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MicroRNA-30a-3p is overexpressed in the placentas of patients with preeclampsia and affects trophoblast invasion and apoptosis by its effects on IGF-1.
Am J Obstet Gynecol 2018; 218(2):249.e1-249.e12AJ

Abstract

OBJECTIVE

Preeclampsia (PE) affects many women globally and remains a primary cause of neonatal and maternal morbidity and mortality. Aberrant placental microRNA (miRNA) expression might be associated with PE. Previously, 33 PE-related miRNAs, 11 up-regulated and 23 down-regulated, were detected in placentas of women with severe PE when compared with those of normal patients. One of the most up-regulated miRNAs in PE is miR-30a-3p. The predicted target of it is insulin-like growth factor 1 (IGF-1), which has been reported to have a relatively low expression level in PE patients. This study was conducted to determine the aberrant increased of miR-30a-3p in the placentas of women with preeclampsia and to elucidate the target and function of it in trophoblast cells.

STUDY DESIGN

miR-30a-3p expression in placenta tissues was compared between women with preeclampsia (n = 25) and normal pregnant women (n = 20). The miRNA target was studied by in silico and functional assay. The effects of the miRNA were verified by apoptosis assay and invasion assay in the trophoblast cell line.

RESULTS

miR-30a-3p was increased significantly in the placenta of women with preeclampsia when compared to those with normal pregnancies. Luciferase assay confirmed direct regulation of miR-30a-3p on the expression of IGF-1. Forced expression of miR-30a-3p suppressed IGF-1 protein expression in the HTR-8/SVneo cells. The functional assay suggests that the over-expression of miR-30a-3p alter the invasive capacity of JEG-3 cells and induce the apoptosis of HTR-8/SVneo cells (Figure).

CONCLUSION

Expression of miR-30a-3p was significantly increased in the placentas of patients with preeclampsia. miR-30a-3p might be involved in the pathogenesis of preeclampsia by targeting IGF-1 and regulating the invasion and apoptosis of trophoblast cells.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China; Department of Obstetrics and Gynecology, The University of Hong Kong, Hong Kong, China.Department of Orthopedics, Hainan Branch of PLA General Hospital, Sanya, China.Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China. Electronic address: xiaomingzhu1981@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29155142

Citation

Niu, Zi-Ru, et al. "MicroRNA-30a-3p Is Overexpressed in the Placentas of Patients With Preeclampsia and Affects Trophoblast Invasion and Apoptosis By Its Effects On IGF-1." American Journal of Obstetrics and Gynecology, vol. 218, no. 2, 2018, pp. 249.e1-249.e12.
Niu ZR, Han T, Sun XL, et al. MicroRNA-30a-3p is overexpressed in the placentas of patients with preeclampsia and affects trophoblast invasion and apoptosis by its effects on IGF-1. Am J Obstet Gynecol. 2018;218(2):249.e1-249.e12.
Niu, Z. R., Han, T., Sun, X. L., Luan, L. X., Gou, W. L., & Zhu, X. M. (2018). MicroRNA-30a-3p is overexpressed in the placentas of patients with preeclampsia and affects trophoblast invasion and apoptosis by its effects on IGF-1. American Journal of Obstetrics and Gynecology, 218(2), pp. 249.e1-249.e12. doi:10.1016/j.ajog.2017.11.568.
Niu ZR, et al. MicroRNA-30a-3p Is Overexpressed in the Placentas of Patients With Preeclampsia and Affects Trophoblast Invasion and Apoptosis By Its Effects On IGF-1. Am J Obstet Gynecol. 2018;218(2):249.e1-249.e12. PubMed PMID: 29155142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA-30a-3p is overexpressed in the placentas of patients with preeclampsia and affects trophoblast invasion and apoptosis by its effects on IGF-1. AU - Niu,Zi-Ru, AU - Han,Tao, AU - Sun,Xiao-Luan, AU - Luan,Li-Xia, AU - Gou,Wen-Li, AU - Zhu,Xiao-Ming, Y1 - 2017/11/16/ PY - 2017/10/27/received PY - 2017/10/27/revised PY - 2017/11/08/accepted PY - 2017/11/21/pubmed PY - 2018/7/17/medline PY - 2017/11/21/entrez KW - IGF-1 KW - apoptosis KW - epigenetics KW - functional assays KW - invasion KW - microRNA KW - placenta KW - preeclampsia KW - trophoblast SP - 249.e1 EP - 249.e12 JF - American journal of obstetrics and gynecology JO - Am. J. Obstet. Gynecol. VL - 218 IS - 2 N2 - OBJECTIVE: Preeclampsia (PE) affects many women globally and remains a primary cause of neonatal and maternal morbidity and mortality. Aberrant placental microRNA (miRNA) expression might be associated with PE. Previously, 33 PE-related miRNAs, 11 up-regulated and 23 down-regulated, were detected in placentas of women with severe PE when compared with those of normal patients. One of the most up-regulated miRNAs in PE is miR-30a-3p. The predicted target of it is insulin-like growth factor 1 (IGF-1), which has been reported to have a relatively low expression level in PE patients. This study was conducted to determine the aberrant increased of miR-30a-3p in the placentas of women with preeclampsia and to elucidate the target and function of it in trophoblast cells. STUDY DESIGN: miR-30a-3p expression in placenta tissues was compared between women with preeclampsia (n = 25) and normal pregnant women (n = 20). The miRNA target was studied by in silico and functional assay. The effects of the miRNA were verified by apoptosis assay and invasion assay in the trophoblast cell line. RESULTS: miR-30a-3p was increased significantly in the placenta of women with preeclampsia when compared to those with normal pregnancies. Luciferase assay confirmed direct regulation of miR-30a-3p on the expression of IGF-1. Forced expression of miR-30a-3p suppressed IGF-1 protein expression in the HTR-8/SVneo cells. The functional assay suggests that the over-expression of miR-30a-3p alter the invasive capacity of JEG-3 cells and induce the apoptosis of HTR-8/SVneo cells (Figure). CONCLUSION: Expression of miR-30a-3p was significantly increased in the placentas of patients with preeclampsia. miR-30a-3p might be involved in the pathogenesis of preeclampsia by targeting IGF-1 and regulating the invasion and apoptosis of trophoblast cells. SN - 1097-6868 UR - https://www.unboundmedicine.com/medline/citation/29155142/MicroRNA_30a_3p_is_overexpressed_in_the_placentas_of_patients_with_preeclampsia_and_affects_trophoblast_invasion_and_apoptosis_by_its_effects_on_IGF_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9378(17)32333-5 DB - PRIME DP - Unbound Medicine ER -