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Analysis of cannabinoids in commercial hemp seed oil and decarboxylation kinetics studies of cannabidiolic acid (CBDA).
J Pharm Biomed Anal. 2018 Feb 05; 149:532-540.JP

Abstract

Hemp seed oil from Cannabis sativa L. is a very rich natural source of important nutrients, not only polyunsaturated fatty acids and proteins, but also terpenes and cannabinoids, which contribute to the overall beneficial effects of the oil. Hence, it is important to have an analytical method for the determination of these components in commercial samples. At the same time, it is also important to assess the safety of the product in terms of amount of any psychoactive cannabinoid present therein. This work presents the development and validation of a highly sensitive, selective and rapid HPLC-UV method for the qualitative and quantitative determination of the main cannabinoids, namely cannabidiolic acid (CBDA), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), tetrahydrocannabinol (THC), cannabinol (CBN), cannabigerol (CBG) and cannabidivarin (CBDV), present in 13 commercial hemp seed oils. Moreover, since decomposition of cannabinoid acids generally occurs with light, air and heat, decarboxylation studies of the most abundant acid (CBDA) were carried out in both open and closed reactor and the kinetics parameters were evaluated at different temperatures in order to evaluate the stability of hemp seed oil in different storage conditions.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Citti C
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via per Monteroni, 73100 Lecce, Italy; CNR NANOTEC, Campus Ecoteckne dell'Università del Salento, Via per Monteroni, 73100 Lecce, Italy; Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, Italy.
Pacchetti B
Linnea SA, Via Cantonale, 6595 Riazzino, Switzerland.
Vandelli MA
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, Italy.
Forni F
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, Italy.
Cannazza G
CNR NANOTEC, Campus Ecoteckne dell'Università del Salento, Via per Monteroni, 73100 Lecce, Italy; Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, Italy; FEDERCANAPA Federazione Italiana Canapa, via Genova 23°, 00184 Rome, Italy. Electronic address: giuseppe.cannazza@unimore.it.

MeSH

CannabinoidsCannabisChromatography, High Pressure LiquidDecarboxylationFood SafetyFood StoragePlant OilsPsychotropic DrugsSeedsSensitivity and SpecificitySpectrophotometry, UltravioletTandem Mass Spectrometry

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

29182999

Clinical Trial Links

Effect of a Facial Cream Containing Cannabidiol and Hemp Oil on Skin Hydration and Acne-prone Skin
Effect of an Emollient Cream Containing 0.5% Cannabidiol and 1% Hemp Oil in the Hydration and Erythema of the Skin

Citation

Citti, Cinzia, et al. "Analysis of Cannabinoids in Commercial Hemp Seed Oil and Decarboxylation Kinetics Studies of Cannabidiolic Acid (CBDA)." Journal of Pharmaceutical and Biomedical Analysis, vol. 149, 2018, pp. 532-540.
Citti C, Pacchetti B, Vandelli MA, et al. Analysis of cannabinoids in commercial hemp seed oil and decarboxylation kinetics studies of cannabidiolic acid (CBDA). J Pharm Biomed Anal. 2018;149:532-540.
Citti, C., Pacchetti, B., Vandelli, M. A., Forni, F., & Cannazza, G. (2018). Analysis of cannabinoids in commercial hemp seed oil and decarboxylation kinetics studies of cannabidiolic acid (CBDA). Journal of Pharmaceutical and Biomedical Analysis, 149, 532-540. https://doi.org/10.1016/j.jpba.2017.11.044
Citti C, et al. Analysis of Cannabinoids in Commercial Hemp Seed Oil and Decarboxylation Kinetics Studies of Cannabidiolic Acid (CBDA). J Pharm Biomed Anal. 2018 Feb 5;149:532-540. PubMed PMID: 29182999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of cannabinoids in commercial hemp seed oil and decarboxylation kinetics studies of cannabidiolic acid (CBDA). AU - Citti,Cinzia, AU - Pacchetti,Barbara, AU - Vandelli,Maria Angela, AU - Forni,Flavio, AU - Cannazza,Giuseppe, Y1 - 2017/11/20/ PY - 2017/09/04/received PY - 2017/11/14/revised PY - 2017/11/15/accepted PY - 2017/11/29/pubmed PY - 2018/8/14/medline PY - 2017/11/29/entrez KW - Cannabidiol KW - Decarboxylation kinetics KW - Hemp seed oil KW - Liquid chromatography SP - 532 EP - 540 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 149 N2 - Hemp seed oil from Cannabis sativa L. is a very rich natural source of important nutrients, not only polyunsaturated fatty acids and proteins, but also terpenes and cannabinoids, which contribute to the overall beneficial effects of the oil. Hence, it is important to have an analytical method for the determination of these components in commercial samples. At the same time, it is also important to assess the safety of the product in terms of amount of any psychoactive cannabinoid present therein. This work presents the development and validation of a highly sensitive, selective and rapid HPLC-UV method for the qualitative and quantitative determination of the main cannabinoids, namely cannabidiolic acid (CBDA), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), tetrahydrocannabinol (THC), cannabinol (CBN), cannabigerol (CBG) and cannabidivarin (CBDV), present in 13 commercial hemp seed oils. Moreover, since decomposition of cannabinoid acids generally occurs with light, air and heat, decarboxylation studies of the most abundant acid (CBDA) were carried out in both open and closed reactor and the kinetics parameters were evaluated at different temperatures in order to evaluate the stability of hemp seed oil in different storage conditions. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/29182999/Analysis_of_cannabinoids_in_commercial_hemp_seed_oil_and_decarboxylation_kinetics_studies_of_cannabidiolic_acid__CBDA__ DB - PRIME DP - Unbound Medicine ER -