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Switchable electrochemiluminescence aptasensor coupled with resonance energy transfer for selective attomolar detection of Hg2+ via CdTe@CdS/dendrimer probe and Au nanoparticle quencher.
Biosens Bioelectron. 2018 Apr 15; 102:328-335.BB

Abstract

In the present study, an ultrasensitive electrochemiluminescence (ECL) aptasensing assay for selective detection of Hg2+ was designed. In this electrochemiluminescence resonance energy transfer (ECL-RET) approach, Fe3O4@SiO2/dendrimers/QDs exhibited amplified ECL emissions (switch "on" state) and with the hybridization between T-rich ssDNA(S1) immobilized on the Fe3O4@SiO2/dendrimers/QDs and AuNPs modified with complementary aptamer (AuNPs-S2), the ECL of QDs nanocomposites was efficiently quenched (switch "off" state). In the presence of Hg2+ ions, formation of strong and stable T-Hg2+-T complex led to the release of the AuNPs-S2 from double-stranded DNA(dsDNA) and the recovery of the ECL signal of QDs (second signal switch "on" state). Under optimal conditions, Hg2+ can be detected in a wide linear range from 20aM to 2µM with a very low detection limit of 2aM. The proposed ECL aptasensor showed high selectivity for Hg2+ determination compared to other environmentally relevant metal ions at concentration ratio more than 1000 times. The aptasensor was used for detection Hg2+ ions from samples of tap waters, carp and saltwater fishes with satisfactory results. The aptasensor exhibited high sensitivity, wide linear response (11 orders of magnitude), excellent reproducibility and stability. The proposed aptasensor will be a promising candidate for facile and rapid determination of Hg2+in environmental and fishery samples.

Authors+Show Affiliations

Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran.Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; Research Center for Nanotechnology, University of Kurdistan, 66177-15175 Sanandaj, Iran. Electronic address: absalimi@yahoo.com.Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; Research Center for Nanotechnology, University of Kurdistan, 66177-15175 Sanandaj, Iran. Electronic address: rhallaj@uok.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29161665

Citation

Babamiri, Bahareh, et al. "Switchable Electrochemiluminescence Aptasensor Coupled With Resonance Energy Transfer for Selective Attomolar Detection of Hg2+ Via CdTe@CdS/dendrimer Probe and Au Nanoparticle Quencher." Biosensors & Bioelectronics, vol. 102, 2018, pp. 328-335.
Babamiri B, Salimi A, Hallaj R. Switchable electrochemiluminescence aptasensor coupled with resonance energy transfer for selective attomolar detection of Hg2+ via CdTe@CdS/dendrimer probe and Au nanoparticle quencher. Biosens Bioelectron. 2018;102:328-335.
Babamiri, B., Salimi, A., & Hallaj, R. (2018). Switchable electrochemiluminescence aptasensor coupled with resonance energy transfer for selective attomolar detection of Hg2+ via CdTe@CdS/dendrimer probe and Au nanoparticle quencher. Biosensors & Bioelectronics, 102, 328-335. https://doi.org/10.1016/j.bios.2017.11.034
Babamiri B, Salimi A, Hallaj R. Switchable Electrochemiluminescence Aptasensor Coupled With Resonance Energy Transfer for Selective Attomolar Detection of Hg2+ Via CdTe@CdS/dendrimer Probe and Au Nanoparticle Quencher. Biosens Bioelectron. 2018 Apr 15;102:328-335. PubMed PMID: 29161665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Switchable electrochemiluminescence aptasensor coupled with resonance energy transfer for selective attomolar detection of Hg2+ via CdTe@CdS/dendrimer probe and Au nanoparticle quencher. AU - Babamiri,Bahareh, AU - Salimi,Abdollah, AU - Hallaj,Rahman, Y1 - 2017/11/10/ PY - 2017/08/22/received PY - 2017/10/13/revised PY - 2017/11/07/accepted PY - 2017/11/22/pubmed PY - 2018/8/4/medline PY - 2017/11/22/entrez KW - CdTe@CdS quantum dots KW - Dendrimer KW - Electrochemiluminescence aptasensor KW - Fe(3)O(4)@SiO(2) nanoparticles KW - Hg(2+) KW - Resonance energy transfer (RET) SP - 328 EP - 335 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 102 N2 - In the present study, an ultrasensitive electrochemiluminescence (ECL) aptasensing assay for selective detection of Hg2+ was designed. In this electrochemiluminescence resonance energy transfer (ECL-RET) approach, Fe3O4@SiO2/dendrimers/QDs exhibited amplified ECL emissions (switch "on" state) and with the hybridization between T-rich ssDNA(S1) immobilized on the Fe3O4@SiO2/dendrimers/QDs and AuNPs modified with complementary aptamer (AuNPs-S2), the ECL of QDs nanocomposites was efficiently quenched (switch "off" state). In the presence of Hg2+ ions, formation of strong and stable T-Hg2+-T complex led to the release of the AuNPs-S2 from double-stranded DNA(dsDNA) and the recovery of the ECL signal of QDs (second signal switch "on" state). Under optimal conditions, Hg2+ can be detected in a wide linear range from 20aM to 2µM with a very low detection limit of 2aM. The proposed ECL aptasensor showed high selectivity for Hg2+ determination compared to other environmentally relevant metal ions at concentration ratio more than 1000 times. The aptasensor was used for detection Hg2+ ions from samples of tap waters, carp and saltwater fishes with satisfactory results. The aptasensor exhibited high sensitivity, wide linear response (11 orders of magnitude), excellent reproducibility and stability. The proposed aptasensor will be a promising candidate for facile and rapid determination of Hg2+in environmental and fishery samples. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/29161665/Switchable_electrochemiluminescence_aptasensor_coupled_with_resonance_energy_transfer_for_selective_attomolar_detection_of_Hg2+_via_CdTe@CdS/dendrimer_probe_and_Au_nanoparticle_quencher_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30761-3 DB - PRIME DP - Unbound Medicine ER -