

29127108

Citation

Chaw, R Crystal, et al. "Egg Case Silk Gene Sequences From Argiope Spiders: Evidence for Multiple Loci and a Loss of Function Between Paralogs." G3 (Bethesda, Md.), vol. 8, no. 1, 2018, pp. 231-238.
Chaw RC, Collin M, Wimmer M, et al. Egg Case Silk Gene Sequences from Argiope Spiders: Evidence for Multiple Loci and a Loss of Function Between Paralogs. G3 (Bethesda). 2018;8(1):231-238.
Chaw, R. C., Collin, M., Wimmer, M., Helmrick, K. L., & Hayashi, C. Y. (2018). Egg Case Silk Gene Sequences from Argiope Spiders: Evidence for Multiple Loci and a Loss of Function Between Paralogs. G3 (Bethesda, Md.), 8(1), 231-238. https://doi.org/10.1534/g3.117.300283
Chaw RC, et al. Egg Case Silk Gene Sequences From Argiope Spiders: Evidence for Multiple Loci and a Loss of Function Between Paralogs. G3 (Bethesda). 2018 01 4;8(1):231-238. PubMed PMID: 29127108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Egg Case Silk Gene Sequences from Argiope Spiders: Evidence for Multiple Loci and a Loss of Function Between Paralogs. AU - Chaw,R Crystal, AU - Collin,Matthew, AU - Wimmer,Marjorie, AU - Helmrick,Kara-Leigh, AU - Hayashi,Cheryl Y, Y1 - 2018/01/04/ PY - 2017/11/12/pubmed PY - 2018/10/10/medline PY - 2017/11/12/entrez KW - TuSp1 KW - evolution KW - gene family KW - spider silk KW - tubuliform spidroin SP - 231 EP - 238 JF - G3 (Bethesda, Md.) JO - G3 (Bethesda) VL - 8 IS - 1 N2 - Spiders swath their eggs with silk to protect developing embryos and hatchlings. Egg case silks, like other fibrous spider silks, are primarily composed of proteins called spidroins (spidroin = spider-fibroin). Silks, and thus spidroins, are important throughout the lives of spiders, yet the evolution of spidroin genes has been relatively understudied. Spidroin genes are notoriously difficult to sequence because they are typically very long (≥ 10 kb of coding sequence) and highly repetitive. Here, we investigate the evolution of spider silk genes through long-read sequencing of Bacterial Artificial Chromosome (BAC) clones. We demonstrate that the silver garden spider Argiope argentata has multiple egg case spidroin loci with a loss of function at one locus. We also use degenerate PCR primers to search the genomic DNA of congeneric species and find evidence for multiple egg case spidroin loci in other Argiope spiders. Comparative analyses show that these multiple loci are more similar at the nucleotide level within a species than between species. This pattern is consistent with concerted evolution homogenizing gene copies within a genome. More complicated explanations include convergent evolution or recent independent gene duplications within each species. SN - 2160-1836 UR - https://www.unboundmedicine.com/medline/citation/29127108/Egg_Case_Silk_Gene_Sequences_from_Argiope_Spiders:_Evidence_for_Multiple_Loci_and_a_Loss_of_Function_Between_Paralogs_ L2 - http://www.g3journal.org/cgi/pmidlookup?view=long&pmid=29127108 DB - PRIME DP - Unbound Medicine ER -