Tags

Type your tag names separated by a space and hit enter

Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation.
Appl Microbiol Biotechnol 2018; 102(2):921-932AM

Abstract

Malolactic fermentation (MLF) is an important step in winemaking, which can be notoriously unreliable due to the fastidious nature of Oenococcus oeni. This study aimed to use directed evolution (DE) to produce a more robust strain of O. oeni having the ability to withstand high ethanol concentrations. DE involves an organism mutating and potentially adapting to a high stress environment over the course of extended cultivation. A continuous culture of O. oeni was established and exposed to progressively increasing ethanol content such that after approximately 330 generations, an isolate from this culture was able to complete MLF in high ethanol content medium earlier than its parent. The ethanol tolerance of a single isolate, A90, was tested to confirm the phenotype and its fermentation performance in wine. In order to investigate the genotypic differences in the evolved strain that led to the ethanol tolerance phenotype, the relative expression of a number of known stress response genes was compared between SB3 and A90. Notably, there was increase in hsp18 expression in 20% (v/v) ethanol by both strains with A90 exhibiting a higher degree of expression. This study is the first to use directed evolution for O. oeni strain improvement and confirms that this technique can be used successfully for the development of new candidate strains for the wine industry. This study also adds to the current knowledge on the genetic basis of ethanol tolerance in this bacterium.

Authors+Show Affiliations

Department of Wine and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia.Department of Wine and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia.Department of Wine and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia.Department of Wine and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia.Department of Wine and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia. vladimir.jiranek@adelaide.edu.au. Australian Research Council Training Centre for Innovative Wine Production, Urrbrae, South Australia, Australia. vladimir.jiranek@adelaide.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29150706

Citation

Betteridge, Alice L., et al. "Application of Directed Evolution to Develop Ethanol Tolerant Oenococcus Oeni for More Efficient Malolactic Fermentation." Applied Microbiology and Biotechnology, vol. 102, no. 2, 2018, pp. 921-932.
Betteridge AL, Sumby KM, Sundstrom JF, et al. Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation. Appl Microbiol Biotechnol. 2018;102(2):921-932.
Betteridge, A. L., Sumby, K. M., Sundstrom, J. F., Grbin, P. R., & Jiranek, V. (2018). Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation. Applied Microbiology and Biotechnology, 102(2), pp. 921-932. doi:10.1007/s00253-017-8593-x.
Betteridge AL, et al. Application of Directed Evolution to Develop Ethanol Tolerant Oenococcus Oeni for More Efficient Malolactic Fermentation. Appl Microbiol Biotechnol. 2018;102(2):921-932. PubMed PMID: 29150706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation. AU - Betteridge,Alice L, AU - Sumby,Krista M, AU - Sundstrom,Joanna F, AU - Grbin,Paul R, AU - Jiranek,Vladimir, Y1 - 2017/11/17/ PY - 2017/04/12/received PY - 2017/10/12/accepted PY - 2017/10/09/revised PY - 2017/11/19/pubmed PY - 2019/2/13/medline PY - 2017/11/19/entrez KW - Directed evolution KW - Ethanol KW - Malolactic fermentation KW - Oenococcus KW - Stress response KW - Wine SP - 921 EP - 932 JF - Applied microbiology and biotechnology JO - Appl. Microbiol. Biotechnol. VL - 102 IS - 2 N2 - Malolactic fermentation (MLF) is an important step in winemaking, which can be notoriously unreliable due to the fastidious nature of Oenococcus oeni. This study aimed to use directed evolution (DE) to produce a more robust strain of O. oeni having the ability to withstand high ethanol concentrations. DE involves an organism mutating and potentially adapting to a high stress environment over the course of extended cultivation. A continuous culture of O. oeni was established and exposed to progressively increasing ethanol content such that after approximately 330 generations, an isolate from this culture was able to complete MLF in high ethanol content medium earlier than its parent. The ethanol tolerance of a single isolate, A90, was tested to confirm the phenotype and its fermentation performance in wine. In order to investigate the genotypic differences in the evolved strain that led to the ethanol tolerance phenotype, the relative expression of a number of known stress response genes was compared between SB3 and A90. Notably, there was increase in hsp18 expression in 20% (v/v) ethanol by both strains with A90 exhibiting a higher degree of expression. This study is the first to use directed evolution for O. oeni strain improvement and confirms that this technique can be used successfully for the development of new candidate strains for the wine industry. This study also adds to the current knowledge on the genetic basis of ethanol tolerance in this bacterium. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/29150706/Application_of_directed_evolution_to_develop_ethanol_tolerant_Oenococcus_oeni_for_more_efficient_malolactic_fermentation_ L2 - https://dx.doi.org/10.1007/s00253-017-8593-x DB - PRIME DP - Unbound Medicine ER -