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Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds.
Sci Rep. 2017 11 13; 7(1):15385.SR

Abstract

Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society.

Authors+Show Affiliations

Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900, Brasília, DF, Brazil. Universidade de Brasília, Departamento de Biologia Celular, Campus Universitário, 70910-900, Brasília, DF, Brazil.Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900, Brasília, DF, Brazil.Instituto Federal Goiano, Campus Urutaí, Laboratório de Biotecnologia, 75790-000, Urutaí, GO, Brazil.Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900, Brasília, DF, Brazil. Embrapa Agrossilvipastoril, Rod. dos Pioneiros MT-222, 78550-970, Sinop, MT, Brazil.Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900, Brasília, DF, Brazil. francisco.aragao@embrapa.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29133924

Citation

Sousa, Natália L., et al. "Bio-detoxification of Ricin in Castor Bean (Ricinus Communis L.) Seeds." Scientific Reports, vol. 7, no. 1, 2017, p. 15385.
Sousa NL, Cabral GB, Vieira PM, et al. Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds. Sci Rep. 2017;7(1):15385.
Sousa, N. L., Cabral, G. B., Vieira, P. M., Baldoni, A. B., & Aragão, F. J. L. (2017). Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds. Scientific Reports, 7(1), 15385. https://doi.org/10.1038/s41598-017-15636-7
Sousa NL, et al. Bio-detoxification of Ricin in Castor Bean (Ricinus Communis L.) Seeds. Sci Rep. 2017 11 13;7(1):15385. PubMed PMID: 29133924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds. AU - Sousa,Natália L, AU - Cabral,Glaucia B, AU - Vieira,Pabline M, AU - Baldoni,Aisy B, AU - Aragão,Francisco J L, Y1 - 2017/11/13/ PY - 2017/08/11/received PY - 2017/10/30/accepted PY - 2017/11/15/entrez PY - 2017/11/15/pubmed PY - 2019/7/4/medline SP - 15385 EP - 15385 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29133924/Bio_detoxification_of_ricin_in_castor_bean__Ricinus_communis_L___seeds_ L2 - https://doi.org/10.1038/s41598-017-15636-7 DB - PRIME DP - Unbound Medicine ER -