Tags

Type your tag names separated by a space and hit enter

Seasonal analysis and acaricidal activity of the thymol-type essential oil of Ocimum gratissimum and its major constituents against Rhipicephalus microplus (Acari: Ixodidae).
Parasitol Res. 2018 Jan; 117(1):59-65.PR

Abstract

The tick Rhipicephalus microplus affects cattle health, with production loss in tropical and subtropical regions. Moreover, the use of commercial acaricides has been reduced due to the resistance of this parasite. Although alternatives such as plant bioactive molecules have been sought, essential oils present variations in their chemical constituents due to environmental factors, which can interfere with their acaricidal activity. The objective of the present study was to evaluate the seasonal influence of the essential oil of Ocimum gratissimum and its major constituents on acaricidal activity against R. microplus larvae. A high-yield essential oil of O. gratissimum and its major constituents were used, and a plant with a thymol-type oil was selected for seasonal analysis and acaricidal activity against R. microplus. Gas chromatography (GC) and GC-mass spectrometry (MS) were employed to identify 31 oil constituents (average yield of 6.26%). The main compounds were found to be thymol (33.4 to 47.9%), γ-terpinene (26.2 to 36.8%), and p-cymene (4.3 to 17.0%). Concerning acaricidal activity, the December (LC50 0.84 mg/mL) and September (LC50 1.58 mg/mL) oils obtained in the dry season were the most active, and assays performed with commercial standards revealed LC50 values of p-cymene, thymol, and γ-terpinene of 1.41, 1.81, and 3.08 mg/mL, respectively. Overall, lower acaricidal activities were found for oils produced from plants harvested in the rainy season. The results showed that seasonal variation in the chemical composition of the O. gratissimum essential oil influences its acaricidal activity. The seasonal variations in the thymol-type essential oil of O. gratissimum can represent an important strategy for the control of R. microplus.

Authors+Show Affiliations

Departamento de Patologia, Universidade Federal do Maranhão, São Luís, MA, Brazil.Programa de Pós Graduação em Ciências da Saúde, Universidade Federal do Maranhão, São Luís, MA, Brazil.Programa de Pós-Graduação em Química, Universidade Federal do Maranhão, São Luís, MA, Brazil.Departamento de Patologia, Universidade Federal do Maranhão, São Luís, MA, Brazil.Programa de Pós-Graduação em Química, Universidade Federal do Maranhão, São Luís, MA, Brazil.Programa de Pós-Graduação em Química, Universidade Federal do Maranhão, São Luís, MA, Brazil.Programa de Pós-Graduação em Recursos Naturais da Amazônia, Universidade Federal do Oeste do Pará, Santarém, PA, Brazil.Departamento de Patologia, Universidade Federal do Maranhão, São Luís, MA, Brazil. livio.martins@ufma.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29152690

Citation

Silva Lima, Aldilene, et al. "Seasonal Analysis and Acaricidal Activity of the Thymol-type Essential Oil of Ocimum Gratissimum and Its Major Constituents Against Rhipicephalus Microplus (Acari: Ixodidae)." Parasitology Research, vol. 117, no. 1, 2018, pp. 59-65.
Silva Lima A, Milhomem MN, Santos Monteiro O, et al. Seasonal analysis and acaricidal activity of the thymol-type essential oil of Ocimum gratissimum and its major constituents against Rhipicephalus microplus (Acari: Ixodidae). Parasitol Res. 2018;117(1):59-65.
Silva Lima, A., Milhomem, M. N., Santos Monteiro, O., Arruda, A. C. P., de Castro, J. A. M., Fernandes, Y. M. L., Maia, J. G. S., & Costa-Junior, L. M. (2018). Seasonal analysis and acaricidal activity of the thymol-type essential oil of Ocimum gratissimum and its major constituents against Rhipicephalus microplus (Acari: Ixodidae). Parasitology Research, 117(1), 59-65. https://doi.org/10.1007/s00436-017-5662-0
Silva Lima A, et al. Seasonal Analysis and Acaricidal Activity of the Thymol-type Essential Oil of Ocimum Gratissimum and Its Major Constituents Against Rhipicephalus Microplus (Acari: Ixodidae). Parasitol Res. 2018;117(1):59-65. PubMed PMID: 29152690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Seasonal analysis and acaricidal activity of the thymol-type essential oil of Ocimum gratissimum and its major constituents against Rhipicephalus microplus (Acari: Ixodidae). AU - Silva Lima,Aldilene, AU - Milhomem,Marlise Neves, AU - Santos Monteiro,Odair, AU - Arruda,Alana Carynne Pereira, AU - de Castro,Joaquim Antônio Martins, AU - Fernandes,Yan Michel Lopes, AU - Maia,José Guilherme Soares, AU - Costa-Junior,Lívio Martins, Y1 - 2017/11/19/ PY - 2017/01/23/received PY - 2017/10/19/accepted PY - 2017/11/21/pubmed PY - 2018/4/26/medline PY - 2017/11/21/entrez KW - Acaricidal activity KW - Natural product KW - Thymol KW - Tick KW - p-Cymene KW - γ-Terpinene SP - 59 EP - 65 JF - Parasitology research JO - Parasitol. Res. VL - 117 IS - 1 N2 - The tick Rhipicephalus microplus affects cattle health, with production loss in tropical and subtropical regions. Moreover, the use of commercial acaricides has been reduced due to the resistance of this parasite. Although alternatives such as plant bioactive molecules have been sought, essential oils present variations in their chemical constituents due to environmental factors, which can interfere with their acaricidal activity. The objective of the present study was to evaluate the seasonal influence of the essential oil of Ocimum gratissimum and its major constituents on acaricidal activity against R. microplus larvae. A high-yield essential oil of O. gratissimum and its major constituents were used, and a plant with a thymol-type oil was selected for seasonal analysis and acaricidal activity against R. microplus. Gas chromatography (GC) and GC-mass spectrometry (MS) were employed to identify 31 oil constituents (average yield of 6.26%). The main compounds were found to be thymol (33.4 to 47.9%), γ-terpinene (26.2 to 36.8%), and p-cymene (4.3 to 17.0%). Concerning acaricidal activity, the December (LC50 0.84 mg/mL) and September (LC50 1.58 mg/mL) oils obtained in the dry season were the most active, and assays performed with commercial standards revealed LC50 values of p-cymene, thymol, and γ-terpinene of 1.41, 1.81, and 3.08 mg/mL, respectively. Overall, lower acaricidal activities were found for oils produced from plants harvested in the rainy season. The results showed that seasonal variation in the chemical composition of the O. gratissimum essential oil influences its acaricidal activity. The seasonal variations in the thymol-type essential oil of O. gratissimum can represent an important strategy for the control of R. microplus. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/29152690/Seasonal_analysis_and_acaricidal_activity_of_the_thymol_type_essential_oil_of_Ocimum_gratissimum_and_its_major_constituents_against_Rhipicephalus_microplus__Acari:_Ixodidae__ L2 - https://dx.doi.org/10.1007/s00436-017-5662-0 DB - PRIME DP - Unbound Medicine ER -