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In vitro Protoscolicidal Effects of Cinnamomum zeylanicum Essential Oil and Its Toxicity in Mice.
Pharmacogn Mag 2017; 13(Suppl 3):S652-S657PM

Abstract

Background

This study investigates the scolicidal effects of Cinnamomum zeylanicum essential oil against the protoscoleces of hydatid cysts and its toxicity in the mice model.

Materials and Methods

Gas chromatography/mass spectroscopy analyses were used to identify the constituents of essential oil. Protoscoleces were treated with different concentrations of the essential oil (6.25-100 µL/mL) in each test tube for 5-30 min. The viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oil (0.5-4 mL/kg).

Results

The main components were found to be cinnamaldehyde (91.8%), ρ metoxicinamate (1.57%), and α pinene (1.25%). Findings indicate that C. zeylanicum essential oil with the concentrations of 100 and 50 µL/mL killed 100% of protoscoleces after 5 min of exposure. Also, the lower concentrations of C. zeylanicum essential oil motivated a late protoscolicidal effect. The LD50 value of intraperitoneal injection of C. zeylanicum essential oil was 2.07 mL/kg body weight after 48 h, and the maximum nonfatal dose was 1.52 mL/kg body weight. The results also showed that there was no significant toxicity following oral administration of C. zeylanicum essential oil for 2 weeks.

Conclusion

The results exhibited the favorable scolicidal activity of C. zeylanicum, which could be applied as a natural scolicidal agent in hydatid cyst surgery.

SUMMARY

We evaluated the efficacy of Cinnamomum zeylanicum essential oil against hydatid cyst protoscolecesThe viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining)Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oilC. zeylanicum with potent scolicidal activity could be applied as a natural scolicidal agent in surgery. Abbreviations used: GC/MS: Gas chromatography/mass spectrometry analysis; CE: Cystic echinococcosis; LD50: Lethal dose 50%; I.p: Intraperitoneally.

Authors+Show Affiliations

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.Department of Laboratory Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.Bam University of Medical Sciences, Bam, Iran. Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29142428

Citation

Mahmoudvand, Hossein, et al. "In Vitro Protoscolicidal Effects of Cinnamomum Zeylanicum Essential Oil and Its Toxicity in Mice." Pharmacognosy Magazine, vol. 13, no. Suppl 3, 2017, pp. S652-S657.
Mahmoudvand H, Mahmoudvand H, Oliaee RT, et al. In vitro Protoscolicidal Effects of Cinnamomum zeylanicum Essential Oil and Its Toxicity in Mice. Pharmacogn Mag. 2017;13(Suppl 3):S652-S657.
Mahmoudvand, H., Mahmoudvand, H., Oliaee, R. T., Kareshk, A. T., Mirbadie, S. R., & Aflatoonian, M. R. (2017). In vitro Protoscolicidal Effects of Cinnamomum zeylanicum Essential Oil and Its Toxicity in Mice. Pharmacognosy Magazine, 13(Suppl 3), pp. S652-S657. doi:10.4103/pm.pm_280_16.
Mahmoudvand H, et al. In Vitro Protoscolicidal Effects of Cinnamomum Zeylanicum Essential Oil and Its Toxicity in Mice. Pharmacogn Mag. 2017;13(Suppl 3):S652-S657. PubMed PMID: 29142428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro Protoscolicidal Effects of Cinnamomum zeylanicum Essential Oil and Its Toxicity in Mice. AU - Mahmoudvand,Hossein, AU - Mahmoudvand,Hormoz, AU - Oliaee,Razieh Tavakoli, AU - Kareshk,Amir Tavakoli, AU - Mirbadie,Seyed Reza, AU - Aflatoonian,Mohammad Reza, Y1 - 2017/10/11/ PY - 2016/06/09/received PY - 2016/07/18/revised PY - 2017/11/17/entrez PY - 2017/11/17/pubmed PY - 2017/11/17/medline KW - Cystic echinococcosis KW - gas chromatography/mass spectroscopy KW - hydatid cyst KW - protoscoleces SP - S652 EP - S657 JF - Pharmacognosy magazine JO - Pharmacogn Mag VL - 13 IS - Suppl 3 N2 - Background: This study investigates the scolicidal effects of Cinnamomum zeylanicum essential oil against the protoscoleces of hydatid cysts and its toxicity in the mice model. Materials and Methods: Gas chromatography/mass spectroscopy analyses were used to identify the constituents of essential oil. Protoscoleces were treated with different concentrations of the essential oil (6.25-100 µL/mL) in each test tube for 5-30 min. The viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oil (0.5-4 mL/kg). Results: The main components were found to be cinnamaldehyde (91.8%), ρ metoxicinamate (1.57%), and α pinene (1.25%). Findings indicate that C. zeylanicum essential oil with the concentrations of 100 and 50 µL/mL killed 100% of protoscoleces after 5 min of exposure. Also, the lower concentrations of C. zeylanicum essential oil motivated a late protoscolicidal effect. The LD50 value of intraperitoneal injection of C. zeylanicum essential oil was 2.07 mL/kg body weight after 48 h, and the maximum nonfatal dose was 1.52 mL/kg body weight. The results also showed that there was no significant toxicity following oral administration of C. zeylanicum essential oil for 2 weeks. Conclusion: The results exhibited the favorable scolicidal activity of C. zeylanicum, which could be applied as a natural scolicidal agent in hydatid cyst surgery. SUMMARY: We evaluated the efficacy of Cinnamomum zeylanicum essential oil against hydatid cyst protoscolecesThe viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining)Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oilC. zeylanicum with potent scolicidal activity could be applied as a natural scolicidal agent in surgery. Abbreviations used: GC/MS: Gas chromatography/mass spectrometry analysis; CE: Cystic echinococcosis; LD50: Lethal dose 50%; I.p: Intraperitoneally. SN - 0973-1296 UR - https://www.unboundmedicine.com/medline/citation/29142428/In_vitro_Protoscolicidal_Effects_of_Cinnamomum_zeylanicum_Essential_Oil_and_Its_Toxicity_in_Mice_ L2 - http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=51;spage=652;epage=657;aulast=Mahmoudvand DB - PRIME DP - Unbound Medicine ER -