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Rapid, directed transport of DC-SIGN clusters in the plasma membrane.
Sci Adv. 2017 11; 3(11):eaao1616.SA

Abstract

C-type lectins, including dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), are all-purpose pathogen receptors that exist in nanoclusters in plasma membranes of dendritic cells. A small fraction of these clusters, obvious from the videos, can undergo rapid, directed transport in the plane of the plasma membrane at average speeds of more than 1 μm/s in both dendritic cells and MX DC-SIGN murine fibroblasts ectopically expressing DC-SIGN. Surprisingly, instantaneous speeds can be considerably greater. In MX DC-SIGN cells, many cluster trajectories are colinear with microtubules that reside close to the ventral membrane, and the microtubule-depolymerizing drug, nocodazole, markedly reduced the areal density of directed movement trajectories, suggesting a microtubule motor-driven transport mechanism; by contrast, latrunculin A, which affects the actin network, did not depress this movement. Rapid, retrograde movement of DC-SIGN may be an efficient mechanism for bringing bound pathogen on the leading edge and projections of dendritic cells to the perinuclear region for internalization and processing. Dengue virus bound to DC-SIGN on dendritic projections was rapidly transported toward the cell center. The existence of this movement within the plasma membrane points to an unexpected lateral transport mechanism in mammalian cells and challenges our current concepts of cortex-membrane interactions.

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Authors+Show Affiliations

Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

MeSH

Actin CytoskeletonAnimalsBiological TransportBridged Bicyclo Compounds, HeterocyclicCell Adhesion MoleculesCell LineCell MembraneDendritic CellsDengue VirusFibroblastsHumansLectins, C-TypeMiceMicroscopy, ConfocalMicrotubulesNIH 3T3 CellsNocodazoleReceptors, Cell SurfaceThiazolidines

Pub Type(s)

Journal Article

Research Support, N.I.H., Extramural

Language

eng

PubMed ID

29134199

Citation

Liu, Ping, et al. "Rapid, Directed Transport of DC-SIGN Clusters in the Plasma Membrane." Science Advances, vol. 3, no. 11, 2017, pp. eaao1616.

Liu P, Weinreb V, Ridilla M, et al. Rapid, directed transport of DC-SIGN clusters in the plasma membrane. Sci Adv. 2017;3(11):eaao1616.

Liu, P., Weinreb, V., Ridilla, M., Betts, L., Patel, P., de Silva, A. M., Thompson, N. L., & Jacobson, K. (2017). Rapid, directed transport of DC-SIGN clusters in the plasma membrane. Science Advances, 3(11), eaao1616. https://doi.org/10.1126/sciadv.aao1616

Liu P, et al. Rapid, Directed Transport of DC-SIGN Clusters in the Plasma Membrane. Sci Adv. 2017;3(11):eaao1616. PubMed PMID: 29134199.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Rapid, directed transport of DC-SIGN clusters in the plasma membrane. AU - Liu,Ping, AU - Weinreb,Violetta, AU - Ridilla,Marc, AU - Betts,Laurie, AU - Patel,Pratik, AU - de Silva,Aravinda M, AU - Thompson,Nancy L, AU - Jacobson,Ken, Y1 - 2017/11/08/ PY - 2017/06/20/received PY - 2017/10/16/accepted PY - 2017/11/15/entrez PY - 2017/11/15/pubmed PY - 2019/6/30/medline SP - eaao1616 EP - eaao1616 JF - Science advances JO - Sci Adv VL - 3 IS - 11 N2 - C-type lectins, including dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), are all-purpose pathogen receptors that exist in nanoclusters in plasma membranes of dendritic cells. A small fraction of these clusters, obvious from the videos, can undergo rapid, directed transport in the plane of the plasma membrane at average speeds of more than 1 μm/s in both dendritic cells and MX DC-SIGN murine fibroblasts ectopically expressing DC-SIGN. Surprisingly, instantaneous speeds can be considerably greater. In MX DC-SIGN cells, many cluster trajectories are colinear with microtubules that reside close to the ventral membrane, and the microtubule-depolymerizing drug, nocodazole, markedly reduced the areal density of directed movement trajectories, suggesting a microtubule motor-driven transport mechanism; by contrast, latrunculin A, which affects the actin network, did not depress this movement. Rapid, retrograde movement of DC-SIGN may be an efficient mechanism for bringing bound pathogen on the leading edge and projections of dendritic cells to the perinuclear region for internalization and processing. Dengue virus bound to DC-SIGN on dendritic projections was rapidly transported toward the cell center. The existence of this movement within the plasma membrane points to an unexpected lateral transport mechanism in mammalian cells and challenges our current concepts of cortex-membrane interactions. SN - 2375-2548 UR - https://www.unboundmedicine.com/medline/citation/29134199/Rapid_directed_transport_of_DC_SIGN_clusters_in_the_plasma_membrane_ DB - PRIME DP - Unbound Medicine ER -