Tags

Type your tag names separated by a space and hit enter

Rapid and simple procedure for the determination of cathinones, amphetamine-like stimulants and other new psychoactive substances in blood and urine by GC-MS.
J Pharm Biomed Anal. 2018 Feb 05; 149:494-501.JP

Abstract

In the last few years an increasing number of new psychoactive substances (NPS), with different chemical structures (of which 37% are stimulants), have been released into the illicit drug market. Their detection and identification in biological samples is hence of great concern. The aim of this work was to develop a high-throughput and rapid method for the determination of different classes of stimulants (amphetamine-type stimulants, cathinones, phenethylamines and ketamine analogues) from blood and urine samples using GC-MS. The proposed method allows the almost simultaneous derivatization and extraction of analytes from biological samples in a very short time, by using hexyl chloroformate as derivatization agent. The extraction of analytes was performed by Dispersive Liquid Liquid Microextraction (DLLME), a very rapid, cheap and efficient extraction technique that employs microliter amounts of organic solvents. The chromatographic method allowed for the separation of 26 stimulants including positional isomers (3-MMC and 4-MMC). The method was validated on urine and blood samples with the ability to detect and quantify all analytes with satisfactory limits of detection (LODs) ranging between 1 and 10ng/mL, limits of quantification (LOQs) between 2 and 50ng/mL, selectivity and linearity (5-1000ng/mL). The method was then applied to real samples from forensic cases, demonstrating its suitability for the screening of a wide number of stimulants in biological specimens.

Authors+Show Affiliations

Department of Biology, University of Malta, Msida, Malta.Institute of Public Health, Section of Legal Medicine, Università Cattolica del Sacro Cuore, L.go F. Vito, 1, 00168 Rome, Italy.Department of Biology, University of Malta, Msida, Malta.Institute of Public Health, Section of Legal Medicine, Università Cattolica del Sacro Cuore, L.go F. Vito, 1, 00168 Rome, Italy. Electronic address: sabina.stranorossi@unicatt.it.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

29175556

Citation

Mercieca, Gilbert, et al. "Rapid and Simple Procedure for the Determination of Cathinones, Amphetamine-like Stimulants and Other New Psychoactive Substances in Blood and Urine By GC-MS." Journal of Pharmaceutical and Biomedical Analysis, vol. 149, 2018, pp. 494-501.
Mercieca G, Odoardi S, Cassar M, et al. Rapid and simple procedure for the determination of cathinones, amphetamine-like stimulants and other new psychoactive substances in blood and urine by GC-MS. J Pharm Biomed Anal. 2018;149:494-501.
Mercieca, G., Odoardi, S., Cassar, M., & Strano Rossi, S. (2018). Rapid and simple procedure for the determination of cathinones, amphetamine-like stimulants and other new psychoactive substances in blood and urine by GC-MS. Journal of Pharmaceutical and Biomedical Analysis, 149, 494-501. https://doi.org/10.1016/j.jpba.2017.11.024
Mercieca G, et al. Rapid and Simple Procedure for the Determination of Cathinones, Amphetamine-like Stimulants and Other New Psychoactive Substances in Blood and Urine By GC-MS. J Pharm Biomed Anal. 2018 Feb 5;149:494-501. PubMed PMID: 29175556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and simple procedure for the determination of cathinones, amphetamine-like stimulants and other new psychoactive substances in blood and urine by GC-MS. AU - Mercieca,Gilbert, AU - Odoardi,Sara, AU - Cassar,Marisa, AU - Strano Rossi,Sabina, Y1 - 2017/11/06/ PY - 2017/09/18/received PY - 2017/11/03/revised PY - 2017/11/05/accepted PY - 2017/11/28/pubmed PY - 2018/8/14/medline PY - 2017/11/28/entrez KW - Biological samples KW - Clinical toxicology KW - Dispersive Liquid Liquid Microextraction KW - Forensic toxicology KW - Gas chromatography-mass spectrometry KW - New psychoactive substances SP - 494 EP - 501 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 149 N2 - In the last few years an increasing number of new psychoactive substances (NPS), with different chemical structures (of which 37% are stimulants), have been released into the illicit drug market. Their detection and identification in biological samples is hence of great concern. The aim of this work was to develop a high-throughput and rapid method for the determination of different classes of stimulants (amphetamine-type stimulants, cathinones, phenethylamines and ketamine analogues) from blood and urine samples using GC-MS. The proposed method allows the almost simultaneous derivatization and extraction of analytes from biological samples in a very short time, by using hexyl chloroformate as derivatization agent. The extraction of analytes was performed by Dispersive Liquid Liquid Microextraction (DLLME), a very rapid, cheap and efficient extraction technique that employs microliter amounts of organic solvents. The chromatographic method allowed for the separation of 26 stimulants including positional isomers (3-MMC and 4-MMC). The method was validated on urine and blood samples with the ability to detect and quantify all analytes with satisfactory limits of detection (LODs) ranging between 1 and 10ng/mL, limits of quantification (LOQs) between 2 and 50ng/mL, selectivity and linearity (5-1000ng/mL). The method was then applied to real samples from forensic cases, demonstrating its suitability for the screening of a wide number of stimulants in biological specimens. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/29175556/Rapid_and_simple_procedure_for_the_determination_of_cathinones_amphetamine_like_stimulants_and_other_new_psychoactive_substances_in_blood_and_urine_by_GC_MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(17)32364-6 DB - PRIME DP - Unbound Medicine ER -