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GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon.
Theor Appl Genet. 2018 Mar; 131(3):569-579.TA

Abstract

KEY MESSAGE

Map-based cloning identified CmGL that encodes a HD-ZIP type IV transcription factor that controls multicellular trichome initiation in melon. Trichomes are small hairs covering the aerial parts of plants that originate from the epidermal cells, which can protect plants against the damage by insects and pathogens. The regulatory pathway of unicellular trichomes has been well studied in the model plant Arabidopsis. Little is known about the genetic control and regulation of trichome development in melon (Cucumis melo L.) which has multicellular trichomes. In this study, we identified a melon mutant, cmgl, which showed completely glabrous on all aerial organs. A bulked segregant analysis was conducted to identify polymorphic markers for linkage analysis in a population with 256 F2 plants, which allowed to locate the cmgl locus in melon chromosome VIII. Next-generation sequencing-aided marker discovery and fine mapping in a large population with 1536 F2 plants narrowed the candidate gene region to 12 kb that harbored only one candidate gene for cmgl, which encoded a class IV homeodomain-associated leucine zipper transcription factor. Four SNPs in the coding region of the CmGL gene were identified between the two parental lines; a single base substitution from C to A resulted in a premature termination codon and a truncated protein in the cmgl. The SNP was converted into a dCAPS marker, which showed co-segregation in the F2 population and 564 melon accessions. Result of this study will be helpful for better understanding of genetic control of trichome development in melon and marker-assisted selection in developing new cultivars.

Authors+Show Affiliations

College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China. Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou, 450002, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China. Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou, 450002, China.USDA-ARS Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, Madison, WI, 53706, USA.College of Horticulture, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China. ylmhort@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29147724

Citation

Zhu, Huayu, et al. "GLABROUS (CmGL) Encodes a HD-ZIP IV Transcription Factor Playing Roles in Multicellular Trichome Initiation in Melon." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 3, 2018, pp. 569-579.
Zhu H, Sun X, Zhang Q, et al. GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon. Theor Appl Genet. 2018;131(3):569-579.
Zhu, H., Sun, X., Zhang, Q., Song, P., Hu, Q., Zhang, X., Li, X., Hu, J., Pan, J., Sun, S., Weng, Y., & Yang, L. (2018). GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(3), 569-579. https://doi.org/10.1007/s00122-017-3019-9
Zhu H, et al. GLABROUS (CmGL) Encodes a HD-ZIP IV Transcription Factor Playing Roles in Multicellular Trichome Initiation in Melon. Theor Appl Genet. 2018;131(3):569-579. PubMed PMID: 29147724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon. AU - Zhu,Huayu, AU - Sun,Xiaofen, AU - Zhang,Qi, AU - Song,Pengyao, AU - Hu,Qianmei, AU - Zhang,Xiaojing, AU - Li,Xiang, AU - Hu,Jianbin, AU - Pan,Junsong, AU - Sun,Shouru, AU - Weng,Yiqun, AU - Yang,Luming, Y1 - 2017/11/17/ PY - 2017/09/05/received PY - 2017/11/10/accepted PY - 2017/11/18/pubmed PY - 2018/4/27/medline PY - 2017/11/18/entrez SP - 569 EP - 579 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 3 N2 - KEY MESSAGE: Map-based cloning identified CmGL that encodes a HD-ZIP type IV transcription factor that controls multicellular trichome initiation in melon. Trichomes are small hairs covering the aerial parts of plants that originate from the epidermal cells, which can protect plants against the damage by insects and pathogens. The regulatory pathway of unicellular trichomes has been well studied in the model plant Arabidopsis. Little is known about the genetic control and regulation of trichome development in melon (Cucumis melo L.) which has multicellular trichomes. In this study, we identified a melon mutant, cmgl, which showed completely glabrous on all aerial organs. A bulked segregant analysis was conducted to identify polymorphic markers for linkage analysis in a population with 256 F2 plants, which allowed to locate the cmgl locus in melon chromosome VIII. Next-generation sequencing-aided marker discovery and fine mapping in a large population with 1536 F2 plants narrowed the candidate gene region to 12 kb that harbored only one candidate gene for cmgl, which encoded a class IV homeodomain-associated leucine zipper transcription factor. Four SNPs in the coding region of the CmGL gene were identified between the two parental lines; a single base substitution from C to A resulted in a premature termination codon and a truncated protein in the cmgl. The SNP was converted into a dCAPS marker, which showed co-segregation in the F2 population and 564 melon accessions. Result of this study will be helpful for better understanding of genetic control of trichome development in melon and marker-assisted selection in developing new cultivars. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29147724/GLABROUS__CmGL__encodes_a_HD_ZIP_IV_transcription_factor_playing_roles_in_multicellular_trichome_initiation_in_melon_ L2 - https://dx.doi.org/10.1007/s00122-017-3019-9 DB - PRIME DP - Unbound Medicine ER -