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Therapeutic Use of Δ9-THC and Cannabidiol: Evaluation of a New Extraction Procedure for the Preparation of Cannabis-based Olive Oil.
Curr Pharm Biotechnol. 2017; 18(10):828-833.CP

Abstract

BACKGROUND

Since 2013 Cannabis-based preparations, containing the two main cannabinoids of interest, Δ9-tetrahydrocannabinol (THC), and cannabidiol (CBD), can be used for therapeutic purposes, such as palliative care, neurodegenerative disorder treatment and other therapies. The preparations may consist of a drug partition in sachets, capsules or through the extraction in certified olive oil.

OBJECTIVE

The aims of the study were: a) to develop and validate a new liquid chromatographictandem mass spectrometric (LC-MS/MS) method for the identification and quantification of THC and CBD in olive oil; b) to evaluate the extraction efficiency and reproducibility of a new commercial extractor on the market.

METHODS

The olive oil was simply diluted three consecutive times, using organic solvents with increasing polarity index (n-hexane → isopropanol → methanol). The sample was then directly injected into LC-MS/MS system, operating in Multiple Reaction Monitoring Mode, in positive polarization. The method was then fully validated.

RESULTS

The method assessed to be linear over the range 0.1-10 ng/µL for both THC and CBD. Imprecision and accuracy were within 12.2% and 16.9% respectively; matrix effects proved to be negligible; THC concentration in oil is stable up to two months at room temperature, whenever kept in the dark. CBD provided a degradation of 30% within ten weeks. The method was then applied to olive oil after sample preparation, in order to evaluate the efficiency of extraction of a new generation instrument. Temperature of extraction is the most relevant factor to be optimized. Indeed, a difference of 2°C (from 94.5°C to 96.5°C, the highest temperature reached in the experiments) of the heating phase, increases the percentage of extraction from 54.2% to 64.0% for THC and from 58.2% to 67.0% for CBD. The amount of THC acid and CBD acid that are decarboxylated during the procedure must be check out in the future.

CONCLUSION

The developed method was simple and fast. The extraction procedure proved to be highly reproducible and applicable routinely to cannabis preparations.

Authors+Show Affiliations

Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100, Pavia, Italy.Hempcare Solutions S.R.L., via Bolama 2/a - 20126 Milano, Italy.Hempcare Solutions S.R.L., via Bolama 2/a - 20126 Milano, Italy.Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100, Pavia, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29189144

Citation

Morini, Luca, et al. "Therapeutic Use of Δ9-THC and Cannabidiol: Evaluation of a New Extraction Procedure for the Preparation of Cannabis-based Olive Oil." Current Pharmaceutical Biotechnology, vol. 18, no. 10, 2017, pp. 828-833.
Morini L, Porro G, Liso M, et al. Therapeutic Use of Δ9-THC and Cannabidiol: Evaluation of a New Extraction Procedure for the Preparation of Cannabis-based Olive Oil. Curr Pharm Biotechnol. 2017;18(10):828-833.
Morini, L., Porro, G., Liso, M., & Groppi, A. (2017). Therapeutic Use of Δ9-THC and Cannabidiol: Evaluation of a New Extraction Procedure for the Preparation of Cannabis-based Olive Oil. Current Pharmaceutical Biotechnology, 18(10), 828-833. https://doi.org/10.2174/1389201019666171129175655
Morini L, et al. Therapeutic Use of Δ9-THC and Cannabidiol: Evaluation of a New Extraction Procedure for the Preparation of Cannabis-based Olive Oil. Curr Pharm Biotechnol. 2017;18(10):828-833. PubMed PMID: 29189144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic Use of Δ9-THC and Cannabidiol: Evaluation of a New Extraction Procedure for the Preparation of Cannabis-based Olive Oil. AU - Morini,Luca, AU - Porro,Giorgio, AU - Liso,Maurizio, AU - Groppi,Angelo, PY - 2017/08/07/received PY - 2017/09/21/revised PY - 2017/11/16/accepted PY - 2017/12/1/pubmed PY - 2018/5/31/medline PY - 2017/12/1/entrez KW - LC-MS/MS KW - cannabidiol KW - cannabis preparations KW - olive oil KW - therapeutic cannabinoids KW - Δ9-tetrahydrocannabinol SP - 828 EP - 833 JF - Current pharmaceutical biotechnology JO - Curr Pharm Biotechnol VL - 18 IS - 10 N2 - BACKGROUND: Since 2013 Cannabis-based preparations, containing the two main cannabinoids of interest, Δ9-tetrahydrocannabinol (THC), and cannabidiol (CBD), can be used for therapeutic purposes, such as palliative care, neurodegenerative disorder treatment and other therapies. The preparations may consist of a drug partition in sachets, capsules or through the extraction in certified olive oil. OBJECTIVE: The aims of the study were: a) to develop and validate a new liquid chromatographictandem mass spectrometric (LC-MS/MS) method for the identification and quantification of THC and CBD in olive oil; b) to evaluate the extraction efficiency and reproducibility of a new commercial extractor on the market. METHODS: The olive oil was simply diluted three consecutive times, using organic solvents with increasing polarity index (n-hexane → isopropanol → methanol). The sample was then directly injected into LC-MS/MS system, operating in Multiple Reaction Monitoring Mode, in positive polarization. The method was then fully validated. RESULTS: The method assessed to be linear over the range 0.1-10 ng/µL for both THC and CBD. Imprecision and accuracy were within 12.2% and 16.9% respectively; matrix effects proved to be negligible; THC concentration in oil is stable up to two months at room temperature, whenever kept in the dark. CBD provided a degradation of 30% within ten weeks. The method was then applied to olive oil after sample preparation, in order to evaluate the efficiency of extraction of a new generation instrument. Temperature of extraction is the most relevant factor to be optimized. Indeed, a difference of 2°C (from 94.5°C to 96.5°C, the highest temperature reached in the experiments) of the heating phase, increases the percentage of extraction from 54.2% to 64.0% for THC and from 58.2% to 67.0% for CBD. The amount of THC acid and CBD acid that are decarboxylated during the procedure must be check out in the future. CONCLUSION: The developed method was simple and fast. The extraction procedure proved to be highly reproducible and applicable routinely to cannabis preparations. SN - 1873-4316 UR - https://www.unboundmedicine.com/medline/citation/29189144/Therapeutic_Use_of_Δ9_THC_and_Cannabidiol:_Evaluation_of_a_New_Extraction_Procedure_for_the_Preparation_of_Cannabis_based_Olive_Oil_ L2 - https://www.eurekaselect.com/157854/article DB - PRIME DP - Unbound Medicine ER -