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Antibacterial, antibiofilm and antiquorum sensing effects of Thymus daenensis and Satureja hortensis essential oils against Staphylococcus aureus isolates.
J Appl Microbiol. 2018 Feb; 124(2):379-388.JA

Abstract

AIMS

The present study was conducted to investigate the effects of Thymus daenensis and Satureja hortensis essential oils (EOs) on the planktonic growth, biofilm formation and quorum sensing (QS) of some Staphylococcus aureus isolates (strong biofilm producers).

METHODS AND RESULTS

Minimum inhibitory concentration (MIC) of the EOs, inhibition of biofilm formation as well as disruption of preformed Staph. aureus biofilms were assessed. The antibiofilm activity of the EOs was determined using microtitre plate test (MtP) and scanning electron microscope (SEM). The QS inhibitory activity was also examined on the pregrown biofilms by gene expression analysis using quantitative real-time RT-polymerase chain reaction (PCR) of hld gene (RNAIII transcript). Moreover, tetrazolium-based colorimetric assay (MTT) was performed to detect cytotoxic effects of these EOs on the Vero cell line. Finally, the major components of the tested EOs were determined using Gas Chromatography-Mass Spectrometry (GC-MS). The MICs of T. daenensis and S. hortensis EOs against planktonic cells of the isolates were 0·0625 and 0·125 μl ml-1 respectively. The minimum bactericidal concentrations for both of the EOs was 0·125 μl ml-1 . The MtP test showed a significant inhibitory effect of the EOs on the biofilm formation and disruption at sub-MIC concentrations. These results were confirmed by SEM. Real-time PCR revealed a significant down-regulation of hld gene following treatment with MIC/2 concentration of S. hortensis EO. GC-MS analysis showed that carvacrol, terpinene and thymol were the major components of the applied EOs.

CONCLUSIONS

As selected EOs did not show significant cytotoxic effects even up to tenfold of MIC concentration, the applied EOs seem to be good candidates for preventing of biofilm formation of Staph. aureus cells.

SIGNIFICANCE AND IMPACT OF THE STUDY

The present study introduced T. daenensis and S. hortensis EOs as new antibiofilm, and S. hortensis EO as anti-QS herbal agents with natural origin against Staph. aureus.

Authors+Show Affiliations

Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29144601

Citation

Sharifi, A, et al. "Antibacterial, Antibiofilm and Antiquorum Sensing Effects of Thymus Daenensis and Satureja Hortensis Essential Oils Against Staphylococcus Aureus Isolates." Journal of Applied Microbiology, vol. 124, no. 2, 2018, pp. 379-388.
Sharifi A, Mohammadzadeh A, Zahraei Salehi T, et al. Antibacterial, antibiofilm and antiquorum sensing effects of Thymus daenensis and Satureja hortensis essential oils against Staphylococcus aureus isolates. J Appl Microbiol. 2018;124(2):379-388.
Sharifi, A., Mohammadzadeh, A., Zahraei Salehi, T., & Mahmoodi, P. (2018). Antibacterial, antibiofilm and antiquorum sensing effects of Thymus daenensis and Satureja hortensis essential oils against Staphylococcus aureus isolates. Journal of Applied Microbiology, 124(2), 379-388. https://doi.org/10.1111/jam.13639
Sharifi A, et al. Antibacterial, Antibiofilm and Antiquorum Sensing Effects of Thymus Daenensis and Satureja Hortensis Essential Oils Against Staphylococcus Aureus Isolates. J Appl Microbiol. 2018;124(2):379-388. PubMed PMID: 29144601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial, antibiofilm and antiquorum sensing effects of Thymus daenensis and Satureja hortensis essential oils against Staphylococcus aureus isolates. AU - Sharifi,A, AU - Mohammadzadeh,A, AU - Zahraei Salehi,T, AU - Mahmoodi,P, Y1 - 2018/01/11/ PY - 2017/07/21/received PY - 2017/10/10/revised PY - 2017/11/02/accepted PY - 2017/11/17/pubmed PY - 2018/5/15/medline PY - 2017/11/17/entrez KW - Staphylococcus aureus KW - antimicrobial KW - biofilm KW - gene expression KW - quorum sensing SP - 379 EP - 388 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 124 IS - 2 N2 - AIMS: The present study was conducted to investigate the effects of Thymus daenensis and Satureja hortensis essential oils (EOs) on the planktonic growth, biofilm formation and quorum sensing (QS) of some Staphylococcus aureus isolates (strong biofilm producers). METHODS AND RESULTS: Minimum inhibitory concentration (MIC) of the EOs, inhibition of biofilm formation as well as disruption of preformed Staph. aureus biofilms were assessed. The antibiofilm activity of the EOs was determined using microtitre plate test (MtP) and scanning electron microscope (SEM). The QS inhibitory activity was also examined on the pregrown biofilms by gene expression analysis using quantitative real-time RT-polymerase chain reaction (PCR) of hld gene (RNAIII transcript). Moreover, tetrazolium-based colorimetric assay (MTT) was performed to detect cytotoxic effects of these EOs on the Vero cell line. Finally, the major components of the tested EOs were determined using Gas Chromatography-Mass Spectrometry (GC-MS). The MICs of T. daenensis and S. hortensis EOs against planktonic cells of the isolates were 0·0625 and 0·125 μl ml-1 respectively. The minimum bactericidal concentrations for both of the EOs was 0·125 μl ml-1 . The MtP test showed a significant inhibitory effect of the EOs on the biofilm formation and disruption at sub-MIC concentrations. These results were confirmed by SEM. Real-time PCR revealed a significant down-regulation of hld gene following treatment with MIC/2 concentration of S. hortensis EO. GC-MS analysis showed that carvacrol, terpinene and thymol were the major components of the applied EOs. CONCLUSIONS: As selected EOs did not show significant cytotoxic effects even up to tenfold of MIC concentration, the applied EOs seem to be good candidates for preventing of biofilm formation of Staph. aureus cells. SIGNIFICANCE AND IMPACT OF THE STUDY: The present study introduced T. daenensis and S. hortensis EOs as new antibiofilm, and S. hortensis EO as anti-QS herbal agents with natural origin against Staph. aureus. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/29144601/Antibacterial_antibiofilm_and_antiquorum_sensing_effects_of_Thymus_daenensis_and_Satureja_hortensis_essential_oils_against_Staphylococcus_aureus_isolates_ L2 - https://doi.org/10.1111/jam.13639 DB - PRIME DP - Unbound Medicine ER -