Tags

Type your tag names separated by a space and hit enter

Label-free electrochemical DNA biosensor for guanine and adenine by ds-DNA/poly(L-cysteine)/Fe3O4 nanoparticles-graphene oxide nanocomposite modified electrode.
Biosens Bioelectron. 2018 Apr 15; 102:70-79.BB

Abstract

In this study, we aim to design a simple and effective electrochemical DNA biosensor based on a carbon paste electrode modified with ds-DNA/poly(L-cysteine)/Fe3O4 nanoparticles-graphene oxide (ds-DNA/p(L-Cys)/Fe3O4 NPs-GO/CPE) for sensitive detection of adenine (A) and guanine (G). The electrocatalytic oxidation of A and G on the electrode was explored by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). This sensor shows separated and well-defined peaks for A and G, by which one can determine these biological bases individually or simultaneously. The ds-DNA/p(L-Cys)/Fe3O4 NPs-GO/CPE exhibited an increase in peak currents and the electron transfer kinetics and decrease in the overpotential for the oxidation reaction of A and G. Under the optimal conditions a linear relationship is figured out between the peak current and the analytes' concentrations on a range of 0.01-30.0μM and 0.01-25.0μM for simultaneous determination of A and G, with detection limits of 3.48 and 1.59nM, respectively. As well as, individually determination is resulted two linear concentration ranges of 0.01-30.0μM for A and 0.01-25.0μM for G with detection limits of 3.90 and 1.58nM for A and G, respectively. The proposed biosensor exhibited some advantages in terms of simplicity, rapidity, high sensitivity, good reproducibility and long-term stability. Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G + C)/(A + T) of DNA was calculated as about 0.77 for various DNA samples. This study also ascertained that the proposed biosensor can be profitable to evaluate DNA bases damage.

Authors+Show Affiliations

Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914-41335, Rasht, Iran. Electronic address: arvand@guilan.ac.ir.Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914-41335, Rasht, Iran.Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914-41335, Rasht, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29156408

Citation

Arvand, Majid, et al. "Label-free Electrochemical DNA Biosensor for Guanine and Adenine By ds-DNA/poly(L-cysteine)/Fe3O4 Nanoparticles-graphene Oxide Nanocomposite Modified Electrode." Biosensors & Bioelectronics, vol. 102, 2018, pp. 70-79.
Arvand M, Sanayeei M, Hemmati S. Label-free electrochemical DNA biosensor for guanine and adenine by ds-DNA/poly(L-cysteine)/Fe3O4 nanoparticles-graphene oxide nanocomposite modified electrode. Biosens Bioelectron. 2018;102:70-79.
Arvand, M., Sanayeei, M., & Hemmati, S. (2018). Label-free electrochemical DNA biosensor for guanine and adenine by ds-DNA/poly(L-cysteine)/Fe3O4 nanoparticles-graphene oxide nanocomposite modified electrode. Biosensors & Bioelectronics, 102, 70-79. https://doi.org/10.1016/j.bios.2017.11.002
Arvand M, Sanayeei M, Hemmati S. Label-free Electrochemical DNA Biosensor for Guanine and Adenine By ds-DNA/poly(L-cysteine)/Fe3O4 Nanoparticles-graphene Oxide Nanocomposite Modified Electrode. Biosens Bioelectron. 2018 Apr 15;102:70-79. PubMed PMID: 29156408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free electrochemical DNA biosensor for guanine and adenine by ds-DNA/poly(L-cysteine)/Fe3O4 nanoparticles-graphene oxide nanocomposite modified electrode. AU - Arvand,Majid, AU - Sanayeei,Mona, AU - Hemmati,Shiva, Y1 - 2017/12/11/ PY - 2017/09/08/received PY - 2017/10/30/revised PY - 2017/11/01/accepted PY - 2017/11/21/pubmed PY - 2018/8/16/medline PY - 2017/11/21/entrez KW - Adenine KW - DNA biosensor KW - Electroanalytical technique KW - Guanine KW - Nanomaterials SP - 70 EP - 79 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 102 N2 - In this study, we aim to design a simple and effective electrochemical DNA biosensor based on a carbon paste electrode modified with ds-DNA/poly(L-cysteine)/Fe3O4 nanoparticles-graphene oxide (ds-DNA/p(L-Cys)/Fe3O4 NPs-GO/CPE) for sensitive detection of adenine (A) and guanine (G). The electrocatalytic oxidation of A and G on the electrode was explored by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). This sensor shows separated and well-defined peaks for A and G, by which one can determine these biological bases individually or simultaneously. The ds-DNA/p(L-Cys)/Fe3O4 NPs-GO/CPE exhibited an increase in peak currents and the electron transfer kinetics and decrease in the overpotential for the oxidation reaction of A and G. Under the optimal conditions a linear relationship is figured out between the peak current and the analytes' concentrations on a range of 0.01-30.0μM and 0.01-25.0μM for simultaneous determination of A and G, with detection limits of 3.48 and 1.59nM, respectively. As well as, individually determination is resulted two linear concentration ranges of 0.01-30.0μM for A and 0.01-25.0μM for G with detection limits of 3.90 and 1.58nM for A and G, respectively. The proposed biosensor exhibited some advantages in terms of simplicity, rapidity, high sensitivity, good reproducibility and long-term stability. Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G + C)/(A + T) of DNA was calculated as about 0.77 for various DNA samples. This study also ascertained that the proposed biosensor can be profitable to evaluate DNA bases damage. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/29156408/Label_free_electrochemical_DNA_biosensor_for_guanine_and_adenine_by_ds_DNA/poly_L_cysteine_/Fe3O4_nanoparticles_graphene_oxide_nanocomposite_modified_electrode_ DB - PRIME DP - Unbound Medicine ER -