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Identification of membrane proteome of Paracoccidioides lutzii and its regulation by zinc.
Future Sci OA. 2017 Nov; 3(4):FSO232.FS

Abstract

Aim

During infection development in the host, Paracoccidioides spp. faces the deprivation of micronutrients, a mechanism called nutritional immunity. This condition induces the remodeling of proteins present in different metabolic pathways. Therefore, we attempted to identify membrane proteins and their regulation by zinc in Paracoccidioides lutzii.

Materials & methods

Membranes enriched fraction of yeast cells of P. lutzii were isolated, purified and identified by 2D LC-MS/MS detection and database search.

Results & conclusion

Zinc deprivation suppressed the expression of membrane proteins such as glycoproteins, those involved in cell wall synthesis and those related to oxidative phosphorylation. This is the first study describing membrane proteins and the effect of zinc deficiency in their regulation in one member of the genus Paracoccidioides.

Authors+Show Affiliations

Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Microscopia, Universidade de Brasília, Brasília, Brazil. Laboratório de Microscopia, Universidade de Brasília, Brasília, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29134119

Citation

de Curcio, Juliana Santana, et al. "Identification of Membrane Proteome of Paracoccidioides Lutzii and Its Regulation By Zinc." Future Science OA, vol. 3, no. 4, 2017, pp. FSO232.
de Curcio JS, Silva MG, Silva Bailão MG, et al. Identification of membrane proteome of Paracoccidioides lutzii and its regulation by zinc. Future Sci OA. 2017;3(4):FSO232.
de Curcio, J. S., Silva, M. G., Silva Bailão, M. G., Báo, S. N., Casaletti, L., Bailão, A. M., & de Almeida Soares, C. M. (2017). Identification of membrane proteome of Paracoccidioides lutzii and its regulation by zinc. Future Science OA, 3(4), FSO232. https://doi.org/10.4155/fsoa-2017-0044
de Curcio JS, et al. Identification of Membrane Proteome of Paracoccidioides Lutzii and Its Regulation By Zinc. Future Sci OA. 2017;3(4):FSO232. PubMed PMID: 29134119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of membrane proteome of Paracoccidioides lutzii and its regulation by zinc. AU - de Curcio,Juliana Santana, AU - Silva,Marielle Garcia, AU - Silva Bailão,Mirelle Garcia, AU - Báo,Sônia Nair, AU - Casaletti,Luciana, AU - Bailão,Alexandre Mello, AU - de Almeida Soares,Célia Maria, Y1 - 2017/07/25/ PY - 2017/04/06/received PY - 2017/06/21/accepted PY - 2017/11/15/entrez PY - 2017/11/15/pubmed PY - 2017/11/15/medline KW - Paracoccidioides lutzii KW - membranes KW - nanoUPLC-MSE KW - proteome KW - zinc deprivation SP - FSO232 EP - FSO232 JF - Future science OA JO - Future Sci OA VL - 3 IS - 4 N2 - Aim: During infection development in the host, Paracoccidioides spp. faces the deprivation of micronutrients, a mechanism called nutritional immunity. This condition induces the remodeling of proteins present in different metabolic pathways. Therefore, we attempted to identify membrane proteins and their regulation by zinc in Paracoccidioides lutzii. Materials & methods: Membranes enriched fraction of yeast cells of P. lutzii were isolated, purified and identified by 2D LC-MS/MS detection and database search. Results & conclusion: Zinc deprivation suppressed the expression of membrane proteins such as glycoproteins, those involved in cell wall synthesis and those related to oxidative phosphorylation. This is the first study describing membrane proteins and the effect of zinc deficiency in their regulation in one member of the genus Paracoccidioides. SN - 2056-5623 UR - https://www.unboundmedicine.com/medline/citation/29134119/Identification_of_membrane_proteome_of_Paracoccidioides_lutzii_and_its_regulation_by_zinc_ L2 - https://doi.org/10.4155/fsoa-2017-0044 DB - PRIME DP - Unbound Medicine ER -
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