Tags

Type your tag names separated by a space and hit enter

Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae.
Sci Rep. 2017 Nov 21; 7(1):15911.SR

Abstract

Aphid saliva plays important roles in aphid-host interactions, such as assisting aphid digestion, detoxification, activating or suppressing plant defenses. The grain aphid, Sitobion avenae, is one of the most devastating pests of cereals worldwide. In this study, we performed the transcriptome analysis of salivary glands of S. avenae. A total of 33,079 assembled unigenes were identified in the salivary glands of aphids. Of the all obtained unigenes, 15,833(47.86%) and 10,829(32.73%) unigenes showed high similarity to known proteins in Nr and Swiss-Prot databases respectively. 526 unigenes were predicted to encode secretory proteins, including some digestive and detoxifying enzymes and potential effectors. The RT-PCR and RT-qPCR results showed that all of the 15 most highly expressed putative secretory proteins specifically expressed in salivary glands. Interestingly, 11 of the 15 most highly expressed putative secretory proteins were still not matched to function-known proteins. We also detected the expression of 9 interested putative secretory proteins in aphid different tissues, including some digestive and detoxifying enzymes, effectors and Ca2+ binding proteins. The results showed that only glutathione-S-transferase 1 was specifically expressed in salivary glands. These findings provide a further insight into the identification of potential effectors involving in aphid-cereals interactions.

Links

PMC Free PDF

Authors+Show Affiliations

Zhang Y
State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China. Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, B-5030, Belgium.
Fan J
State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
Sun J
State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
Francis F
Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, B-5030, Belgium. Frederic.Francis@ulg.ac.be.
Chen J
State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China. jlchen@ippcaas.cn.

MeSH

AnimalsAphidsEdible GrainGene Expression ProfilingGene Expression RegulationGene OntologyMetabolic Networks and PathwaysMolecular Sequence AnnotationRNA, MessengerSalivary GlandsSequence Analysis, RNA

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29162876

Citation

Zhang, Yong, et al. "Transcriptome Analysis of the Salivary Glands of the Grain Aphid, Sitobion Avenae." Scientific Reports, vol. 7, no. 1, 2017, p. 15911.
Zhang Y, Fan J, Sun J, et al. Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae. Sci Rep. 2017;7(1):15911.
Zhang, Y., Fan, J., Sun, J., Francis, F., & Chen, J. (2017). Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae. Scientific Reports, 7(1), 15911. https://doi.org/10.1038/s41598-017-16092-z
Zhang Y, et al. Transcriptome Analysis of the Salivary Glands of the Grain Aphid, Sitobion Avenae. Sci Rep. 2017 Nov 21;7(1):15911. PubMed PMID: 29162876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae. AU - Zhang,Yong, AU - Fan,Jia, AU - Sun,Jingrui, AU - Francis,Frédéric, AU - Chen,Julian, Y1 - 2017/11/21/ PY - 2017/06/22/received PY - 2017/11/07/accepted PY - 2017/11/23/entrez PY - 2017/11/23/pubmed PY - 2019/7/10/medline SP - 15911 EP - 15911 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - Aphid saliva plays important roles in aphid-host interactions, such as assisting aphid digestion, detoxification, activating or suppressing plant defenses. The grain aphid, Sitobion avenae, is one of the most devastating pests of cereals worldwide. In this study, we performed the transcriptome analysis of salivary glands of S. avenae. A total of 33,079 assembled unigenes were identified in the salivary glands of aphids. Of the all obtained unigenes, 15,833(47.86%) and 10,829(32.73%) unigenes showed high similarity to known proteins in Nr and Swiss-Prot databases respectively. 526 unigenes were predicted to encode secretory proteins, including some digestive and detoxifying enzymes and potential effectors. The RT-PCR and RT-qPCR results showed that all of the 15 most highly expressed putative secretory proteins specifically expressed in salivary glands. Interestingly, 11 of the 15 most highly expressed putative secretory proteins were still not matched to function-known proteins. We also detected the expression of 9 interested putative secretory proteins in aphid different tissues, including some digestive and detoxifying enzymes, effectors and Ca2+ binding proteins. The results showed that only glutathione-S-transferase 1 was specifically expressed in salivary glands. These findings provide a further insight into the identification of potential effectors involving in aphid-cereals interactions. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29162876/Transcriptome_analysis_of_the_salivary_glands_of_the_grain_aphid_Sitobion_avenae_ DB - PRIME DP - Unbound Medicine ER -