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A novel allele of TaGW2-A1 is located in a finely mapped QTL that increases grain weight but decreases grain number in wheat (Triticum aestivum L.).
Theor Appl Genet. 2018 Mar; 131(3):539-553.TA

Abstract

KEY MESSAGE

A novel TaGW2-A1 allele was identified from a stable, robust QTL region, which is pleiotropic for thousand grain weight, grain number per spike, and grain morphometric parameters in wheat. Thousand grain weight (TGW) and grain number per spike (GNS) are two crucial determinants of wheat spike yield, and genetic dissection of their relationships can help to fine-tune these two components and maximize grain yield. By evaluating 191 recombinant inbred lines in 11 field trials, we identified five genomic regions on chromosomes 1B, 3A, 3B, 5B, or 7A that solely influenced either TGW or GNS, and a further region on chromosome 6A that concurrently affected TGW and GNS. The QTL of interest on chromosome 6A, which was flanked by wsnp_BE490604A_Ta_2_1 and wsnp_RFL_Contig1340_448996 and designated as QTgw/Gns.cau-6A, was finely mapped to a genetic interval shorter than 0.538 cM using near isogenic lines (NILs). The elite NILs of QTgw/Gns.cau-6A increased TGW by 8.33%, but decreased GNS by 3.05% in six field trials. Grain Weight 2 (TaGW2-A1), a well-characterized gene that negatively regulates TGW and grain width in wheat, was located within the finely mapped interval of QTgw/Gns.cau-6A. A novel and rare TaGW2-A1 allele with a 114-bp deletion in the 5' flanking region was identified in the parent with higher TGW, and it reduced TaGW2-A1 promoter activity and expression. In conclusion, these results expand our knowledge of the genetic and molecular basis of TGW-GNS trade-offs in wheat. The QTLs and the novel TaGW2-A1 allele are likely useful for the development of cultivars with higher TGW and/or higher GNS.

Authors+Show Affiliations

State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.Millet Research Institute, Shanxi Academy of Agricultural Sciences, Changzhi, 046011, Shanxi, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China. nizf@cau.edu.cn. National Plant Gene Research Centre, Beijing, 100193, China. nizf@cau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29150697

Citation

Zhai, Huijie, et al. "A Novel Allele of TaGW2-A1 Is Located in a Finely Mapped QTL That Increases Grain Weight but Decreases Grain Number in Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 3, 2018, pp. 539-553.
Zhai H, Feng Z, Du X, et al. A novel allele of TaGW2-A1 is located in a finely mapped QTL that increases grain weight but decreases grain number in wheat (Triticum aestivum L.). Theor Appl Genet. 2018;131(3):539-553.
Zhai, H., Feng, Z., Du, X., Song, Y., Liu, X., Qi, Z., Song, L., Li, J., Li, L., Peng, H., Hu, Z., Yao, Y., Xin, M., Xiao, S., Sun, Q., & Ni, Z. (2018). A novel allele of TaGW2-A1 is located in a finely mapped QTL that increases grain weight but decreases grain number in wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(3), 539-553. https://doi.org/10.1007/s00122-017-3017-y
Zhai H, et al. A Novel Allele of TaGW2-A1 Is Located in a Finely Mapped QTL That Increases Grain Weight but Decreases Grain Number in Wheat (Triticum Aestivum L.). Theor Appl Genet. 2018;131(3):539-553. PubMed PMID: 29150697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel allele of TaGW2-A1 is located in a finely mapped QTL that increases grain weight but decreases grain number in wheat (Triticum aestivum L.). AU - Zhai,Huijie, AU - Feng,Zhiyu, AU - Du,Xiaofen, AU - Song,Yane, AU - Liu,Xinye, AU - Qi,Zhongqi, AU - Song,Long, AU - Li,Jiang, AU - Li,Linghong, AU - Peng,Huiru, AU - Hu,Zhaorong, AU - Yao,Yingyin, AU - Xin,Mingming, AU - Xiao,Shihe, AU - Sun,Qixin, AU - Ni,Zhongfu, Y1 - 2017/11/17/ PY - 2017/05/16/received PY - 2017/11/04/accepted PY - 2017/11/19/pubmed PY - 2018/4/27/medline PY - 2017/11/19/entrez SP - 539 EP - 553 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 3 N2 - KEY MESSAGE: A novel TaGW2-A1 allele was identified from a stable, robust QTL region, which is pleiotropic for thousand grain weight, grain number per spike, and grain morphometric parameters in wheat. Thousand grain weight (TGW) and grain number per spike (GNS) are two crucial determinants of wheat spike yield, and genetic dissection of their relationships can help to fine-tune these two components and maximize grain yield. By evaluating 191 recombinant inbred lines in 11 field trials, we identified five genomic regions on chromosomes 1B, 3A, 3B, 5B, or 7A that solely influenced either TGW or GNS, and a further region on chromosome 6A that concurrently affected TGW and GNS. The QTL of interest on chromosome 6A, which was flanked by wsnp_BE490604A_Ta_2_1 and wsnp_RFL_Contig1340_448996 and designated as QTgw/Gns.cau-6A, was finely mapped to a genetic interval shorter than 0.538 cM using near isogenic lines (NILs). The elite NILs of QTgw/Gns.cau-6A increased TGW by 8.33%, but decreased GNS by 3.05% in six field trials. Grain Weight 2 (TaGW2-A1), a well-characterized gene that negatively regulates TGW and grain width in wheat, was located within the finely mapped interval of QTgw/Gns.cau-6A. A novel and rare TaGW2-A1 allele with a 114-bp deletion in the 5' flanking region was identified in the parent with higher TGW, and it reduced TaGW2-A1 promoter activity and expression. In conclusion, these results expand our knowledge of the genetic and molecular basis of TGW-GNS trade-offs in wheat. The QTLs and the novel TaGW2-A1 allele are likely useful for the development of cultivars with higher TGW and/or higher GNS. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29150697/A_novel_allele_of_TaGW2_A1_is_located_in_a_finely_mapped_QTL_that_increases_grain_weight_but_decreases_grain_number_in_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-017-3017-y DB - PRIME DP - Unbound Medicine ER -