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Improving Quality of Banked Milk: Utility of Dornic Acid Test.
Indian J Pediatr. 2018 04; 85(4):272-275.IJ

Abstract

OBJECTIVE

To identify, if Dornic acid test done on human milk bank sample is as effective as doing routine culture, both pre and post pasteurization.

METHODS

The authors analyzed 477 samples, both pre and post pasteurization. Dornic acid measurement was performed by using N/9 NaOH and titrated to get dornicity of the sample. Senstivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) at different Dornic acid levels were calculated. To find diagnostic power of this study, ROC curve was prepared. Dornic acid for paired pre and post pasteurization were noted and analysed for statistical significance.

RESULTS

A significant growth was seen in 21% (98/477) samples with coagulase negative staphylococci and gram negative organisms being the major contaminants seen prior to pasteurization. In the index study, Dornic acidity ≥4°D had a sensitivity of 98% but very low specificity of just over 10%. However, 8°D had 92% specificity and acceptable sensitivity of 72% in comparison to gold standard microbiological criteria, making it a good test for analyzing the quality of milk before pasteurization.

CONCLUSIONS

Dornic acid test at 8°D has a very high specificity with acceptable sensitivity in comparison to dornicity at 4°D. It can be used as a simple method to select better quality of milk sample prior to pasteurization.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Bhisikar S
Department of Neonatology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Room no 123, 1st Floor, College Building, Sion (West), Mumbai, 400022, India. swapbhi@gmail.com.
Mondkar J
Department of Neonatology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Room no 123, 1st Floor, College Building, Sion (West), Mumbai, 400022, India.
Manerkar S
Department of Neonatology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Room no 123, 1st Floor, College Building, Sion (West), Mumbai, 400022, India.
Goel S
Department of Neonatology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Room no 123, 1st Floor, College Building, Sion (West), Mumbai, 400022, India.
D'Dsouza D
Department of Microbiology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Mumbai, India.
Baveja S
Department of Microbiology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Mumbai, India.

MeSH

HumansMilk BanksMilk, HumanPasteurizationQuality Control

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29139066

Citation

Bhisikar, Swapnil, et al. "Improving Quality of Banked Milk: Utility of Dornic Acid Test." Indian Journal of Pediatrics, vol. 85, no. 4, 2018, pp. 272-275.
Bhisikar S, Mondkar J, Manerkar S, et al. Improving Quality of Banked Milk: Utility of Dornic Acid Test. Indian J Pediatr. 2018;85(4):272-275.
Bhisikar, S., Mondkar, J., Manerkar, S., Goel, S., D'Dsouza, D., & Baveja, S. (2018). Improving Quality of Banked Milk: Utility of Dornic Acid Test. Indian Journal of Pediatrics, 85(4), 272-275. https://doi.org/10.1007/s12098-017-2518-7
Bhisikar S, et al. Improving Quality of Banked Milk: Utility of Dornic Acid Test. Indian J Pediatr. 2018;85(4):272-275. PubMed PMID: 29139066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improving Quality of Banked Milk: Utility of Dornic Acid Test. AU - Bhisikar,Swapnil, AU - Mondkar,Jayashree, AU - Manerkar,Swati, AU - Goel,Sorabh, AU - D'Dsouza,Desma, AU - Baveja,Sujata, Y1 - 2017/11/15/ PY - 2017/03/25/received PY - 2017/09/27/accepted PY - 2017/11/16/pubmed PY - 2018/12/12/medline PY - 2017/11/16/entrez KW - Dornic acid KW - Human milk bank KW - Pasteurization SP - 272 EP - 275 JF - Indian journal of pediatrics JO - Indian J Pediatr VL - 85 IS - 4 N2 - OBJECTIVE: To identify, if Dornic acid test done on human milk bank sample is as effective as doing routine culture, both pre and post pasteurization. METHODS: The authors analyzed 477 samples, both pre and post pasteurization. Dornic acid measurement was performed by using N/9 NaOH and titrated to get dornicity of the sample. Senstivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) at different Dornic acid levels were calculated. To find diagnostic power of this study, ROC curve was prepared. Dornic acid for paired pre and post pasteurization were noted and analysed for statistical significance. RESULTS: A significant growth was seen in 21% (98/477) samples with coagulase negative staphylococci and gram negative organisms being the major contaminants seen prior to pasteurization. In the index study, Dornic acidity ≥4°D had a sensitivity of 98% but very low specificity of just over 10%. However, 8°D had 92% specificity and acceptable sensitivity of 72% in comparison to gold standard microbiological criteria, making it a good test for analyzing the quality of milk before pasteurization. CONCLUSIONS: Dornic acid test at 8°D has a very high specificity with acceptable sensitivity in comparison to dornicity at 4°D. It can be used as a simple method to select better quality of milk sample prior to pasteurization. SN - 0973-7693 UR - https://www.unboundmedicine.com/medline/citation/29139066/Improving_Quality_of_Banked_Milk:_Utility_of_Dornic_Acid_Test_ DB - PRIME DP - Unbound Medicine ER -