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Circadian gene methylation in rotating-shift nurses: a cross-sectional study.
Chronobiol Int. 2018 01; 35(1):111-121.CI

Abstract

Investigating the methylation status of the circadian genes may contribute to a better understanding of the shift work-related circadian disruption in individuals exposed to artificial light at night. In the present study, we determined the methylation status of the circadian genes associated with a shift work pattern among nurses and midwives participating in a cross-sectional study in Lodz, Poland. Quantitative methylation polymerase chain reaction assays were used to assess promoter CpG methylation in PER1, PER2, PER3, CRY1, CRY2, BMAL1, CLOCK, and NPAS2 in genomic DNA from whole blood of 347 women having a rotating-shift work schedule and 363 women working days only. The percentage of methylated reference (PMR) was assessed using fluorescent probes for PER1, PER2, PER3, CRY1, and NPAS2, and the percentage of gene methylation, as the methylation index (MI), using two sets of primers for BMAL1, CLOCK, and CRY2. We tested the possible association between current and lifetime rotating night-shift work characteristics and circadian gene methylation by using proportional odds regression model with blood DNA methylation, categorized into tertiles, and adjusted for age, current smoking status, folate intake and blood collection time. The findings indicated that CpG methylation in PER2 promoter was significantly decreased (P < 0.004) among nurses and midwives currently working rotating shifts, as compared with day-working nurses and midwives. The lower percentage of PER2 methylation was associated with a higher monthly frequency of current night duties (2-7 night shifts, and eight or more night shifts per month) (P = 0.012) and was associated at borderline significance (P = 0.092) with the lifetime duration of shift work (>10 ≤ 20 years and >20 ≤ 43 years of rotating-shift work) among nurses and midwives (N = 710). Moreover, women with a longer lifetime duration of shift work presented a lower status of PER1 methylation (P = 0.040) than did the women with up to 10 years of rotating-shift work. Long lifetime duration of shift work (> 10 years) among current rotating night-shift workers (N = 347) was associated with BMAL1 hypomethylation (P = 0.013). Among eight of the investigated circadian genes, only PER1, PER2, and BMAL1 showed differential methylation attributable to the rotating-shift work of nurses and midwives. The findings on blood-based DNA methylation in the circadian genes may provide a better insight into the mechanistic principles underlying the possible health effects of night-shift work but these should be verified in further studies recruiting larger populations of shift workers.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Reszka E
a Department of Molecular Genetics and Epigenetics , Nofer Institute of Occupational Medicine , Lodz , Poland.
Wieczorek E
a Department of Molecular Genetics and Epigenetics , Nofer Institute of Occupational Medicine , Lodz , Poland.
Przybek M
a Department of Molecular Genetics and Epigenetics , Nofer Institute of Occupational Medicine , Lodz , Poland.
Jabłońska E
a Department of Molecular Genetics and Epigenetics , Nofer Institute of Occupational Medicine , Lodz , Poland.
Kałużny P
b Department of Environmental Epidemiology , Nofer Institute of Occupational Medicine , Lodz , Poland.
Bukowska-Damska A
b Department of Environmental Epidemiology , Nofer Institute of Occupational Medicine , Lodz , Poland.
Zienolddiny S
c Section of Toxicology and Biological Work Environment, Department of Biological and Chemical Work Environment , National Institute of Occupational Health , Oslo , Norway.
Pepłońska B
b Department of Environmental Epidemiology , Nofer Institute of Occupational Medicine , Lodz , Poland.

MeSH

AdultCircadian RhythmCross-Sectional StudiesFemaleHumansMethylationMiddle AgedNursesPolandTime FactorsWork Schedule Tolerance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29144171

Citation

Reszka, Edyta, et al. "Circadian Gene Methylation in Rotating-shift Nurses: a Cross-sectional Study." Chronobiology International, vol. 35, no. 1, 2018, pp. 111-121.
Reszka E, Wieczorek E, Przybek M, et al. Circadian gene methylation in rotating-shift nurses: a cross-sectional study. Chronobiol Int. 2018;35(1):111-121.
Reszka, E., Wieczorek, E., Przybek, M., Jabłońska, E., Kałużny, P., Bukowska-Damska, A., Zienolddiny, S., & Pepłońska, B. (2018). Circadian gene methylation in rotating-shift nurses: a cross-sectional study. Chronobiology International, 35(1), 111-121. https://doi.org/10.1080/07420528.2017.1388252
Reszka E, et al. Circadian Gene Methylation in Rotating-shift Nurses: a Cross-sectional Study. Chronobiol Int. 2018;35(1):111-121. PubMed PMID: 29144171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circadian gene methylation in rotating-shift nurses: a cross-sectional study. AU - Reszka,Edyta, AU - Wieczorek,Edyta, AU - Przybek,Monika, AU - Jabłońska,Ewa, AU - Kałużny,Paweł, AU - Bukowska-Damska,Agnieszka, AU - Zienolddiny,Shanbeh, AU - Pepłońska,Beata, Y1 - 2017/11/16/ PY - 2017/11/17/pubmed PY - 2019/3/22/medline PY - 2017/11/17/entrez KW - Circadian genes KW - DNA methylation KW - blood KW - nurses KW - rotating-shift work SP - 111 EP - 121 JF - Chronobiology international JO - Chronobiol Int VL - 35 IS - 1 N2 - Investigating the methylation status of the circadian genes may contribute to a better understanding of the shift work-related circadian disruption in individuals exposed to artificial light at night. In the present study, we determined the methylation status of the circadian genes associated with a shift work pattern among nurses and midwives participating in a cross-sectional study in Lodz, Poland. Quantitative methylation polymerase chain reaction assays were used to assess promoter CpG methylation in PER1, PER2, PER3, CRY1, CRY2, BMAL1, CLOCK, and NPAS2 in genomic DNA from whole blood of 347 women having a rotating-shift work schedule and 363 women working days only. The percentage of methylated reference (PMR) was assessed using fluorescent probes for PER1, PER2, PER3, CRY1, and NPAS2, and the percentage of gene methylation, as the methylation index (MI), using two sets of primers for BMAL1, CLOCK, and CRY2. We tested the possible association between current and lifetime rotating night-shift work characteristics and circadian gene methylation by using proportional odds regression model with blood DNA methylation, categorized into tertiles, and adjusted for age, current smoking status, folate intake and blood collection time. The findings indicated that CpG methylation in PER2 promoter was significantly decreased (P < 0.004) among nurses and midwives currently working rotating shifts, as compared with day-working nurses and midwives. The lower percentage of PER2 methylation was associated with a higher monthly frequency of current night duties (2-7 night shifts, and eight or more night shifts per month) (P = 0.012) and was associated at borderline significance (P = 0.092) with the lifetime duration of shift work (>10 ≤ 20 years and >20 ≤ 43 years of rotating-shift work) among nurses and midwives (N = 710). Moreover, women with a longer lifetime duration of shift work presented a lower status of PER1 methylation (P = 0.040) than did the women with up to 10 years of rotating-shift work. Long lifetime duration of shift work (> 10 years) among current rotating night-shift workers (N = 347) was associated with BMAL1 hypomethylation (P = 0.013). Among eight of the investigated circadian genes, only PER1, PER2, and BMAL1 showed differential methylation attributable to the rotating-shift work of nurses and midwives. The findings on blood-based DNA methylation in the circadian genes may provide a better insight into the mechanistic principles underlying the possible health effects of night-shift work but these should be verified in further studies recruiting larger populations of shift workers. SN - 1525-6073 UR - https://www.unboundmedicine.com/medline/citation/29144171/Circadian_gene_methylation_in_rotating_shift_nurses:_a_cross_sectional_study_ DB - PRIME DP - Unbound Medicine ER -