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Overexpression of Seipin1 Increases Oil in Hydroxy Fatty Acid-Accumulating Seeds.
Plant Cell Physiol. 2018 Jan 01; 59(1):205-214.PC

Abstract

While plant oils are an important source of food, plants also produce oils containing specialized fatty acids with chemical and physical properties valued in industry. Ricinoleic acid, a hydroxy fatty acid (HFA) produced in the seed of castor (Ricinus communis), is of particular value, with a wide range of applications. Since castor cultivation is currently successful only in tropical climates, and because castor seed contain the toxin ricin, there are ongoing efforts to develop a temperate crop capable of HFA biosynthesis. In castor, ricinoleic acid is incorporated into triacylglycerol (TAG) which accumulates in the seed lipid droplets. Research in the model plant Arabidopsis (Arabidopsis thaliana) has successfully produced HFA constituting 30% of the total seed oil, but this is far short of the level required to engineer commercially viable crops. Strategies to increase HFA have centered on co-expression of castor TAG biosynthesis enzymes. However, since lipid droplets are the location of neutral lipid storage, manipulating droplets offers an alternative method to increase oil that contains specialized fatty acids. The Arabidopsis Seipin1 protein modulates TAG accumulation by affecting lipid droplet size. Here, we overexpress Seipin1 in a hydroxylase-expressing Arabidopsis line, increasing seed HFA by 62% and proportionally increasing total oil. Increased seed oil was concomitant with a 22% increase in single seed weight and a 69% increase in harvest weight, while seed germination rose by 45%. Because Seipin1 function is unaffected by the structure of the HFA, these results demonstrate a novel strategy that may increase accumulation of many specialized seed oils.

Authors+Show Affiliations

Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29149288

Citation

Lunn, Daniel, et al. "Overexpression of Seipin1 Increases Oil in Hydroxy Fatty Acid-Accumulating Seeds." Plant & Cell Physiology, vol. 59, no. 1, 2018, pp. 205-214.
Lunn D, Wallis JG, Browse J. Overexpression of Seipin1 Increases Oil in Hydroxy Fatty Acid-Accumulating Seeds. Plant Cell Physiol. 2018;59(1):205-214.
Lunn, D., Wallis, J. G., & Browse, J. (2018). Overexpression of Seipin1 Increases Oil in Hydroxy Fatty Acid-Accumulating Seeds. Plant & Cell Physiology, 59(1), 205-214. https://doi.org/10.1093/pcp/pcx177
Lunn D, Wallis JG, Browse J. Overexpression of Seipin1 Increases Oil in Hydroxy Fatty Acid-Accumulating Seeds. Plant Cell Physiol. 2018 Jan 1;59(1):205-214. PubMed PMID: 29149288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of Seipin1 Increases Oil in Hydroxy Fatty Acid-Accumulating Seeds. AU - Lunn,Daniel, AU - Wallis,James G, AU - Browse,John, PY - 2017/10/02/received PY - 2017/11/10/accepted PY - 2017/11/18/pubmed PY - 2018/8/14/medline PY - 2017/11/18/entrez KW - Arabidopsis thaliana KW - Hydroxy-fatty acid KW - Lipid droplet KW - Lipid partitioning KW - Seipin1 KW - Triacylglycerol SP - 205 EP - 214 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 59 IS - 1 N2 - While plant oils are an important source of food, plants also produce oils containing specialized fatty acids with chemical and physical properties valued in industry. Ricinoleic acid, a hydroxy fatty acid (HFA) produced in the seed of castor (Ricinus communis), is of particular value, with a wide range of applications. Since castor cultivation is currently successful only in tropical climates, and because castor seed contain the toxin ricin, there are ongoing efforts to develop a temperate crop capable of HFA biosynthesis. In castor, ricinoleic acid is incorporated into triacylglycerol (TAG) which accumulates in the seed lipid droplets. Research in the model plant Arabidopsis (Arabidopsis thaliana) has successfully produced HFA constituting 30% of the total seed oil, but this is far short of the level required to engineer commercially viable crops. Strategies to increase HFA have centered on co-expression of castor TAG biosynthesis enzymes. However, since lipid droplets are the location of neutral lipid storage, manipulating droplets offers an alternative method to increase oil that contains specialized fatty acids. The Arabidopsis Seipin1 protein modulates TAG accumulation by affecting lipid droplet size. Here, we overexpress Seipin1 in a hydroxylase-expressing Arabidopsis line, increasing seed HFA by 62% and proportionally increasing total oil. Increased seed oil was concomitant with a 22% increase in single seed weight and a 69% increase in harvest weight, while seed germination rose by 45%. Because Seipin1 function is unaffected by the structure of the HFA, these results demonstrate a novel strategy that may increase accumulation of many specialized seed oils. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/29149288/Overexpression_of_Seipin1_Increases_Oil_in_Hydroxy_Fatty_Acid_Accumulating_Seeds_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcx177 DB - PRIME DP - Unbound Medicine ER -