Tags

Type your tag names separated by a space and hit enter

Up-regulation of miR-299 suppressed the invasion and migration of HTR-8/SVneo trophoblast cells partly via targeting HDAC2 in pre-eclampsia.
Biomed Pharmacother 2018; 97:1222-1228BP

Abstract

Pre-eclampsia (PE), a pregnancy-associated disorder, is a major contributor to maternal mortality and morbidity worldwide. Recently, microRNAs (miRNAs) were found to be associated with the pathogenesis of PE. The present study investigated the function of miR-299 in HTR-8/SVneo trophoblast cells and explored its underlying mechanism in the pathogenesis of PE. The miR-299 and histone deacetylase 2 (HDAC2) mRNA expression levels were determined by quantitative real-time PCR. Transwell invasion and wound healing assays were used to measure cell invasive and migratory ability. Luciferase reporter assay was performed to confirm the downstream targets of miR-299. Western blot was performed to measure the protein expression of HDAC2. The expression level of miR-299 in placental tissues from pregnant women with severe PE was significantly higher than that from normal pregnant women. MiR-299 overexpression suppressed the invasion and migration of trophoblast cells; and knock-down of miR-299 promoted the invasion and migration of trophoblast cells. Bioinformatics prediction and luciferase reporter assay confirmed that miR-299 directly targeted the 3' untranslated region of HDAC2. The mRNA expression level of HDAC2 in placental tissues from pregnant women with severe PE was significantly lower than that from normal pregnant women, and was negatively correlated the expression level of miR-299 in placental tissues from pregnant women with severe PE. HDAC2 siRNA transfection suppressed the mRNA and protein expression levels of HDAC in trophoblast cells compared with control group, and HDAC2 siRNA transfection also suppressed the trophoblast cell invasion and migration compared with control group. Enforced expression of HDAC2 in trophoblast cells attenuated the inhibitory effects of miR-299 overexpression on cell invasion and migration. In summary, the results showed the up-regulation of miR-299 in the placental tissues from women with severe PE and miR-299 suppressed the invasion and migration of trophoblast cells partly via targeting HDAC2.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, China.Department of Obstetrics and Gynecology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, China.Department of Obstetrics and Gynecology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, China.Department of Obstetrics and Gynecology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, China.Department of Obstetrics and Gynecology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, China. Electronic address: zhanghaieagle@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29145147

Citation

Gao, Yu, et al. "Up-regulation of miR-299 Suppressed the Invasion and Migration of HTR-8/SVneo Trophoblast Cells Partly Via Targeting HDAC2 in Pre-eclampsia." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 97, 2018, pp. 1222-1228.
Gao Y, She R, Wang Q, et al. Up-regulation of miR-299 suppressed the invasion and migration of HTR-8/SVneo trophoblast cells partly via targeting HDAC2 in pre-eclampsia. Biomed Pharmacother. 2018;97:1222-1228.
Gao, Y., She, R., Wang, Q., Li, Y., & Zhang, H. (2018). Up-regulation of miR-299 suppressed the invasion and migration of HTR-8/SVneo trophoblast cells partly via targeting HDAC2 in pre-eclampsia. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 97, pp. 1222-1228. doi:10.1016/j.biopha.2017.11.053.
Gao Y, et al. Up-regulation of miR-299 Suppressed the Invasion and Migration of HTR-8/SVneo Trophoblast Cells Partly Via Targeting HDAC2 in Pre-eclampsia. Biomed Pharmacother. 2018;97:1222-1228. PubMed PMID: 29145147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Up-regulation of miR-299 suppressed the invasion and migration of HTR-8/SVneo trophoblast cells partly via targeting HDAC2 in pre-eclampsia. AU - Gao,Yu, AU - She,Ruilian, AU - Wang,Qian, AU - Li,Yan, AU - Zhang,Haiying, Y1 - 2017/11/13/ PY - 2017/09/16/received PY - 2017/11/07/revised PY - 2017/11/07/accepted PY - 2017/11/18/pubmed PY - 2018/8/8/medline PY - 2017/11/18/entrez KW - HDAC2 KW - Invasion KW - MiR-299 KW - Migration KW - Pre-eclampsia KW - Trophoblast SP - 1222 EP - 1228 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 97 N2 - Pre-eclampsia (PE), a pregnancy-associated disorder, is a major contributor to maternal mortality and morbidity worldwide. Recently, microRNAs (miRNAs) were found to be associated with the pathogenesis of PE. The present study investigated the function of miR-299 in HTR-8/SVneo trophoblast cells and explored its underlying mechanism in the pathogenesis of PE. The miR-299 and histone deacetylase 2 (HDAC2) mRNA expression levels were determined by quantitative real-time PCR. Transwell invasion and wound healing assays were used to measure cell invasive and migratory ability. Luciferase reporter assay was performed to confirm the downstream targets of miR-299. Western blot was performed to measure the protein expression of HDAC2. The expression level of miR-299 in placental tissues from pregnant women with severe PE was significantly higher than that from normal pregnant women. MiR-299 overexpression suppressed the invasion and migration of trophoblast cells; and knock-down of miR-299 promoted the invasion and migration of trophoblast cells. Bioinformatics prediction and luciferase reporter assay confirmed that miR-299 directly targeted the 3' untranslated region of HDAC2. The mRNA expression level of HDAC2 in placental tissues from pregnant women with severe PE was significantly lower than that from normal pregnant women, and was negatively correlated the expression level of miR-299 in placental tissues from pregnant women with severe PE. HDAC2 siRNA transfection suppressed the mRNA and protein expression levels of HDAC in trophoblast cells compared with control group, and HDAC2 siRNA transfection also suppressed the trophoblast cell invasion and migration compared with control group. Enforced expression of HDAC2 in trophoblast cells attenuated the inhibitory effects of miR-299 overexpression on cell invasion and migration. In summary, the results showed the up-regulation of miR-299 in the placental tissues from women with severe PE and miR-299 suppressed the invasion and migration of trophoblast cells partly via targeting HDAC2. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/29145147/Up_regulation_of_miR_299_suppressed_the_invasion_and_migration_of_HTR_8/SVneo_trophoblast_cells_partly_via_targeting_HDAC2_in_pre_eclampsia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)34797-2 DB - PRIME DP - Unbound Medicine ER -