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New Insights into Phasmatodea Chromosomes.
Genes (Basel) 2017; 8(11)G

Abstract

Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridization (FISH) was applied exclusively in two Leptynia species. Interestingly, 10-25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely. Chromosome preparation from embryos of five Phasmatodea species (Medauroidea extradentata, Sungaya inexpectata, Sipyloidea sipylus, Phaenopharos khaoyaiensis, and Peruphasma schultei) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e., S. inexpectata, P. khaoyaiensis, and P. schultei. Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea.

Authors+Show Affiliations

Institute of Human Genetics, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany. Thomas.Liehr@med.uni-jena.de.Novosibirsk State University, 630090 Novosibirsk, Russia. buleu.olesya@mail.ru.Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia. kary@bionet.nsc.ru.Novosibirsk State University, 630090 Novosibirsk, Russia. bugrov04@yahoo.co.uk. Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia. bugrov04@yahoo.co.uk.Novosibirsk State University, 630090 Novosibirsk, Russia. rubt@bionet.nsc.ru. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia. rubt@bionet.nsc.ru.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29149047

Citation

Liehr, Thomas, et al. "New Insights Into Phasmatodea Chromosomes." Genes, vol. 8, no. 11, 2017.
Liehr T, Buleu O, Karamysheva T, et al. New Insights into Phasmatodea Chromosomes. Genes (Basel). 2017;8(11).
Liehr, T., Buleu, O., Karamysheva, T., Bugrov, A., & Rubtsov, N. (2017). New Insights into Phasmatodea Chromosomes. Genes, 8(11), doi:10.3390/genes8110327.
Liehr T, et al. New Insights Into Phasmatodea Chromosomes. Genes (Basel). 2017 Nov 17;8(11) PubMed PMID: 29149047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New Insights into Phasmatodea Chromosomes. AU - Liehr,Thomas, AU - Buleu,Olesya, AU - Karamysheva,Tatyana, AU - Bugrov,Alexander, AU - Rubtsov,Nikolai, Y1 - 2017/11/17/ PY - 2017/09/19/received PY - 2017/11/13/revised PY - 2017/11/13/accepted PY - 2017/11/18/entrez PY - 2017/11/18/pubmed PY - 2017/11/18/medline KW - C-banding KW - Phasmatodea KW - fluorescence in situ hybridization KW - interstitial telomeric sequences KW - ribosomal deoxyribonucleic acid KW - stick insects KW - telomeric repeats JF - Genes JO - Genes (Basel) VL - 8 IS - 11 N2 - Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridization (FISH) was applied exclusively in two Leptynia species. Interestingly, 10-25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely. Chromosome preparation from embryos of five Phasmatodea species (Medauroidea extradentata, Sungaya inexpectata, Sipyloidea sipylus, Phaenopharos khaoyaiensis, and Peruphasma schultei) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e., S. inexpectata, P. khaoyaiensis, and P. schultei. Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea. SN - 2073-4425 UR - https://www.unboundmedicine.com/medline/citation/29149047/New_Insights_into_Phasmatodea_Chromosomes_ L2 - http://www.mdpi.com/resolver?pii=genes8110327 DB - PRIME DP - Unbound Medicine ER -