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Matrine inhibiting pancreatic cells epithelial-mesenchymal transition and invasion through ROS/NF-κB/MMPs pathway.
Life Sci. 2018 Jan 01; 192:55-61.LS

Abstract

AIMS

Matrine has demonstrated an exclusive anti-tumor effect, including inhibiting cancer cells proliferation and inducing cancer cells apoptosis and autophagy. Whether it can inhibit cancer cells invasion is remain obscure.

MAIN METHODS

The Panc-1 cells were cultured with matrine, NAC and methanol, wound healing assay and transwell invasion assay were applied to detect the migration and invasion ability. The expression of MMP-2 and MMP-9 were assessed, as well as the Epithelial-Mesenchymal Transition marker. Further detect the expression of pP65, total P65, pIκBα, total IκBα, MMP-2, MMP-9 and Panc-1 cells migration and invasion ability to detect whether NF-κB signaling pathway is involved.

KEY FINDINGS

In matrine treated group, the expression of E-cadherin was up-regulated while N-cadherin, Vimentin was down-regulated. In addition, wound healing assay and transwell invasion assay showed that the cells treated with matrine expressed weaker migration and invasion ability, and MMP-2 and MMP-9 was down-regulated in matrine treated group. Further research reveals that the effect of Matrine could decreased the level of intracellular ROS. Furthermore, pP65, pIκBα level was down-regulated in the matrine and NAC group when compared to control group. The panc-1 cells showed less migration and invasion ability, as well as lower MMP-2 and MMP-9 expression in the group treated with NF-κBI along with H2O2 when compared with treated with H2O2 only.

SIGNIFICANCE

Matrine inhibit pancreatic cancer cells migration and invasion through ROS/NF-κB/MMPs pathway, further validate the anticancer effect of matrine.

Authors+Show Affiliations

Oncology Department, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China. Electronic address: hmntjzyy@126.com.Oncology Department, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29155301

Citation

Huang, Minna, and Wen Xin. "Matrine Inhibiting Pancreatic Cells Epithelial-mesenchymal Transition and Invasion Through ROS/NF-κB/MMPs Pathway." Life Sciences, vol. 192, 2018, pp. 55-61.
Huang M, Xin W. Matrine inhibiting pancreatic cells epithelial-mesenchymal transition and invasion through ROS/NF-κB/MMPs pathway. Life Sci. 2018;192:55-61.
Huang, M., & Xin, W. (2018). Matrine inhibiting pancreatic cells epithelial-mesenchymal transition and invasion through ROS/NF-κB/MMPs pathway. Life Sciences, 192, 55-61. https://doi.org/10.1016/j.lfs.2017.11.024
Huang M, Xin W. Matrine Inhibiting Pancreatic Cells Epithelial-mesenchymal Transition and Invasion Through ROS/NF-κB/MMPs Pathway. Life Sci. 2018 Jan 1;192:55-61. PubMed PMID: 29155301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrine inhibiting pancreatic cells epithelial-mesenchymal transition and invasion through ROS/NF-κB/MMPs pathway. AU - Huang,Minna, AU - Xin,Wen, Y1 - 2017/11/16/ PY - 2017/08/22/received PY - 2017/11/13/revised PY - 2017/11/15/accepted PY - 2017/11/21/pubmed PY - 2018/1/9/medline PY - 2017/11/21/entrez KW - EMT KW - Invasion KW - Matrine KW - NF-κB KW - Pancreatic cancer KW - ROS SP - 55 EP - 61 JF - Life sciences JO - Life Sci VL - 192 N2 - AIMS: Matrine has demonstrated an exclusive anti-tumor effect, including inhibiting cancer cells proliferation and inducing cancer cells apoptosis and autophagy. Whether it can inhibit cancer cells invasion is remain obscure. MAIN METHODS: The Panc-1 cells were cultured with matrine, NAC and methanol, wound healing assay and transwell invasion assay were applied to detect the migration and invasion ability. The expression of MMP-2 and MMP-9 were assessed, as well as the Epithelial-Mesenchymal Transition marker. Further detect the expression of pP65, total P65, pIκBα, total IκBα, MMP-2, MMP-9 and Panc-1 cells migration and invasion ability to detect whether NF-κB signaling pathway is involved. KEY FINDINGS: In matrine treated group, the expression of E-cadherin was up-regulated while N-cadherin, Vimentin was down-regulated. In addition, wound healing assay and transwell invasion assay showed that the cells treated with matrine expressed weaker migration and invasion ability, and MMP-2 and MMP-9 was down-regulated in matrine treated group. Further research reveals that the effect of Matrine could decreased the level of intracellular ROS. Furthermore, pP65, pIκBα level was down-regulated in the matrine and NAC group when compared to control group. The panc-1 cells showed less migration and invasion ability, as well as lower MMP-2 and MMP-9 expression in the group treated with NF-κBI along with H2O2 when compared with treated with H2O2 only. SIGNIFICANCE: Matrine inhibit pancreatic cancer cells migration and invasion through ROS/NF-κB/MMPs pathway, further validate the anticancer effect of matrine. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/29155301/Matrine_inhibiting_pancreatic_cells_epithelial_mesenchymal_transition_and_invasion_through_ROS/NF_κB/MMPs_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(17)30601-X DB - PRIME DP - Unbound Medicine ER -