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Co-supplementation of isomalto-oligosaccharides potentiates metabolic health benefits of polyphenol-rich cranberry extract in high fat diet-fed mice via enhanced gut butyrate production.
Eur J Nutr 2018; 57(8):2897-2911EJ

Abstract

PURPOSE

Cranberries are a rich source of polyphenolic antioxidants. Purified sugars or artificial sweeteners are being added to cranberry-based food products to mask tartness. Refined sugar and artificial sweeteners intake modulate gut microbiota and result in metabolic complications. We evaluated effects of isomalto-oligosaccharides (IMOs; sweet tasting non-digestible oligosaccharides) with cranberry extract (CRX) on high fat diet (HFD)-induced metabolic alterations in mice.

METHODS

Male Swiss albino mice were fed normal chow or HFD (58% fat kcal), and were administered either CRX (200 mg/kg) alone or in combination with IMOs (1 g/kg). Cecal short-chain fatty acids, abundances of selected (1) butyrate producing, (2) metabolically beneficial, and (3) selective lipopolysaccharides producing gram negative gut bacteria were studied. Further, gut-related histological, biochemical, genomic changes along with circulating pro-/anti-inflammatory markers and systemic obesity-associated metabolic changes were studied.

RESULTS

Co-supplementation of CRX and IMOs significantly improved cecal SCFAs, especially butyrate levels, selected butyrate-producing bacteria (clostridial cluster XIVa bacteria) and butyrate kinase expression in HFD-fed mice. The combination also significantly improved gut beneficial bacterial abundance, gut histology and related changes (colon mucin production, gut permeability) as compared to individual agents. It also prevented HFD-induced systemic and tissue inflammation, glucose intolerance and systemic obesity-associated metabolic changes in adipose tissue and liver. The combination of CRX and IMOs appeared more effective in the prevention of HFD-induced gut derangements.

CONCLUSION

Combination of CRX and IMOs could be advantageous for normalization of metabolic alterations seen in diet-induced obesity via beneficial modulation of gastrointestinal health.

Authors+Show Affiliations

Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India. Pharmacology Division, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh, 160014, India. Lundberg Laboratory for Diabetes Research, Department of Molecular and Clinical Medicine, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SE-41345, Sweden.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India. Department of Pharmaceutical and Pharmacological Science, Katholieke Universiteit Leuven, Leuven, Belgium.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India.Department of Biotechnology, Government College for Girls, Sector 42, Chandigarh, India.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India. kiran@nabi.res.in.Pharmacology Division, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh, 160014, India. kanwaljitchopra@yahoo.co.in.Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Sector-81, Knowledge City, S.A.S. Nagar, P.O.- Manauli, Mohali, Punjab, 140306, India. mbishnoi@gmail.com. Functional Foods Research Laboratory, University of Southern Queensland, Toowoomba, 4350, Queensland, Australia. mbishnoi@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29127476

Citation

Singh, Dhirendra Pratap, et al. "Co-supplementation of Isomalto-oligosaccharides Potentiates Metabolic Health Benefits of Polyphenol-rich Cranberry Extract in High Fat Diet-fed Mice Via Enhanced Gut Butyrate Production." European Journal of Nutrition, vol. 57, no. 8, 2018, pp. 2897-2911.
Singh DP, Singh S, Bijalwan V, et al. Co-supplementation of isomalto-oligosaccharides potentiates metabolic health benefits of polyphenol-rich cranberry extract in high fat diet-fed mice via enhanced gut butyrate production. Eur J Nutr. 2018;57(8):2897-2911.
Singh, D. P., Singh, S., Bijalwan, V., Kumar, V., Khare, P., Baboota, R. K., ... Bishnoi, M. (2018). Co-supplementation of isomalto-oligosaccharides potentiates metabolic health benefits of polyphenol-rich cranberry extract in high fat diet-fed mice via enhanced gut butyrate production. European Journal of Nutrition, 57(8), pp. 2897-2911. doi:10.1007/s00394-017-1561-5.
Singh DP, et al. Co-supplementation of Isomalto-oligosaccharides Potentiates Metabolic Health Benefits of Polyphenol-rich Cranberry Extract in High Fat Diet-fed Mice Via Enhanced Gut Butyrate Production. Eur J Nutr. 2018;57(8):2897-2911. PubMed PMID: 29127476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-supplementation of isomalto-oligosaccharides potentiates metabolic health benefits of polyphenol-rich cranberry extract in high fat diet-fed mice via enhanced gut butyrate production. AU - Singh,Dhirendra Pratap, AU - Singh,Shashank, AU - Bijalwan,Vandana, AU - Kumar,Vijay, AU - Khare,Pragyanshu, AU - Baboota,Ritesh Kumar, AU - Singh,Paramdeep, AU - Boparai,Ravneet Kaur, AU - Singh,Jagdeep, AU - Kondepudi,Kanthi Kiran, AU - Chopra,Kanwaljit, AU - Bishnoi,Mahendra, Y1 - 2017/11/10/ PY - 2017/04/19/received PY - 2017/10/13/accepted PY - 2017/11/12/pubmed PY - 2019/4/26/medline PY - 2017/11/12/entrez KW - Butyrate KW - Cranberry extract KW - Ectopic fat KW - Isomalto-oligosaccharides KW - Mucin KW - Polyphenols SP - 2897 EP - 2911 JF - European journal of nutrition JO - Eur J Nutr VL - 57 IS - 8 N2 - PURPOSE: Cranberries are a rich source of polyphenolic antioxidants. Purified sugars or artificial sweeteners are being added to cranberry-based food products to mask tartness. Refined sugar and artificial sweeteners intake modulate gut microbiota and result in metabolic complications. We evaluated effects of isomalto-oligosaccharides (IMOs; sweet tasting non-digestible oligosaccharides) with cranberry extract (CRX) on high fat diet (HFD)-induced metabolic alterations in mice. METHODS: Male Swiss albino mice were fed normal chow or HFD (58% fat kcal), and were administered either CRX (200 mg/kg) alone or in combination with IMOs (1 g/kg). Cecal short-chain fatty acids, abundances of selected (1) butyrate producing, (2) metabolically beneficial, and (3) selective lipopolysaccharides producing gram negative gut bacteria were studied. Further, gut-related histological, biochemical, genomic changes along with circulating pro-/anti-inflammatory markers and systemic obesity-associated metabolic changes were studied. RESULTS: Co-supplementation of CRX and IMOs significantly improved cecal SCFAs, especially butyrate levels, selected butyrate-producing bacteria (clostridial cluster XIVa bacteria) and butyrate kinase expression in HFD-fed mice. The combination also significantly improved gut beneficial bacterial abundance, gut histology and related changes (colon mucin production, gut permeability) as compared to individual agents. It also prevented HFD-induced systemic and tissue inflammation, glucose intolerance and systemic obesity-associated metabolic changes in adipose tissue and liver. The combination of CRX and IMOs appeared more effective in the prevention of HFD-induced gut derangements. CONCLUSION: Combination of CRX and IMOs could be advantageous for normalization of metabolic alterations seen in diet-induced obesity via beneficial modulation of gastrointestinal health. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/29127476/Co_supplementation_of_isomalto_oligosaccharides_potentiates_metabolic_health_benefits_of_polyphenol_rich_cranberry_extract_in_high_fat_diet_fed_mice_via_enhanced_gut_butyrate_production_ L2 - https://dx.doi.org/10.1007/s00394-017-1561-5 DB - PRIME DP - Unbound Medicine ER -