Tags

Type your tag names separated by a space and hit enter

Protective effect of ethyl acetate fraction of Drynaria quercifolia against CCl4 induced rat liver fibrosis via Nrf2/ARE and NFκB signalling pathway.
J Ethnopharmacol. 2018 Apr 24; 216:79-88.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Drynaria quercifolia rhizome is traditionally used as hepatoprotective drug especially in chronic jaundice.

AIM OF THE STUDY

The present study was undertaken to scientifically evaluate the efficacy of D. quercifolia rhizome against liver fibrosis.

MATERIALS AND METHODS

D. quercifolia rhizome crude extract (DQ) and its fractions of hexane (HDQ), ethyl acetate (EDQ), butanol (BDQ) were evaluated in vitro using primary hepatocytes and RAW 264.7 cells. In vivo anti-liver fibrotic activity of EDQ was assessed using CCl4 induced liver fibrosis in Wistar rats and serum biochemical parameters (AST, ALT, ALP, SB, cholesterol), MDA, PT, INR, GSH, SOD, CAT, liver glycogen, serum albumin levels were monitored. qRT-PCR analysis of TNF-α, COX-2, iNOS were performed. ELISA method was used to estimate TNF-α, COX-1 & 2. Histopathological studies like H & E, Masson's trichrome, immunohistochemistry staining for α-SMA, TIMP-1, Nrf2 were conducted. LC-Q-TOF-MS analysis of EDQ was conducted.

RESULTS

In vitro activity guided fractionation of D. quercifolia revealed EDQ as active fraction when compared to other extracts. EDQ treatment significantly inhibited the expression of α-SMA, TIMP-1, COX-2, TNF-α, iNOS and increased the levels of Nrf2 in rat liver fibrosis. LC-Q-TOF-MS analysis of EDQ confirmed the presence of naringin and naringenin.

CONCLUSION

The anti-liver fibrotic activity of EDQ is via inhibition of NFκB signalling pathway, antioxidant response through Nrf2 activation and further inhibition of HSC activation.

Authors+Show Affiliations

Ethnomedicine and Ethnopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerela, India. Electronic address: anuviolet@gmail.com.Ethnomedicine and Ethnopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerela, India.Ethnomedicine and Ethnopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerela, India.Ethnomedicine and Ethnopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerela, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29174446

Citation

Anuja, G I., et al. "Protective Effect of Ethyl Acetate Fraction of Drynaria Quercifolia Against CCl4 Induced Rat Liver Fibrosis Via Nrf2/ARE and NFκB Signalling Pathway." Journal of Ethnopharmacology, vol. 216, 2018, pp. 79-88.
Anuja GI, Shine VJ, Latha PG, et al. Protective effect of ethyl acetate fraction of Drynaria quercifolia against CCl4 induced rat liver fibrosis via Nrf2/ARE and NFκB signalling pathway. J Ethnopharmacol. 2018;216:79-88.
Anuja, G. I., Shine, V. J., Latha, P. G., & Suja, S. R. (2018). Protective effect of ethyl acetate fraction of Drynaria quercifolia against CCl4 induced rat liver fibrosis via Nrf2/ARE and NFκB signalling pathway. Journal of Ethnopharmacology, 216, 79-88. https://doi.org/10.1016/j.jep.2017.11.015
Anuja GI, et al. Protective Effect of Ethyl Acetate Fraction of Drynaria Quercifolia Against CCl4 Induced Rat Liver Fibrosis Via Nrf2/ARE and NFκB Signalling Pathway. J Ethnopharmacol. 2018 Apr 24;216:79-88. PubMed PMID: 29174446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of ethyl acetate fraction of Drynaria quercifolia against CCl4 induced rat liver fibrosis via Nrf2/ARE and NFκB signalling pathway. AU - Anuja,G I, AU - Shine,V J, AU - Latha,P G, AU - Suja,S R, Y1 - 2017/11/23/ PY - 2017/07/08/received PY - 2017/10/30/revised PY - 2017/11/11/accepted PY - 2017/11/28/pubmed PY - 2018/9/5/medline PY - 2017/11/28/entrez KW - Anti-inflammatory KW - Liver fibrosis KW - Nuclear factor erythroid 2-related factor 2 (Nrf2) KW - Tissue inhibitor of metalloproteinase-1 (TIMP-1) KW - Tumour necrosis factor-α (TNF-α) KW - α-Smooth muscle actin (α-SMA) SP - 79 EP - 88 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 216 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Drynaria quercifolia rhizome is traditionally used as hepatoprotective drug especially in chronic jaundice. AIM OF THE STUDY: The present study was undertaken to scientifically evaluate the efficacy of D. quercifolia rhizome against liver fibrosis. MATERIALS AND METHODS: D. quercifolia rhizome crude extract (DQ) and its fractions of hexane (HDQ), ethyl acetate (EDQ), butanol (BDQ) were evaluated in vitro using primary hepatocytes and RAW 264.7 cells. In vivo anti-liver fibrotic activity of EDQ was assessed using CCl4 induced liver fibrosis in Wistar rats and serum biochemical parameters (AST, ALT, ALP, SB, cholesterol), MDA, PT, INR, GSH, SOD, CAT, liver glycogen, serum albumin levels were monitored. qRT-PCR analysis of TNF-α, COX-2, iNOS were performed. ELISA method was used to estimate TNF-α, COX-1 & 2. Histopathological studies like H & E, Masson's trichrome, immunohistochemistry staining for α-SMA, TIMP-1, Nrf2 were conducted. LC-Q-TOF-MS analysis of EDQ was conducted. RESULTS: In vitro activity guided fractionation of D. quercifolia revealed EDQ as active fraction when compared to other extracts. EDQ treatment significantly inhibited the expression of α-SMA, TIMP-1, COX-2, TNF-α, iNOS and increased the levels of Nrf2 in rat liver fibrosis. LC-Q-TOF-MS analysis of EDQ confirmed the presence of naringin and naringenin. CONCLUSION: The anti-liver fibrotic activity of EDQ is via inhibition of NFκB signalling pathway, antioxidant response through Nrf2 activation and further inhibition of HSC activation. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/29174446/Protective_effect_of_ethyl_acetate_fraction_of_Drynaria_quercifolia_against_CCl4_induced_rat_liver_fibrosis_via_Nrf2/ARE_and_NFκB_signalling_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(17)32525-4 DB - PRIME DP - Unbound Medicine ER -