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An effective method for preparation of high-purity pelargonidin-3-O-glucoside from strawberry and its protective effect on cellular oxidative stress.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 01; 1072:211-220.JC

Abstract

Accumulating evidence indicates that consumption of berries may exert beneficial effects against oxidative stress mediated diseases. Pelargonidin-3-O-glucoside (Pg3G), a bioactive ingredient in strawberry, has been reported to possess a potent antioxidant capacity. This study was therefore designed to develop an effective method to prepare pure Pg3G from strawberry and investigate its protective effect against H2O2-induced oxidative stress. According to our results, Pg3G occupied 85.55% of total anthocyanin content in strawberry. 240mg of Pg3G with the purity of 97.26% was finally isolated from 320g of strawberry lyophilized powder (SLP) by combination of AB-8 macroporous resin and high-speed counter-current chromatography (HSCCC) technologies. Further study unveiled that Pg3G significantly inhibited H2O2-induced ROS generation, GSH depletion and mitochondrial dysfunction, thereby ameliorating H2O2-induced oxidative stress. Overall, this study suggests that pelargonidin-3-O-glucoside can be used as a natural bioactive agent to prevent cellular oxidative stress.

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Authors+Show Affiliations

Xu Y
Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
Hu D
Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
Li Y
Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
Sun C
Laboratory of Fruit Quality Biology, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, China.
Chen W
Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: zjuchenwei@zju.edu.cn.

MeSH

AnthocyaninsAntioxidantsCell SurvivalCountercurrent DistributionFragariaGlutathioneHep G2 CellsHumansOxidative Stress

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29179062

Citation

Xu, Yang, et al. "An Effective Method for Preparation of High-purity pelargonidin-3-O-glucoside From Strawberry and Its Protective Effect On Cellular Oxidative Stress." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1072, 2018, pp. 211-220.
Xu Y, Hu D, Li Y, et al. An effective method for preparation of high-purity pelargonidin-3-O-glucoside from strawberry and its protective effect on cellular oxidative stress. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1072:211-220.
Xu, Y., Hu, D., Li, Y., Sun, C., & Chen, W. (2018). An effective method for preparation of high-purity pelargonidin-3-O-glucoside from strawberry and its protective effect on cellular oxidative stress. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1072, 211-220. https://doi.org/10.1016/j.jchromb.2017.11.025
Xu Y, et al. An Effective Method for Preparation of High-purity pelargonidin-3-O-glucoside From Strawberry and Its Protective Effect On Cellular Oxidative Stress. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 1;1072:211-220. PubMed PMID: 29179062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An effective method for preparation of high-purity pelargonidin-3-O-glucoside from strawberry and its protective effect on cellular oxidative stress. AU - Xu,Yang, AU - Hu,Dongwen, AU - Li,Yuting, AU - Sun,Chongde, AU - Chen,Wei, Y1 - 2017/11/21/ PY - 2017/07/16/received PY - 2017/11/13/revised PY - 2017/11/18/accepted PY - 2017/11/28/pubmed PY - 2018/2/22/medline PY - 2017/11/28/entrez KW - GSH KW - High-speed counter-current chromatography KW - Oxidative stress KW - Pelargonidin-3-O-glucoside KW - Strawberry SP - 211 EP - 220 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1072 N2 - Accumulating evidence indicates that consumption of berries may exert beneficial effects against oxidative stress mediated diseases. Pelargonidin-3-O-glucoside (Pg3G), a bioactive ingredient in strawberry, has been reported to possess a potent antioxidant capacity. This study was therefore designed to develop an effective method to prepare pure Pg3G from strawberry and investigate its protective effect against H2O2-induced oxidative stress. According to our results, Pg3G occupied 85.55% of total anthocyanin content in strawberry. 240mg of Pg3G with the purity of 97.26% was finally isolated from 320g of strawberry lyophilized powder (SLP) by combination of AB-8 macroporous resin and high-speed counter-current chromatography (HSCCC) technologies. Further study unveiled that Pg3G significantly inhibited H2O2-induced ROS generation, GSH depletion and mitochondrial dysfunction, thereby ameliorating H2O2-induced oxidative stress. Overall, this study suggests that pelargonidin-3-O-glucoside can be used as a natural bioactive agent to prevent cellular oxidative stress. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/29179062/An_effective_method_for_preparation_of_high_purity_pelargonidin_3_O_glucoside_from_strawberry_and_its_protective_effect_on_cellular_oxidative_stress_ DB - PRIME DP - Unbound Medicine ER -