Tags

Type your tag names separated by a space and hit enter

Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy.
BMC Genomics. 2017 Nov 22; 18(1):896.BG

Abstract

BACKGROUND

Parthenocarpy is an excellent agronomic trait that enables crops to set fruit in the absence of pollination and fertilization, and therefore to produce seedless fruit. Although parthenocarpy is widely recognized as a hormone-dependent process, hormone-insensitive parthenocarpy can also be observed in cucumber; however, its mechanism is poorly understood. To improve the global understanding of parthenocarpy and address the hormone-insensitive parthenocarpy shown in cucumber, we conducted a physiological and proteomic analysis of differently developed fruits.

RESULTS

Physiological analysis indicated that the natural hormone-insensitive parthenocarpy of 'EC1' has broad hormone-inhibitor resistance, and the endogenous hormones in the natural parthenocarpy (NP) fruits were stable and relatively lower than those of the non-parthenocarpic cultivar '8419 s-1.' Based on the iTRAQ technique, 683 fruit developmental proteins were identified from NP, cytokinin-induced parthenocarpic (CP), pollinated and unpollinated fruits. Gene Ontology (GO) analysis showed that proteins detected from both set and aborted fruits were involved in similar biological processes, such as cell growth, the cell cycle, cell death and communication. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that 'protein synthesis' was the major biological process that differed between fruit set and fruit abortion. Clustering analysis revealed that different protein expression patterns were involved in CP and NP fruits. Forty-one parthenocarpy-specialized DEPs (differentially expressed proteins) were screened and divided into two distinctive groups: NP-specialized proteins and CP-specialized proteins. Furthermore, qRT-PCR and western blot analysis indicated that NP-specialized proteins showed hormone- or hormone-inhibitor insensitive expression patterns in both ovaries and seedlings.

CONCLUSIONS

In this study, the global molecular regulation of fruit development in cucumber was revealed at the protein level. Physiological and proteomic comparisons indicated the presence of hormone-independent parthenocarpy and suppression of fruit abortion in cucumber. The proteomic analysis suggested that hormone-independent parthenocarpy is regulated by hormone-insensitive proteins such as the NP-specialized proteins. Moreover, the regulation of fruit abortion suppression may be closely related to protein synthesis pathways.

Authors+Show Affiliations

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. jfchen@njau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29166853

Citation

Li, Ji, et al. "Proteomic Insight Into Fruit Set of Cucumber (Cucumis Sativus L.) Suggests the Cues of Hormone-independent Parthenocarpy." BMC Genomics, vol. 18, no. 1, 2017, p. 896.
Li J, Xu J, Guo QW, et al. Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy. BMC Genomics. 2017;18(1):896.
Li, J., Xu, J., Guo, Q. W., Wu, Z., Zhang, T., Zhang, K. J., Cheng, C. Y., Zhu, P. Y., Lou, Q. F., & Chen, J. F. (2017). Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy. BMC Genomics, 18(1), 896. https://doi.org/10.1186/s12864-017-4290-5
Li J, et al. Proteomic Insight Into Fruit Set of Cucumber (Cucumis Sativus L.) Suggests the Cues of Hormone-independent Parthenocarpy. BMC Genomics. 2017 Nov 22;18(1):896. PubMed PMID: 29166853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic insight into fruit set of cucumber (Cucumis sativus L.) suggests the cues of hormone-independent parthenocarpy. AU - Li,Ji, AU - Xu,Jian, AU - Guo,Qin-Wei, AU - Wu,Zhe, AU - Zhang,Ting, AU - Zhang,Kai-Jing, AU - Cheng,Chun-Yan, AU - Zhu,Pin-Yu, AU - Lou,Qun-Feng, AU - Chen,Jin-Feng, Y1 - 2017/11/22/ PY - 2017/05/05/received PY - 2017/11/09/accepted PY - 2017/11/24/entrez PY - 2017/11/24/pubmed PY - 2018/6/22/medline KW - Cucumber (Cucumis sativus L.) KW - Hormone dependent/independent KW - Parthenocarpy KW - Proteome KW - iTRAQ SP - 896 EP - 896 JF - BMC genomics JO - BMC Genomics VL - 18 IS - 1 N2 - BACKGROUND: Parthenocarpy is an excellent agronomic trait that enables crops to set fruit in the absence of pollination and fertilization, and therefore to produce seedless fruit. Although parthenocarpy is widely recognized as a hormone-dependent process, hormone-insensitive parthenocarpy can also be observed in cucumber; however, its mechanism is poorly understood. To improve the global understanding of parthenocarpy and address the hormone-insensitive parthenocarpy shown in cucumber, we conducted a physiological and proteomic analysis of differently developed fruits. RESULTS: Physiological analysis indicated that the natural hormone-insensitive parthenocarpy of 'EC1' has broad hormone-inhibitor resistance, and the endogenous hormones in the natural parthenocarpy (NP) fruits were stable and relatively lower than those of the non-parthenocarpic cultivar '8419 s-1.' Based on the iTRAQ technique, 683 fruit developmental proteins were identified from NP, cytokinin-induced parthenocarpic (CP), pollinated and unpollinated fruits. Gene Ontology (GO) analysis showed that proteins detected from both set and aborted fruits were involved in similar biological processes, such as cell growth, the cell cycle, cell death and communication. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that 'protein synthesis' was the major biological process that differed between fruit set and fruit abortion. Clustering analysis revealed that different protein expression patterns were involved in CP and NP fruits. Forty-one parthenocarpy-specialized DEPs (differentially expressed proteins) were screened and divided into two distinctive groups: NP-specialized proteins and CP-specialized proteins. Furthermore, qRT-PCR and western blot analysis indicated that NP-specialized proteins showed hormone- or hormone-inhibitor insensitive expression patterns in both ovaries and seedlings. CONCLUSIONS: In this study, the global molecular regulation of fruit development in cucumber was revealed at the protein level. Physiological and proteomic comparisons indicated the presence of hormone-independent parthenocarpy and suppression of fruit abortion in cucumber. The proteomic analysis suggested that hormone-independent parthenocarpy is regulated by hormone-insensitive proteins such as the NP-specialized proteins. Moreover, the regulation of fruit abortion suppression may be closely related to protein synthesis pathways. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/29166853/Proteomic_insight_into_fruit_set_of_cucumber__Cucumis_sativus_L___suggests_the_cues_of_hormone_independent_parthenocarpy_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-4290-5 DB - PRIME DP - Unbound Medicine ER -