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Amoxicillin effects on functional microbial community and spread of antibiotic resistance genes in amoxicillin manufacture wastewater treatment system.
J Environ Sci (China). 2017 Nov; 61:110-117.JE

Abstract

This study aimed to reveal how amoxicillin (AMX) affected the microbial community and the spread mechanism of antibiotic resistance genes (ARGs) in the AMX manufacture wastewater treatment system. For this purpose, a 1.47 L expanded granular sludge bed (EGSB) reactor was designed and run for 241days treating artificial AMX manufacture wastewater. 454 pyrosequencing was applied to analyze functional microorganisms in the system. The antibiotic genes OXA-1, OXA-2, OXA-10, TEM-1, CTX-M-1, class I integrons (intI1) and 16S rRNA genes were also examined in sludge samples. The results showed that the genera Ignavibacterium, Phocoenobacter, Spirochaeta, Aminobacterium and Cloacibacillus contributed to the degradation of different organic compounds (such as various sugars and amines). And the relative quantification of each β-lactam resistance gene in the study was changed with the increasing of AMX concentration. Furthermore the vertical gene transfer was the main driver for the spread of ARGs rather than horizontal transfer pathways in the system.

Authors+Show Affiliations

School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: lixiangkun@hit.edu.cn.School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.School of Municipal and Environmental Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29191308

Citation

Meng, Lingwei, et al. "Amoxicillin Effects On Functional Microbial Community and Spread of Antibiotic Resistance Genes in Amoxicillin Manufacture Wastewater Treatment System." Journal of Environmental Sciences (China), vol. 61, 2017, pp. 110-117.
Meng L, Li X, Wang X, et al. Amoxicillin effects on functional microbial community and spread of antibiotic resistance genes in amoxicillin manufacture wastewater treatment system. J Environ Sci (China). 2017;61:110-117.
Meng, L., Li, X., Wang, X., Ma, K., Liu, G., & Zhang, J. (2017). Amoxicillin effects on functional microbial community and spread of antibiotic resistance genes in amoxicillin manufacture wastewater treatment system. Journal of Environmental Sciences (China), 61, 110-117. https://doi.org/10.1016/j.jes.2017.09.020
Meng L, et al. Amoxicillin Effects On Functional Microbial Community and Spread of Antibiotic Resistance Genes in Amoxicillin Manufacture Wastewater Treatment System. J Environ Sci (China). 2017;61:110-117. PubMed PMID: 29191308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amoxicillin effects on functional microbial community and spread of antibiotic resistance genes in amoxicillin manufacture wastewater treatment system. AU - Meng,Lingwei, AU - Li,Xiangkun, AU - Wang,Xinran, AU - Ma,Kaili, AU - Liu,Gaige, AU - Zhang,Jie, Y1 - 2017/11/02/ PY - 2017/03/06/received PY - 2017/09/16/revised PY - 2017/09/25/accepted PY - 2017/12/2/entrez PY - 2017/12/2/pubmed PY - 2017/12/8/medline KW - 454 pyrosequencing KW - Amoxicillin (AMX) KW - Antibiotic resistance genes (ARGs) KW - Expanded granular sludge bed (EGSB) SP - 110 EP - 117 JF - Journal of environmental sciences (China) JO - J Environ Sci (China) VL - 61 N2 - This study aimed to reveal how amoxicillin (AMX) affected the microbial community and the spread mechanism of antibiotic resistance genes (ARGs) in the AMX manufacture wastewater treatment system. For this purpose, a 1.47 L expanded granular sludge bed (EGSB) reactor was designed and run for 241days treating artificial AMX manufacture wastewater. 454 pyrosequencing was applied to analyze functional microorganisms in the system. The antibiotic genes OXA-1, OXA-2, OXA-10, TEM-1, CTX-M-1, class I integrons (intI1) and 16S rRNA genes were also examined in sludge samples. The results showed that the genera Ignavibacterium, Phocoenobacter, Spirochaeta, Aminobacterium and Cloacibacillus contributed to the degradation of different organic compounds (such as various sugars and amines). And the relative quantification of each β-lactam resistance gene in the study was changed with the increasing of AMX concentration. Furthermore the vertical gene transfer was the main driver for the spread of ARGs rather than horizontal transfer pathways in the system. SN - 1001-0742 UR - https://www.unboundmedicine.com/medline/citation/29191308/Amoxicillin_effects_on_functional_microbial_community_and_spread_of_antibiotic_resistance_genes_in_amoxicillin_manufacture_wastewater_treatment_system L2 - https://linkinghub.elsevier.com/retrieve/pii/S1001-0742(17)32986-8 DB - PRIME DP - Unbound Medicine ER -