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Examination of biochemical and biological activities of Bothrops jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) snake venom after up to 54 years of storage.
Toxicon. 2018 Jan; 141:34-42.T

Abstract

The number of snakes donated to the Brazilian Instituto Butantan has been decreasing in the past 10 years. This circumstance motivated us to compare the properties of five venom pools of Bothrops jararaca snake stored for up to 54 years. Results showed differences among venom pools regarding enzymatic and other biological activities, such as caseinolytic, phospholipase A2, hemorrhagic and coagulant activities, as well as antigenicity. Protein content, reverse-phase chromatographic profile, and immunorecognition by commercial Bothrops antivenom were comparable for all venom pools, although lethality of the most recent preparations was higher. Since the lowest functional activities did not always correspond to older venoms, differences among venom pools used for antivenom production during the period 1963-2008 may correlate with the different proportions of venoms from different localities used in their generation, rather than to long-term storage. We conclude that B. jararaca venoms properly stored for long periods of time retain their structural and pharmacological activities, thus representing useful materials for scientific research and antivenom production.

Authors+Show Affiliations

Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil. Electronic address: dmikih@gmail.com.Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil. Electronic address: karen.zani@butantan.gov.br.Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil. Electronic address: carolineserino@hotmail.com.Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil. Electronic address: kathleen.grego@butantan.gov.br.Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil. Electronic address: savio.santanna@butantan.gov.br.Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil. Electronic address: wilbaal@gmail.com.Laboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil. Electronic address: anita.azevedo@butantan.gov.br.Departamento de Bioquímica, Universidade Federal de São Paulo, Rua 3 de Maio 100, 04044-020, São Paulo, Brazil. Electronic address: torquato.bioq@epm.br.Departamento de Bioquímica, Universidade Federal de São Paulo, Rua 3 de Maio 100, 04044-020, São Paulo, Brazil. Electronic address: tanaka.bioq@epm.br.Laboratorio de Venómica Estructural y Funcional, Consejo Superior de Investigaciones Científicas, Jaime Roig 11, 46010, Valencia, Spain. Electronic address: libia.sanz@ibv.csic.es.Laboratorio de Venómica Estructural y Funcional, Consejo Superior de Investigaciones Científicas, Jaime Roig 11, 46010, Valencia, Spain. Electronic address: jcalvete@ibv.csic.es.Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29179989

Citation

Hatakeyama, Daniela M., et al. "Examination of Biochemical and Biological Activities of Bothrops Jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) Snake Venom After Up to 54 Years of Storage." Toxicon : Official Journal of the International Society On Toxinology, vol. 141, 2018, pp. 34-42.
Hatakeyama DM, de Morais-Zani K, Serino-Silva C, et al. Examination of biochemical and biological activities of Bothrops jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) snake venom after up to 54 years of storage. Toxicon. 2018;141:34-42.
Hatakeyama, D. M., de Morais-Zani, K., Serino-Silva, C., Grego, K. F., Sant'Anna, S. S., Fernandes, W., Aniz, P. A. E. A., Torquato, R. J. S., Tanaka, A. S., Sanz, L., Calvete, J. J., & Tanaka-Azevedo, A. M. (2018). Examination of biochemical and biological activities of Bothrops jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) snake venom after up to 54 years of storage. Toxicon : Official Journal of the International Society On Toxinology, 141, 34-42. https://doi.org/10.1016/j.toxicon.2017.11.011
Hatakeyama DM, et al. Examination of Biochemical and Biological Activities of Bothrops Jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) Snake Venom After Up to 54 Years of Storage. Toxicon. 2018;141:34-42. PubMed PMID: 29179989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Examination of biochemical and biological activities of Bothrops jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) snake venom after up to 54 years of storage. AU - Hatakeyama,Daniela M, AU - de Morais-Zani,Karen, AU - Serino-Silva,Caroline, AU - Grego,Kathleen F, AU - Sant'Anna,Savio S, AU - Fernandes,Wilson, AU - Aniz,Patrícia A E A, AU - Torquato,Ricardo J S, AU - Tanaka,Aparecida S, AU - Sanz,Libia, AU - Calvete,Juan J, AU - Tanaka-Azevedo,Anita M, Y1 - 2017/11/24/ PY - 2017/09/27/received PY - 2017/11/18/revised PY - 2017/11/23/accepted PY - 2017/11/29/pubmed PY - 2019/1/10/medline PY - 2017/11/29/entrez KW - Activities of vintage venom pools KW - Bothrops jararaca snake venom KW - Long-term stored venom KW - Venom composition and stability of B. jararaca venom pools SP - 34 EP - 42 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 141 N2 - The number of snakes donated to the Brazilian Instituto Butantan has been decreasing in the past 10 years. This circumstance motivated us to compare the properties of five venom pools of Bothrops jararaca snake stored for up to 54 years. Results showed differences among venom pools regarding enzymatic and other biological activities, such as caseinolytic, phospholipase A2, hemorrhagic and coagulant activities, as well as antigenicity. Protein content, reverse-phase chromatographic profile, and immunorecognition by commercial Bothrops antivenom were comparable for all venom pools, although lethality of the most recent preparations was higher. Since the lowest functional activities did not always correspond to older venoms, differences among venom pools used for antivenom production during the period 1963-2008 may correlate with the different proportions of venoms from different localities used in their generation, rather than to long-term storage. We conclude that B. jararaca venoms properly stored for long periods of time retain their structural and pharmacological activities, thus representing useful materials for scientific research and antivenom production. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/29179989/Examination_of_biochemical_and_biological_activities_of_Bothrops_jararaca__Serpentes:_Viperidae L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(17)30357-4 DB - PRIME DP - Unbound Medicine ER -