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Iron-induced pro-oxidant and pro-lipogenic responses in relation to impaired synthesis and accretion of long-chain polyunsaturated fatty acids in rat hepatic and extrahepatic tissues.
Nutrition. 2018 Jan; 45:49-58.N

Abstract

OBJECTIVES

Iron is involved in processes involving oxygen transfer and utilization. Excess iron is linked to cardiovascular diseases and some types of cancer. Iron overload is associated with oxidative stress development, and may have important interactions with lipid metabolism in the liver favoring the development and progression of non-alcoholic fatty liver disease. The aim of the study described here was to assess the effect of high intake of iron on oxidative stress-related parameters, lipid metabolism, and levels of long-chain polyunsaturated fatty acids (LCPUFAs) in liver and other tissues of the rat.

METHODS

Male Wistar rats (21 d old) were fed an iron-rich diet (200 mg iron/kg diet, IRD) versus a control diet (50 mg iron/kg diet; CD) for 21 d. Samples of erythrocytes, liver, adipose tissue, brain, heart, and testicles were evaluated for fatty acid composition and hepatic biochemical and oxidative stress parameters, Δ-6 and Δ-5 desaturase activities, SREBP-1c and PPAR-α mRNA expression and DNA-binding capacity, and lipolytic, lipogenic, and antioxidant enzymatic activities.

RESULTS

The IRD caused liver steatosis and increased activity of plasma transaminases, with higher oxidative stress status in plasma and liver. Liver Δ-6 and Δ-5 desaturase exhibited decreased activity, but enhanced expression in response to the IRD compared with the CD, with lower levels of ω-3 and ω-6 LCPUFAs and higher expression and DNA binding of SREBP-1c, whereas expression and DNA-binding activity of PPAR-α were diminished.

CONCLUSIONS

IRD induced oxidative stress and a reduction in the desaturation capacity of the liver, with LCPUFA depletion in the different tissues studied, thus promoting a pro-steatotic condition in the liver.

Authors+Show Affiliations

Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile; Lipid Center, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile. Electronic address: rvalenzuelab@med.uchile.cl.Lipid Center, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.Medical Technology Department, Faculty of Medicine, University of Chile, Santiago, Chile.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.Nutrition and Dietetics School, Faculty of Health Sciences, Catholic University of Maule, Curicó, Chile.Lipid Center, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile.Molecular and Clinical Pharmacology Program, Institute of Biomedical Science, Faculty of Medicine, University of Chile, Santiago, Chile.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29129237

Citation

Valenzuela, Rodrigo, et al. "Iron-induced Pro-oxidant and Pro-lipogenic Responses in Relation to Impaired Synthesis and Accretion of Long-chain Polyunsaturated Fatty Acids in Rat Hepatic and Extrahepatic Tissues." Nutrition (Burbank, Los Angeles County, Calif.), vol. 45, 2018, pp. 49-58.
Valenzuela R, Rincón-Cervera MÁ, Echeverría F, et al. Iron-induced pro-oxidant and pro-lipogenic responses in relation to impaired synthesis and accretion of long-chain polyunsaturated fatty acids in rat hepatic and extrahepatic tissues. Nutrition. 2018;45:49-58.
Valenzuela, R., Rincón-Cervera, M. Á., Echeverría, F., Barrera, C., Espinosa, A., Hernández-Rodas, M. C., Ortiz, M., Valenzuela, A., & Videla, L. A. (2018). Iron-induced pro-oxidant and pro-lipogenic responses in relation to impaired synthesis and accretion of long-chain polyunsaturated fatty acids in rat hepatic and extrahepatic tissues. Nutrition (Burbank, Los Angeles County, Calif.), 45, 49-58. https://doi.org/10.1016/j.nut.2017.07.007
Valenzuela R, et al. Iron-induced Pro-oxidant and Pro-lipogenic Responses in Relation to Impaired Synthesis and Accretion of Long-chain Polyunsaturated Fatty Acids in Rat Hepatic and Extrahepatic Tissues. Nutrition. 2018;45:49-58. PubMed PMID: 29129237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron-induced pro-oxidant and pro-lipogenic responses in relation to impaired synthesis and accretion of long-chain polyunsaturated fatty acids in rat hepatic and extrahepatic tissues. AU - Valenzuela,Rodrigo, AU - Rincón-Cervera,Miguel Ángel, AU - Echeverría,Francisca, AU - Barrera,Cynthia, AU - Espinosa,Alejandra, AU - Hernández-Rodas,María Catalina, AU - Ortiz,Macarena, AU - Valenzuela,Alfonso, AU - Videla,Luis A, Y1 - 2017/07/17/ PY - 2017/04/07/received PY - 2017/07/04/revised PY - 2017/07/05/accepted PY - 2017/11/14/entrez PY - 2017/11/14/pubmed PY - 2018/7/18/medline KW - Iron KW - Liver Δ-6/Δ-5 desaturase activity KW - Oxidative stress KW - Tissue long-chain polyunsaturated fatty acid metabolism SP - 49 EP - 58 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 45 N2 - OBJECTIVES: Iron is involved in processes involving oxygen transfer and utilization. Excess iron is linked to cardiovascular diseases and some types of cancer. Iron overload is associated with oxidative stress development, and may have important interactions with lipid metabolism in the liver favoring the development and progression of non-alcoholic fatty liver disease. The aim of the study described here was to assess the effect of high intake of iron on oxidative stress-related parameters, lipid metabolism, and levels of long-chain polyunsaturated fatty acids (LCPUFAs) in liver and other tissues of the rat. METHODS: Male Wistar rats (21 d old) were fed an iron-rich diet (200 mg iron/kg diet, IRD) versus a control diet (50 mg iron/kg diet; CD) for 21 d. Samples of erythrocytes, liver, adipose tissue, brain, heart, and testicles were evaluated for fatty acid composition and hepatic biochemical and oxidative stress parameters, Δ-6 and Δ-5 desaturase activities, SREBP-1c and PPAR-α mRNA expression and DNA-binding capacity, and lipolytic, lipogenic, and antioxidant enzymatic activities. RESULTS: The IRD caused liver steatosis and increased activity of plasma transaminases, with higher oxidative stress status in plasma and liver. Liver Δ-6 and Δ-5 desaturase exhibited decreased activity, but enhanced expression in response to the IRD compared with the CD, with lower levels of ω-3 and ω-6 LCPUFAs and higher expression and DNA binding of SREBP-1c, whereas expression and DNA-binding activity of PPAR-α were diminished. CONCLUSIONS: IRD induced oxidative stress and a reduction in the desaturation capacity of the liver, with LCPUFA depletion in the different tissues studied, thus promoting a pro-steatotic condition in the liver. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/29129237/Iron_induced_pro_oxidant_and_pro_lipogenic_responses_in_relation_to_impaired_synthesis_and_accretion_of_long_chain_polyunsaturated_fatty_acids_in_rat_hepatic_and_extrahepatic_tissues_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(17)30153-3 DB - PRIME DP - Unbound Medicine ER -