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Assessment of methicillin resistant Staphylococcus Aureus detection methods: analytical comparative study.
Pan Afr Med J. 2017; 27:281.PA

Abstract

Introduction

The heterogeneous expression of methicillin resistance in Staphylococcus aureus (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA.

Methods

This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, Staphylococcus aureus strains were isolated and identified by conventional methods, and then confirmed by PCR detection of coagulase gene. PCR detection of mecA gene was used as a gold standard to assess oxacillin resistance screen agar base (ORSAB), oxacillin disc, cefoxitin disc (at different temperatures and incubation periods) and MRSA-latex agglutination test. S.aureus ATCC 25923 was used as control. Sensitivity and specificity were calculated.

Results

MRSA- latex agglutination was the most accurate test; it showed 100% of both sensitivity and specificity, followed by cefoxitin disc with sensitivity of 98.48% and specificity of 100%. However, both of oxacillin disc and oxacillin resistance screen agar base showed less accurate results, and were affected by incubation periods. Oxacillin disc after 24 h incubation both at 30°C and 35°C showed sensitivity and specificity values of 87.88% and 96.23%, respectively. However, after 48h incubation the test at 30°C showed sensitivity and specificity values of 89.39%, and 94.34%, respectively. At 35°C (48h) it showed values of 89.39%, 92.45% respectively. Specificity of ORSAB was more than oxacillin disc at 35°C after 24h incubation 98.11% and 96.23%, respectively.

Conclusion

MRSA- latex agglutination and cefoxitin disc diffusion tests are recommended for routine detection of MRSA.

Authors+Show Affiliations

Department of Microbiology, Faculty of Medicine and Health Sciences, University of El-imam El-mahdi P.O Box 209, Kosti City, Sudan.Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Khartoum University P.O. Box 11081, Khartoum, Sudan.Faculty of Sciences, Khartoum University P.O. Box 11081, Khartoum, Sudan.Department of Medical Laboratories, College of Applied Sciences, Qassim University P.O Box 1083 Burayda, Saudi Arabia.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

29187950

Citation

Ibrahim, Omer Mohammed Ali, et al. "Assessment of Methicillin Resistant Staphylococcus Aureus Detection Methods: Analytical Comparative Study." The Pan African Medical Journal, vol. 27, 2017, p. 281.
Ibrahim OMA, Bilal NE, Osman OF, et al. Assessment of methicillin resistant Staphylococcus Aureus detection methods: analytical comparative study. Pan Afr Med J. 2017;27:281.
Ibrahim, O. M. A., Bilal, N. E., Osman, O. F., & Magzoub, M. A. (2017). Assessment of methicillin resistant Staphylococcus Aureus detection methods: analytical comparative study. The Pan African Medical Journal, 27, 281. https://doi.org/10.11604/pamj.2017.27.281.9016
Ibrahim OMA, et al. Assessment of Methicillin Resistant Staphylococcus Aureus Detection Methods: Analytical Comparative Study. Pan Afr Med J. 2017;27:281. PubMed PMID: 29187950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of methicillin resistant Staphylococcus Aureus detection methods: analytical comparative study. AU - Ibrahim,Omer Mohammed Ali, AU - Bilal,Naser Eldin, AU - Osman,Omran Fadl, AU - Magzoub,Magzoub Abbas, Y1 - 2017/08/21/ PY - 2016/02/03/received PY - 2017/07/27/accepted PY - 2017/12/1/entrez PY - 2017/12/1/pubmed PY - 2017/12/9/medline KW - MRSA KW - Staphylococcus aureus KW - latex agglutination sensitivity KW - oxacillin disc test SP - 281 EP - 281 JF - The Pan African medical journal JO - Pan Afr Med J VL - 27 N2 - Introduction: The heterogeneous expression of methicillin resistance in Staphylococcus aureus (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA. Methods: This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, Staphylococcus aureus strains were isolated and identified by conventional methods, and then confirmed by PCR detection of coagulase gene. PCR detection of mecA gene was used as a gold standard to assess oxacillin resistance screen agar base (ORSAB), oxacillin disc, cefoxitin disc (at different temperatures and incubation periods) and MRSA-latex agglutination test. S.aureus ATCC 25923 was used as control. Sensitivity and specificity were calculated. Results: MRSA- latex agglutination was the most accurate test; it showed 100% of both sensitivity and specificity, followed by cefoxitin disc with sensitivity of 98.48% and specificity of 100%. However, both of oxacillin disc and oxacillin resistance screen agar base showed less accurate results, and were affected by incubation periods. Oxacillin disc after 24 h incubation both at 30°C and 35°C showed sensitivity and specificity values of 87.88% and 96.23%, respectively. However, after 48h incubation the test at 30°C showed sensitivity and specificity values of 89.39%, and 94.34%, respectively. At 35°C (48h) it showed values of 89.39%, 92.45% respectively. Specificity of ORSAB was more than oxacillin disc at 35°C after 24h incubation 98.11% and 96.23%, respectively. Conclusion: MRSA- latex agglutination and cefoxitin disc diffusion tests are recommended for routine detection of MRSA. SN - 1937-8688 UR - https://www.unboundmedicine.com/medline/citation/29187950/Assessment_of_methicillin_resistant_Staphylococcus_Aureus_detection_methods:_analytical_comparative_study_ L2 - http://www.panafrican-med-journal.com/content/article/27/281/full/ DB - PRIME DP - Unbound Medicine ER -