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Effect of a fish oil diet on the composition of rat neutrophil lipids and the molecular species of choline and ethanolamine glycerophospholipids.
J Lipid Res. 1989 Jan; 30(1):77-87.JL

Abstract

When rats were fed a corn oil versus a corn oil-fish oil diet the overall phospholipid content and composition as well as the subclass distribution of the choline- and ethanolamine-containing glycerophospholipids from neutrophils were not altered. The serine-containing glycerophospholipids were characterized by high levels of stearic and oleic acids. When fish oil was added to the diet it replaced some of the arachidonate in both the inositol- and the serine-containing glycerophospholipids. In the corn oil-fed animals, 25.2 and 33.6 mole %, respectively, of the molecular species of 1,2-diacyl- and 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine contained arachidonate. The values for 1,2-diacyl and 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine were, respectively, 41 and 55.8 mole %. When half of the 5% corn oil in the diet was replaced by fish oil, there was a 53, 38, 27, and 25% reduction, respectively, in the level of arachidonate in these four lipid subclasses. The amount of 5,8,11,14,17-eicosapentaenoic acid incorporated into these four subclasses was always less than the decline in arachidonic acid. This was due, in part, to the acylation of small amounts of 22-carbon (n-3) acids into these lipids. Molecular species analysis demonstrated that 5,8,11,14,17-eicosapentaenoic acid paired with the same components at the sn-1 position, and in the same ratio, as did arachidonic acid. The amounts of 16- and 18-carbon saturated and unsaturated fatty acid at the sn-2 position were not altered by dietary change. Collectively, these findings suggest that 5,8,11,14,17-eicosapentaenoic and arachidonic acids are metabolized in a similar way by neutrophils. These studies also support the concept that neutrophils contain two metabolic pools of phospholipids. One pool is altered by dietary fat change while the pool containing 16- and 18-carbon acids is resistant to change when fish oil is included in the diet.

Authors+Show Affiliations

Department of Physiological Chemistry, College of Medicine, Ohio State University, Columbus 43210.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2918252

Citation

Careaga-Houck, M, and H Sprecher. "Effect of a Fish Oil Diet On the Composition of Rat Neutrophil Lipids and the Molecular Species of Choline and Ethanolamine Glycerophospholipids." Journal of Lipid Research, vol. 30, no. 1, 1989, pp. 77-87.
Careaga-Houck M, Sprecher H. Effect of a fish oil diet on the composition of rat neutrophil lipids and the molecular species of choline and ethanolamine glycerophospholipids. J Lipid Res. 1989;30(1):77-87.
Careaga-Houck, M., & Sprecher, H. (1989). Effect of a fish oil diet on the composition of rat neutrophil lipids and the molecular species of choline and ethanolamine glycerophospholipids. Journal of Lipid Research, 30(1), 77-87.
Careaga-Houck M, Sprecher H. Effect of a Fish Oil Diet On the Composition of Rat Neutrophil Lipids and the Molecular Species of Choline and Ethanolamine Glycerophospholipids. J Lipid Res. 1989;30(1):77-87. PubMed PMID: 2918252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of a fish oil diet on the composition of rat neutrophil lipids and the molecular species of choline and ethanolamine glycerophospholipids. AU - Careaga-Houck,M, AU - Sprecher,H, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 77 EP - 87 JF - Journal of lipid research JO - J Lipid Res VL - 30 IS - 1 N2 - When rats were fed a corn oil versus a corn oil-fish oil diet the overall phospholipid content and composition as well as the subclass distribution of the choline- and ethanolamine-containing glycerophospholipids from neutrophils were not altered. The serine-containing glycerophospholipids were characterized by high levels of stearic and oleic acids. When fish oil was added to the diet it replaced some of the arachidonate in both the inositol- and the serine-containing glycerophospholipids. In the corn oil-fed animals, 25.2 and 33.6 mole %, respectively, of the molecular species of 1,2-diacyl- and 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine contained arachidonate. The values for 1,2-diacyl and 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine were, respectively, 41 and 55.8 mole %. When half of the 5% corn oil in the diet was replaced by fish oil, there was a 53, 38, 27, and 25% reduction, respectively, in the level of arachidonate in these four lipid subclasses. The amount of 5,8,11,14,17-eicosapentaenoic acid incorporated into these four subclasses was always less than the decline in arachidonic acid. This was due, in part, to the acylation of small amounts of 22-carbon (n-3) acids into these lipids. Molecular species analysis demonstrated that 5,8,11,14,17-eicosapentaenoic acid paired with the same components at the sn-1 position, and in the same ratio, as did arachidonic acid. The amounts of 16- and 18-carbon saturated and unsaturated fatty acid at the sn-2 position were not altered by dietary change. Collectively, these findings suggest that 5,8,11,14,17-eicosapentaenoic and arachidonic acids are metabolized in a similar way by neutrophils. These studies also support the concept that neutrophils contain two metabolic pools of phospholipids. One pool is altered by dietary fat change while the pool containing 16- and 18-carbon acids is resistant to change when fish oil is included in the diet. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/2918252/Effect_of_a_fish_oil_diet_on_the_composition_of_rat_neutrophil_lipids_and_the_molecular_species_of_choline_and_ethanolamine_glycerophospholipids_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&pmid=2918252 DB - PRIME DP - Unbound Medicine ER -