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A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway.
J Integr Plant Biol. 2018 Feb; 60(2):160-172.JI

Abstract

We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed splHM47). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane. OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA-seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway.

Authors+Show Affiliations

State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29193778

Citation

Zhang, Xiao-Bo, et al. "A Substitution Mutation in OsPELOTA Confers Bacterial Blight Resistance By Activating the Salicylic Acid Pathway." Journal of Integrative Plant Biology, vol. 60, no. 2, 2018, pp. 160-172.
Zhang XB, Feng BH, Wang HM, et al. A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway. J Integr Plant Biol. 2018;60(2):160-172.
Zhang, X. B., Feng, B. H., Wang, H. M., Xu, X., Shi, Y. F., He, Y., Chen, Z., Sathe, A. P., Shi, L., & Wu, J. L. (2018). A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway. Journal of Integrative Plant Biology, 60(2), 160-172. https://doi.org/10.1111/jipb.12613
Zhang XB, et al. A Substitution Mutation in OsPELOTA Confers Bacterial Blight Resistance By Activating the Salicylic Acid Pathway. J Integr Plant Biol. 2018;60(2):160-172. PubMed PMID: 29193778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway. AU - Zhang,Xiao-Bo, AU - Feng,Bao-Hua, AU - Wang,Hui-Mei, AU - Xu,Xia, AU - Shi,Yong-Feng, AU - He,Yan, AU - Chen,Zheng, AU - Sathe,Atul Prakash, AU - Shi,Lei, AU - Wu,Jian-Li, Y1 - 2018/01/17/ PY - 2017/08/13/received PY - 2017/11/24/accepted PY - 2017/12/2/pubmed PY - 2018/8/18/medline PY - 2017/12/2/entrez SP - 160 EP - 172 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 60 IS - 2 N2 - We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed splHM47). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane. OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA-seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/29193778/A_substitution_mutation_in_OsPELOTA_confers_bacterial_blight_resistance_by_activating_the_salicylic_acid_pathway_ L2 - https://doi.org/10.1111/jipb.12613 DB - PRIME DP - Unbound Medicine ER -