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Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti.
Fish Shellfish Immunol. 2018 Feb; 73:1-10.FS

Abstract

Infectious diseases have been found to be a major cause of mortality in fish hatcheries. Probiotics have been introduced to replace antibiotics commonly used for treatment of bacterial infection in aquaculture. This study was conducted to isolate, screen, and evaluate the probiotic Bacillus spp. for potential use as a feed supplement to enhance fish growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. Bacillus aerius strain B81e was selectively isolated from the intestine of healthy catfish and chosen based on its probiotic properties both in vitro and in vivo. This bacterium produced a bacteriocin-like substance and exhibited a broad-spectrum antibacterial activity inhibiting both Gram-positive and Gram-negative bacteria especially the fish pathogens Aeromonas hydrophila and Streptococcus agalactiae. The susceptibility to all 8 antibiotics tested implies that it is unlikely to be an antibiotic-resistant bacterium. B. aerius strain B81e possessed interesting adhesion properties as shown by its high percentages of hydrophobicity, auto-aggregation, co-aggregation with fish pathogens A. hydrophila FW52 and S. agalactiae F3S and mucin binding. The strain B81e survived simulated gastrointestinal conditions, producing protease and lipase but not β-haemolysin. The study also evaluated the effects of dietary supplementation with strain B81e on growth performance, innate immunity, and the disease resistance of P. bocourti against A. hydrophila infection. Fish with a mean body weight of 69 g were fed strain B81e at 0 (control) and 107 CFU g-1 feed (test) for 60 days. Various growth and immune parameters were examined at 30 and 60 days post-feeding. Fish were challenged with A. hydrophila 60 days post-feeding and mortalities were recorded over 14 days post-infection. Results showed that the administration of strain B81e for 60 days had significant effects (p < 0.05) on weight gain, specific growth rate and feed utilization efficiency of P. bocourti. Dietary administration of strain B81e increased the serum lysozyme and bactericidal activities of P. bocourti significantly throughout the experimental period whereas the alternative complement, phagocytic and respiratory burst activities were significantly (p < 0.05) higher in the test fish compared to the control fish after 60 days of feeding. In addition, the fish fed a strain B81e supplemented diet had a significantly higher (p < 0.05) post-challenge survival rate than the control fish. The results in this study indicate that B. aerius B81e has beneficial effects on growth performance, innate immunity and disease resistance of P. bocourti. This is the first report on the probiotic roles of B. aerius in aquaculture.

Authors+Show Affiliations

Department of Microbiology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok, 10600, Thailand.Department of Science and Technology, Faculty of Arts and Science, Chaiyaphum Rajabhat University, Chaiyaphum 3600, Thailand.Department of Fisheries, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: saoton@kku.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29162546

Citation

Meidong, Ratchanu, et al. "Evaluation of Probiotic Bacillus Aerius B81e Isolated From Healthy Hybrid Catfish On Growth, Disease Resistance and Innate Immunity of Pla-mong Pangasius Bocourti." Fish & Shellfish Immunology, vol. 73, 2018, pp. 1-10.
Meidong R, Khotchanalekha K, Doolgindachbaporn S, et al. Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. Fish Shellfish Immunol. 2018;73:1-10.
Meidong, R., Khotchanalekha, K., Doolgindachbaporn, S., Nagasawa, T., Nakao, M., Sakai, K., & Tongpim, S. (2018). Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. Fish & Shellfish Immunology, 73, 1-10. https://doi.org/10.1016/j.fsi.2017.11.032
Meidong R, et al. Evaluation of Probiotic Bacillus Aerius B81e Isolated From Healthy Hybrid Catfish On Growth, Disease Resistance and Innate Immunity of Pla-mong Pangasius Bocourti. Fish Shellfish Immunol. 2018;73:1-10. PubMed PMID: 29162546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. AU - Meidong,Ratchanu, AU - Khotchanalekha,Kulwadee, AU - Doolgindachbaporn,Sompong, AU - Nagasawa,Takahiro, AU - Nakao,Miki, AU - Sakai,Kenji, AU - Tongpim,Saowanit, Y1 - 2017/11/21/ PY - 2017/08/11/received PY - 2017/11/15/revised PY - 2017/11/17/accepted PY - 2017/11/23/pubmed PY - 2018/7/26/medline PY - 2017/11/23/entrez KW - Bacillus KW - Growth KW - Immunity KW - Pangasius bocourti KW - Probiotic SP - 1 EP - 10 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 73 N2 - Infectious diseases have been found to be a major cause of mortality in fish hatcheries. Probiotics have been introduced to replace antibiotics commonly used for treatment of bacterial infection in aquaculture. This study was conducted to isolate, screen, and evaluate the probiotic Bacillus spp. for potential use as a feed supplement to enhance fish growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti. Bacillus aerius strain B81e was selectively isolated from the intestine of healthy catfish and chosen based on its probiotic properties both in vitro and in vivo. This bacterium produced a bacteriocin-like substance and exhibited a broad-spectrum antibacterial activity inhibiting both Gram-positive and Gram-negative bacteria especially the fish pathogens Aeromonas hydrophila and Streptococcus agalactiae. The susceptibility to all 8 antibiotics tested implies that it is unlikely to be an antibiotic-resistant bacterium. B. aerius strain B81e possessed interesting adhesion properties as shown by its high percentages of hydrophobicity, auto-aggregation, co-aggregation with fish pathogens A. hydrophila FW52 and S. agalactiae F3S and mucin binding. The strain B81e survived simulated gastrointestinal conditions, producing protease and lipase but not β-haemolysin. The study also evaluated the effects of dietary supplementation with strain B81e on growth performance, innate immunity, and the disease resistance of P. bocourti against A. hydrophila infection. Fish with a mean body weight of 69 g were fed strain B81e at 0 (control) and 107 CFU g-1 feed (test) for 60 days. Various growth and immune parameters were examined at 30 and 60 days post-feeding. Fish were challenged with A. hydrophila 60 days post-feeding and mortalities were recorded over 14 days post-infection. Results showed that the administration of strain B81e for 60 days had significant effects (p < 0.05) on weight gain, specific growth rate and feed utilization efficiency of P. bocourti. Dietary administration of strain B81e increased the serum lysozyme and bactericidal activities of P. bocourti significantly throughout the experimental period whereas the alternative complement, phagocytic and respiratory burst activities were significantly (p < 0.05) higher in the test fish compared to the control fish after 60 days of feeding. In addition, the fish fed a strain B81e supplemented diet had a significantly higher (p < 0.05) post-challenge survival rate than the control fish. The results in this study indicate that B. aerius B81e has beneficial effects on growth performance, innate immunity and disease resistance of P. bocourti. This is the first report on the probiotic roles of B. aerius in aquaculture. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/29162546/Evaluation_of_probiotic_Bacillus_aerius_B81e_isolated_from_healthy_hybrid_catfish_on_growth_disease_resistance_and_innate_immunity_of_Pla_mong_Pangasius_bocourti_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(17)30709-X DB - PRIME DP - Unbound Medicine ER -