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Effect of Native and Minimally Modified Low-density Lipoprotein on the Activation of Monocyte Subsets.
Arch Med Res. 2017 Jul; 48(5):432-440.AM

Abstract

BACKGROUND

In atherosclerosis, monocytes are essential and secrete pro-inflammatory cytokines in response to modified low-density lipoprotein (LDL). Human CD14++CD16-, CD14++CD16+ and CD14+CD16++ monocytes produce different cytokines. The objective of this research was to determine the number of monocyte subsets positives to cytokines in response to native (nLDL) and minimally modified LDL (mmLDL).

METHODS

Human monocytes from healthy individuals were purified by negative selection and were stimulated with nLDL, mmLDL or LPS. Subsequently, human total monocytes were incubated with monoclonal antibodies specific for CD14 or both CD14 and CD16 to characterize total monocytes and monocyte subsets and with antibodies specific to anti-tumor necrosis factor (TNF)-α, anti-interleukin (IL)-6 and anti-IL-10. The number of cells positive for cytokines was determined and cells cultured with nLDL, mmLDL and LPS were compared with cells cultured only with culture medium.

RESULTS

We found that nLDL does not induce in the total monocyte population or in the three monocyte subsets positives to cytokines. MmLDL induced in total monocytes positives to TNF-α and IL-6 as well as in both CD14++CD16+ and CD14+CD16++ and in CD14++CD16+ monocytes, respectively. Moreover, total monocytes and the three monocyte subsets expressed few amounts of cells positives to IL-10 in response to mmLDL.

CONCLUSION

Our study demonstrated that nLDL did not induce cells positives to cytokines and that the CD14++CD16+ and CD14+CD16++ monocyte subsets could be the main sources of TNF-α and IL-6, respectively, in response to mmLDL, which promotes the development and progression of atherosclerotic plaque.

Authors+Show Affiliations

Unidad de Investigación Médica en Inmunología, Unidad Médica de Alta Especialidad, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México.Unidad de Investigación Médica en Inmunología, Unidad Médica de Alta Especialidad, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México.Unidad de Investigación Médica en Inmunología, Unidad Médica de Alta Especialidad, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México.Coordinación de Educación en Salud, Instituto Mexicano del Seguro Social, Ciudad de México, México.Unidad de Investigación Médica en Inmunología, Unidad Médica de Alta Especialidad, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México. Electronic address: luischz@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29133194

Citation

Blanco-Favela, Francisco, et al. "Effect of Native and Minimally Modified Low-density Lipoprotein On the Activation of Monocyte Subsets." Archives of Medical Research, vol. 48, no. 5, 2017, pp. 432-440.
Blanco-Favela F, Espinosa-Luna JE, Chávez-Rueda AK, et al. Effect of Native and Minimally Modified Low-density Lipoprotein on the Activation of Monocyte Subsets. Arch Med Res. 2017;48(5):432-440.
Blanco-Favela, F., Espinosa-Luna, J. E., Chávez-Rueda, A. K., Madrid-Miller, A., & Chávez-Sánchez, L. (2017). Effect of Native and Minimally Modified Low-density Lipoprotein on the Activation of Monocyte Subsets. Archives of Medical Research, 48(5), 432-440. https://doi.org/10.1016/j.arcmed.2017.11.001
Blanco-Favela F, et al. Effect of Native and Minimally Modified Low-density Lipoprotein On the Activation of Monocyte Subsets. Arch Med Res. 2017;48(5):432-440. PubMed PMID: 29133194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Native and Minimally Modified Low-density Lipoprotein on the Activation of Monocyte Subsets. AU - Blanco-Favela,Francisco, AU - Espinosa-Luna,José Esteban, AU - Chávez-Rueda,Adriana Karina, AU - Madrid-Miller,Alejandra, AU - Chávez-Sánchez,Luis, Y1 - 2017/11/10/ PY - 2016/09/23/received PY - 2017/11/02/accepted PY - 2017/11/15/pubmed PY - 2017/12/21/medline PY - 2017/11/15/entrez KW - IL-6 KW - Monocyte subsets KW - TNF-α KW - mmLDL KW - nLDL SP - 432 EP - 440 JF - Archives of medical research JO - Arch. Med. Res. VL - 48 IS - 5 N2 - BACKGROUND: In atherosclerosis, monocytes are essential and secrete pro-inflammatory cytokines in response to modified low-density lipoprotein (LDL). Human CD14++CD16-, CD14++CD16+ and CD14+CD16++ monocytes produce different cytokines. The objective of this research was to determine the number of monocyte subsets positives to cytokines in response to native (nLDL) and minimally modified LDL (mmLDL). METHODS: Human monocytes from healthy individuals were purified by negative selection and were stimulated with nLDL, mmLDL or LPS. Subsequently, human total monocytes were incubated with monoclonal antibodies specific for CD14 or both CD14 and CD16 to characterize total monocytes and monocyte subsets and with antibodies specific to anti-tumor necrosis factor (TNF)-α, anti-interleukin (IL)-6 and anti-IL-10. The number of cells positive for cytokines was determined and cells cultured with nLDL, mmLDL and LPS were compared with cells cultured only with culture medium. RESULTS: We found that nLDL does not induce in the total monocyte population or in the three monocyte subsets positives to cytokines. MmLDL induced in total monocytes positives to TNF-α and IL-6 as well as in both CD14++CD16+ and CD14+CD16++ and in CD14++CD16+ monocytes, respectively. Moreover, total monocytes and the three monocyte subsets expressed few amounts of cells positives to IL-10 in response to mmLDL. CONCLUSION: Our study demonstrated that nLDL did not induce cells positives to cytokines and that the CD14++CD16+ and CD14+CD16++ monocyte subsets could be the main sources of TNF-α and IL-6, respectively, in response to mmLDL, which promotes the development and progression of atherosclerotic plaque. SN - 1873-5487 UR - https://www.unboundmedicine.com/medline/citation/29133194/Effect_of_Native_and_Minimally_Modified_Low_density_Lipoprotein_on_the_Activation_of_Monocyte_Subsets_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0188-4409(17)30208-4 DB - PRIME DP - Unbound Medicine ER -